|Low-cost Electrochemical Microfluidic Immunoarrays for Cancer Diagnostics|
Brunah A. Otieno, Colleen E. Krause, Gregory W. Bishop, James F. Rusling
This poster describes the development and validation of a simple, low-cost, semi-automated microfluidic device for cancer biomarker protein detection to aid in cancer diagnosis and therapy monitoring.
|MsCompare: An Untargeted LC & GC/MS Metabolomics Platform for Quality Control, Precise Deconvolution and Data Analysis|
A GC/MS or LC/MS workflow for Metabolomics applications includes a number of distinct steps: experimental design, sampling, sample preparation, data acquisition, data processing, deconvolution, identification and data interpretation.
The MsXelerator software includes Quality Control procedures and Analysis of Variance (ANOVA) to properly control each step in this workflow and to obtain reliable results for both quantitation and identification.
|Design and Evaluation of High Definition Probe for HPV genotyping Microarray|
Sihn Ae Lee, Ah Reum Park, Inyoung Kim, Ji Hyung Lee, and Jongwon Kim
To improve the sensitivity and specificity of the HPV DNA Microarray, we adopted triple oligonucleotide probes for each targets and selected these probes not to have higher similarity of 75% with each others. These triple probes have shown 10 ~ 100 times higher sensitivities with comparable specificities than the conventional HPV DNA microarray of single oligonuclotide probe.
|Evidence for Integrating Point of Care Diagnostics as an Integral Part of Primary Healthcare in South African Rural Communities: A People-centred Health System-strengthening Approach|
T.P. Mashamba-Thompson, N. Moodley, M.M. Moshabela
Diagnostic systems designed in developed countries may not be immediately appropriate for use in the target developing countries. Therefore, a rigorous assessment for POC diagnostics outcomes of interest in the setting for which the test is designed should be carried out prior to adoption and scale up.
|Gut Microbial Metabolites and Hepatic Xenobiotic Metabolism: A High Throughput Screening Approach|
Glynn Martin, James Sidaway, Jonathan Swann
This poster highlights the combination of metabonomics and high throughput screening by the identification of gut microbial metabolites and a screening assay designed to determine their cytotoxicity to liver-like cell cultures.
|Digital PCR to Determine the Number of Transcripts from Single Neurons after Patch-clamp Recording|
Nóra Faragó1,2, Ágnes K. Kocsis3, Sándor Lovas3, Gábor Molnár3, Márton Rózsa3, Viktor Szemenyei3, Ágnes Zvara2, Gábor Tamás3, László G Puskás1,2
Whole-cell patch-clamp recording enables detecting electrophysiological signals from neurons, and RNA can be harvested into the patch pipette from the cells.We have optimized a dPCR protocol for determining exact transcript numbers in single neurons after patch-clamp recording by using dPCR based on high-density nanocapillary PCR.
|Low Cost EIS Methodology to Characterize Microelectrode Arrays used in Biological Applications|
C. A. Santos *, M. A. Flores*, O. Pilloni* , J. Mireles Jr**., A. Falcón ***, T. Fiordelisio ***, L. Oropeza-Ramos*
Diagnostics of electrochemical impedance in micro electrodes for biological potentials measurements is essential. A low cost EIS methodology to characterize microelectrode arrays in contact with cellular medium is presented, and results are validated by comparing them to a commercial system.
|Micro array of Ag-AgCl electrodes for cellular stimulation and sensing |
O. Pilloni*, M. A. Flores*, C. A. Santos*, A. Torres**, J. García**, H. González**, L. Oropeza-Ramos*.
Silver-Silver Chloride is investigated as a fabrication material of planar microelectrodes for sensing and stimulation of excitable cells. A theoretical design method is presented. The platform was tested for different excitable tissue confirming its feasibility.
|Human Cardiomyocytes Derived from Induced Pluripotent Stem Cells: High Throughput and High Content Assessment of Cardiac Toxicity and Drug Efficacy by Monitoring Cytosolic Free Calcium Transients|
1Kettenhofen R, 1Duenbostell A, 2Niedereichholz T, 3D’Angelo JM, 4Horai H, 5Schwengberg S, 1Bohlen H, 6Licher T"
Introduction of selected, pure human cardiomyocytes derived from induced pluripotent stem cell (hiPSCM) into a calcium transient imaging high throughput screening (HTS) assay to assess cardiotoxicity and drug efficacies.
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