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Automated, Rapid And Reliable Determination Of Dissolved Gases In Water By Static Headspace – Gas Chromatography
A. Caruso, M. Santoro, F. Bedini, P. Magni, S. Pelagatti

Purpose: Exploring the possibility to perform a quantitative determination of dissolved gases in water in a reliable and accurate way. Methods: The quantification of dissolved gases is performed by building a calibration curve spiking blank water samples with increasing quantities of saturated water followed by headspace sampling and GC/ FID analysis. Results: The results show good correlation factors and limit of quantification lower than 10ppb for the four gas analyzed.

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Coping with BIG DATA Image Formats: Integration of CBF, NeXus and HDF5, A Progress Report
Herbert J. Bernstein,[1] Jonathan M. Sloan,[2] Graeme Winter,[2] Tobias S. Richter,[2] NeXus International Advisory Committee,[3] Committee on the Maintenance of the CIF Standard[4]

The best of CBF/imgCIF (the Crystallographic Binary File, which has a complete description of the experiment), NeXus (a common data framework for neutron, X-ray and muon science, which gracefully handles large data sets) and HDF5 (Hierarchical Data Format, version 5, the high-performance data format used by NeXus) are being combined for the management of BIG DATA at synchrotrons.

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On-chip quantification of miRNA using digital droplet PCR
Q. Cai1, R.S. Wiederkehr1, B. Jones1, B. Majeed1, T. Stakenborg1, P. Fiorini1, L. Lagae1, M Tsukuda2, T. Matsuno2, I. Yamashita2

miRNAs have a great potential in diagnostics. Hence, automated profiling of miRNAs are of great interest. In-house technology show that it is possible to implement a multiplexing assay for miRNAs on a microfluidic chip using digital droplet PCR.

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New LEGENDplex™ Multi-Analyte Flow Assay Panels for Simultaneous Quantification of 13 Th Cytokines in Human and Mouse Samples
Binggang Sun, Jingjing Yao, Jiaming Hu and Shaoquan Ji

We have developed two multiplexed assay panels, human and mouse, using fluorescence–encoded beads, suitable for use on various flow cytometers. Both panels allow simultaneous quantification of 13 human or mouse cytokines, including interleukins (IL-2, 4, 5, 6, 9, 10, 13, 17A, 17F, 21, 22), IFN-? and TNF-a. These Th cytokine panels can be used for serum, plasma, cell culture supernatant and other sample types, offering useful tools for biomedical research and drug discovery.

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Multi-Color Fluorescence Microscopy Application using Novel Brilliant Violet™ and Alexa Fluor® 594 Conjugated Monoclonal Antibodies
Hong Zhang, Kelly Lundsten, Kevin Williams, Beibei Ding, Xiao Lin, Xifeng Wang, Jie Zhou, Dzung Nguyen, and John Ransom

Development of valuable new tools for the immunologist, to resolve multiple antigens simultaneously with fluorescence microscopy: Brilliant Violet 421™, Alexa Fluor® 488, Alexa Fluor® 594 and Alexa Fluor® 647 conjugated monoclonal antibodies.

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Discrimination of spectrally overlapping fluorochromes using Sony Corporation’s (SP6800) Spectral Analyzer
Divekar AA, Lee MJ, Kakuta M, Nitta N, Furuki M, Yang XF and Ransom JT

SP6800, a novel spectral analyzer from Sony Corporation, uses an innovative optics system that collects all emitted light and eliminates the need for band pass filters. Our data show that the SP6800 can perform all the analysis similar to a traditional cytometer with the added feature of separating spectrally overlapping fluorophores.

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Characterization of Antibody-Drug Conjugates by SEC with Combined Light Scattering
Chris C. Broomell

Drug Antibody Ratio (DAR) is a critical factor to the effective design of antibody-drug conjugates (ADC). This poster presents a method for DAR determination based on SEC-MALS in conjunction with UV absorption and differential refractive index detection.

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High-throughput analysis of protein formulations by DLS
Sophia Kenrick and Daniel Some

High-throughput analysis of protein formulations by Dynamic Light Scattering (DLS): thermal stability, colloidal stability and a thermal anomaly in the colloidal stability parameter D1

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GC-MS Comparison of Lavindin Grosso Oil Obtained by Steam Distillation and SFE
Claudia Worley, Lisa Zona, Susan Sonchik Marine

Oil was extracted from Lavindin Grosso using steam distillation and using supercritical fluid extraction (SFE). Multiple analyses of the same extract demonstrated repeatability of the analytical method. The variability between batches of distillate bracketed the composition obtained by SFE. The differences among the oils could be attributed more to where the lavender was grown than to the extraction method.

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Scientific News
NIH to Admit Patient Exposed to Ebola Virus for Observation
Ebola patients can be safely cared for at any hospital that follows CDC's infection control recommendations.
How to Make Stronger, “Greener” Cement
Analysis of material’s molecular structure leads to a new formula that could cut greenhouse-gas emissions.
Chemists Recruit Anthrax to Deliver Cancer Drugs
With some tinkering, a deadly protein becomes an efficient carrier for antibody drugs.
Biologists Find An Early Sign Of Cancer
Patients show boost in certain amino acids years before diagnosis of pancreatic cancer.
Researchers Develop New DNA Sequencing Method to Diagnose Tuberculosis
Teams aim to test the metagenomics technique on a wide range of samples.
UW-Madison Team Developing ‘Tissue Chip’ to Screen Neurological Toxins
A faster, more affordable way to screen for neural toxins.
A Novel Method for Portable Detection of Potent Drugs Known as ‘Bath Salts’
Researchers developed a low-cost, disposable and rapid platform for detecting bath salts.
Protein ‘Map’ Could Lead to Potent New Cancer Drugs
Findings will help scientists to design drugs that could target NMT enzyme.
Research Shows Alcohol Sensations Influenced by Genes
The research was the first to consider whether variation in the burn receptor gene might influence alcohol sensations.
Monkey Antibodies Help Fight Virus
Details of monkey antibodies against chikungunya virus could help to fight the disease in humans.
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