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PRESEPSIN, A SOLUBLE CD14-SUBTYPE, A POSSIBLE NEW BIOMARKER INCREASES IN SEPTIC PATIENTS’ PLASMA FROM PEDIATRIC DEPARTMENT.
Hayato YAMAGUCHI1), Satoshi KIMURA1), Seiji FUKUOKA1), Emiko NAKAMA1), Hideyasu OTO2), Makoto INOUE2), Takashi SOGA2), Shigetaka KITAZAWA2), Yoh UMEDA2)

Increased plasma concentration of soluble CD14-subtype (presepsin) was observed in pediatric patients with bacteremia. Presepsin could be a possible biomarker of sepsis in pediatric patients, however, their reference interval in children could be lower than that of adults. More studies with larger number of samples are required to confirm the result.

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DETERMINATION OF HOMOCYSTEINE THIOLACTONE IN URINE BY FIELD AMPLIFIED SAMPLE INJECTION AND SWEEPING MEKC METHOD WITH UV DETECTION
Paulina Furmaniak, Pawel Kubalczyk, Kamila Borowczyk, Rafal Glowacki

* The procedure does not overload the capillary and has positive effect on the increase in peak hight,
* The method allows about 70-fold increase in method sensitivity,
* The limits of detection and quantification are 0.1 nmol and 0.09 nmol of Hcy-thiolactone in 1 mL of urine, respectively,
* Presented assay can be used for the determination of Hcy-thiolactone in biological samples.

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CAPILLARY ELECTROPHORESIS OF APIGENIN
Paulina Furmaniak, Pawel Kubalczyk, Rafal Glowacki

* Application of an appropriate mode of extraction allowed to use on-line stacking method for the determination of apigenin in plant extracts,
* The low limit of detection was 5 nmol in 1 mL of plant extract,
* Presented procedure allows approximately 20-fold increase of sensitivity,
* Elaborated assay can be useful for the determination of apigenin in biological samples.

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HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR DETERMINATION OF LIPOIC ACID IN THE FORM OF 2-S-PYRIDINIUM DERIVATIVE
Chwatko G. (1), Krawczyk M. J. (1), Iciek M. (2), Bilska-Wilkosz A. (2), Marcykiewicz B. (3), Glowacki R. (1)

* The method can be used for routine clinical monitoring of LA in human plasma and urine.
* The method was validated for urine and plasma samples.
* The imprecision, expressed as relative standard deviation value, were within range 0.8-7.7% and 0.9-6.2% for plasma and urine samples, respectively.
* The analytical recovery were from 97.3 to 117.0% for plasma and from 96.5% to 111.5% for urine samples.

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APPLICATION OF LIQUID CHROMATOGRAPHY WITH UV DETECTION FOR DETERMINATION OF PLANT GROWTH REGULATOR IN COMMON PLANT FERTILIZERS
Borowczyk K., Wyszczelska-Rokiel M., Stachniuk J., Chwatko G., Glowacki R.

1. The methodology allows facile and accurate determination of IBA in common plant fertilizer samples.
2. The analytical figures of linearity, precision and recovery shown during the method validation procedure are well within the criteria for biological sample analysis.
3. In temperature -18, 4 and 25 °C the analyte stays stable for 6 h.
4. The assay should help in control of phytohormones concentration in plants and fertilizers.

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INTERACTION BETWEEN WHEY PROTEIN NANOPARTICLES AND FATTY ACIDS
Hassan, Z.M.R*, Awad R. A.*, El-Sayed, M. M. **, Mevat I. Foda**, Otzen, D.*** and Heba H. Salama**

Nanocomplexes can be formed from WPI with good cytotoxic effect to tumer cells using cis-vaccenic and linolenic fatty acids comparable to oleic acid. It was a new interesting observation being that the nanocomplexes formed of WPI with fatty acids has a comparable cytotoxcisty to that of a-LA and ß-lg and can be used in tumor therapy.

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A Rapid and Effective Tool for Monitoring Monoclonal Antibody Production
B. Bhullar, Ph.D., Carlina Hui, B.Sc., M. Hariharan, M.Sc., J. Hewitt, B.Sc , and N. Vats, Ph.D.

Monoclonal antibodies (Mab) are used in a variety of fields from diagnostics to therapeutics. Manufacturers of in vitro diagnostics (IVD) commonly use third-party suppliers to meet their needs for specified monoclonal antibodies.

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A Novel Approach Toward Microfluidic Drug Metabolite Synthesis – Electrosynthetic Methodology Simulating Cytochrome (CYP450) Oxidation
Romain Stalder, Gregory P. Roth and Philip Podmore

A novel microfluidic technology and electrochemical synthesis method is demonstrated for the efficient generation of known drug metabolites. These metabolites are typically generated on first pass hepatic oxidation in vivo. The FLUX Module, a new microfluidic electrochemical cell manufactured by Syrris Ltd., has been employed to generate the metabolites of five commercial drugs: Tolbutamide, Chlorpromazine, Diclofenac, Primidone and Albendazole.

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MS Data Processing With Full Flexibility Of Universal Processing Software
Frans Schoutsen, Barbara van Cann, Vincent Jespers, Michal Godula

Liquid Chromatography (LC) in combination with triple quadrupole Mass Spectrometry (MS) is more and more a common analysis technique in routine laboratories. Especially when using MS/MS selected reaction monitoring (SRM) for a large number of components data handling can be a bottle neck. This poster will show MS data processing of pesticides in water samples using an already established chromatography data system which is now also capable of processing large quantities of MS data.

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Showing Results 61 - 70 of 1067
Scientific News
Brain-dwelling Worm in UK Man's Head Sequenced
Tapeworm removed from UK resident's brain reveals genetic secrets of an elusive Far East parasite.
Researchers Tease Out Glitches in Immune System's Self-Recognition
A new study revises understanding of how the process works and sheds light on autoimmune disease.
Crowdsourcing Science To Beat Psa
Scientists are hoping that crowdsourcing knowledge from the international science community will help them find the answer to a lethal kiwifruit disease.
Breakthrough in Managing Yellow Fever Disease
UC Riverside-led research could lead to antiviral therapeutics and better diagnostics to prevent spread of potentially fatal viral infection.
Themis Bioscience’s Vaccine Against Chikungunya Successful in Phase 1
Results presented at international conferences in Philadelphia and New Orleans.
Similarities and Striking Differences Found Between Mouse and Human Genome
Comparisons of both genomes’ inner workings may lead to a better use of mouse models.
New Strategy To Improve Stem Cell Transplantation
Research addresses hurdles to future transplant-based, gene-editing therapies that could help people with genetic blood diseases.
Molecular Event Mapping Opens Door to more in silico Tests
It is hoped that this new approach to mapping and predicting the impact of chemical compounds in the body could reduce the need for toxicity tests in animals.
Big Data Helps Pinpoint Possible New Stent Drug
Replacing the current drug used to coat artery-opening stents with a drug more targeted to the actual cause of stent disease could reduce blood clots and heart attacks.
Anti-Leukemia Drug May Also Work Against Ovarian Cancer
An antibody therapy already in clinical trials to treat chronic lymphocytic leukemia (CLL) may also prove effective against ovarian cancer – and likely other cancers as well.
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