|Automated Tyrosine Kinase Inhibitor Cardiotoxicity Assay in Zebrafish|
Olaia Holgado, Juan Maria Virto, Isaskun Ibarbia, Patrice Dubreuil, Didier Pez, Ainhoa Letamendia, Martine Humbert, Alain Moussy, Carles Callol-Massot
The zebrafish embryos have recently gained relevance in biomedical research thanks to some of its characteristics including embryo transparency. Combined with the possibility to adapt the model with an automatic device and the reduced cost associated to each assay, the model is an ideal killer experiment in early phases of drug discovery as well as a novel method to increase the selection arguments to reduce the candidates to enter into the Drug Development process.
|ISMTR-283 Protects Human Bone Marrow Progenitors from the Lethal Effects of Ionizing Radiation|
Clarke E, Traynor-Kaplan A
Bone Marrow (BM) is extremely susceptible to radiation damage. BM from matched donors is often used to ‘rescue’ patients following medical radiation for leukemia. However, in the event of accidental/environmental radiation exposure, the ability to identify appropriate donors in a timely manner is rarely possible. Using CFC assays, we evaluated a small molecule compound (ISMTR-283) for its potential to protect the marrow progenitor cells and microenvironment from radiation damage.
|Metal Polymers, A Glue to Immobilise Proteins Onto Synthetic Surfaces|
Abernethy N, Chung E, Fontanelle BT, Gao Y, Jennins D, Koudijs MM, Lim D, Yang L, Ling T, Vukovic P, Wong A, Maeji, NJ
The main objective of this work was to develop a surface chemistry which maintains protein function and orientation per unit surface area, regardless of the surface used.
|Novel, Fully Automated Method Allows Efficient Analysis of qPCR Data for Qualitative Calling Based on Comparative Cq|
Collaborative research by Pioneer Hi-Bred (a DuPont company) and Azure PCR Limited
Assessment to ascertain if a method for analysis of qPCR data dependent on manual intervention can be replaced by automated analysis using the AzurePCRTM method.
|Volume-Related Inhibitors Standardization for Reverse Transcription Quantitative Polymerase Chain Reaction Experiments|
Pascal Pugniere, Sebastien Banzet, Thomas Chaillou, Catherine Mouret and Endre Peinnequin
This poster addresses the reliability of qPCR data and its dependence on technical variations. The proposal is that constant volume of RNA extract can improve reliability of RT-qPCR.
|Analyzing Molecular Polar Surface Descriptors to Predict Blood-Brain Barrier Permeation|
Sergey Shityakova, Winfried Neuhausa, Thomas Dandekarc and Carola Förstera
Permeation of active drugs across the vascular brain endothelium into the central nervous system (CNS) is controlled by the blood-brain barrier (BBB). Some molecular quantities like polar surface (PS) descriptors are of key interest to medicinal chemists to predict the BBB permeation fate for different drug-like chemical compounds.
|Defining off-target cleavage in a pair of Zinc Finger Nucleases|
K. Mukherjee, D. Carroll
This study looks at off-target cleavage of Zinc Finger Nucleases (ZNFs) in Drosophila in an attempt to analyze potential cleavage spots, with a view to designing more efficient ZFNs.
| Gene Expression Profiling: qPCR Toolkit for Quality Control|
Švec D., Jacobsson J., Sjöback R., Kubista M.
TATAA Biocenter explain how they developed and optimized high-throughput gene expression qPCR with ValidPrime quality control, compensating for inter-run variations.
|Processing and Managing Analytical Data to Extract and Share Knowledge – How Far We’ve Come and Why It’s So Hard?|
Michael Boruta and Sanjivanjit K. Bhal, Advanced Chemistry Development, Inc.
This paper discusses the ACD/Spectrus Processor, the new all-in-one analytical data processing and chemical characterization software.