|Early Immunogenicity Risk Mitigation|
Chloé Ackaert, Brecht Hoedemaekers, Sofie Pattijn and Philippe Stas
The ability of a drug candidate to induce an unwanted immune response can be a significant hurdle during drug development and a reason for product failures. Unwanted immunogenicity can cause loss of efficacy, altered pharmacodynamics and stability problems. .
|Mimetix® Electrospun Scaffolds for 3D Culture of Upcyte® and Primary Hepatocytes|
E. Heister, R. McKean (1) S. D. Ramachandran, B. Munst (2) B. Burkhardt, V. Haussling, A. Niissler (3)
This study investigates the Mimetix® electrospun scaffold, which consists of highly uniform electrospun PLLA microfibres supporting the three-dimensional growth of cells, as a cell-based assay system for upcyte® and primary hepatocytes.
|Novel Gpr39 Agonists: Correlation Of Binding Affinity Using Label-Free Back-Scattering Interferometry With Potency In Functional Assays|
Daniel Brown (1), Niklas Larsson (2), Ola Fjellström (3), Anders Johansson (3), Sara Lundqvist (2), Johan Brengdahl (2), and Richard J. Isaacs (1)
We describe the application of back-scattering interferometry (BSI) to the characterization of small molecule ligand binding to human GPR39 (a GPCR targeted for type-2 diabetes therapy) overexpressed in crude membrane fractions in free solution, including how BSI-derived affinity and functional assay-derived potency correlate for compounds of varying scaffolds.
|Searching for Urinary Biomarkers of Exposure to Heterocyclic Amines (HCAs)|
Rosa Busquets, Henrik L. Frandsen, Kerstin Skog
Heterocyclic amines (HCAs) are foodborne carcinogens present in cooked meat and fish, mainly. Metabolites of HCAs have been determined in urine from volunteers following a controlled meal.
|Metabolic Stability Assay Using Human Hepatocyte Co-cultures and Integrated Qualitative/Quantitative High Resolution Mass Spectrometry|
Alex Zang, Ragu Ramanathan, Cornelia Smith, Caroline Lee, Helen Shen, and Zamas Lam
Purpose: To investigate Sequential Window Acquisition of all Theoretical fragment ion spectra (SWATH™) based integrated qualitative and quantitative (qual/quant) assay for simultaneous metabolic stability and metabolite profiling assessments.
|In Vitro Species Comparison Using Long-Term Hepatocyte Co-Cultures Model and Highly Sensitive UHPLC-QTOF-MS with SWATH Analysis|
Jian Yu; Ragu Ramanathan; Cornelia Smith; Caroline Lee; Helen Shen; Zamas Lam
Purpose: To develop a reliable, quicker, and cost-saving in vitro method to accurately predict major human metabolite profile in vivo and to de-risk disproportional or unique human metabolites before a drug candidate nomination
|Metabolite Profiling Using Human Hepatocyte Co-cultures and UHPLC-QTOF-MS with Data Independent MS/MS|
Ronghua Wang, Ragu Ramanathan, Cornelia Smith, Caroline Lee, Helen Shen, and Zamas Lam
To investigate a high throughput workflow for metabolite profiling using a novel human in vitro system and advanced UHPLC-MS/MS techniques to accurately predict human metabolite profile in vivo.
|TOXICITY OF SELECTED BIOACTIVATED COMPOUNDS IN PRIMARY RAT HEPATOCYTES CULTURED IN MICROPATTERNED CO-CULTURES|
Okechukwu Ukairo, Julianne Shi, Justin Jackson, Kelly Rose, Melvin E. Andersen, and Edward L. LeCluyse
Drug Induced Liver Injury (DILI) modeled in a novel co-culture liver model. Here, we assess the bioactivation and cytotoxicity of acetaminophen (APAP) and other compounds in the 96-well rat MPCC.
|Rapid Quantification of Proteins in Complex Matrices using the DeNovix DS11 Microvolume Spectrophotometer|
Mebs A Surve & Dan Schieffer
In this poster, we will introduce the DeNovix DS-11 as the next generation in microvolume spectrophotometry.