|FDG PET/CT Utility in Gynecologic Malignancies: A comprehensive review of anatomy, pathways of metastatic spread and scan findings|
Nicholas Plaxton, MD, Aruna Polsani, MD, Raghuveer Halkar, MD, Karen Godette, MD, Barron, Bruce, MD
Our objective was to review the five major gynaecologic cancers (cervical, ovarian, uterine, vaginal, and vulvar) and demonstrate the role of FDG PET/CT in diagnosis, surveillance, FIGO staging and treatment strategy.
|FDG PET/CT Characteristics of Adrenal Benign and Malignant Lesions|
Nicholas Plaxton, MD, Raghuveer Halkar, MD, Bruce Barron, MD
We selected FDG PET/CT cases with strong key representative findings to help illustrate benign and malignant adrenal lesions. Tabular review of PET SUV values, Hounsfield units and lesion size in the different cases will be discussed.
|Hot Start dNTPs – Pushing the Limits of PCR|
Tony Le, Hidalgo Ashrafi, Sabrina Shore, Victor Timoshchuk, Natasha Paul, Richard Hogrefe, Inna Koukhareva, Alexandre Lebedev
Hot Start dNTPs are a distinct approach that employs modified nucleoside triphosphates with a thermolabile protecting group. This modification blocks low temperature primer extension and is released at higher temperatures to allow for more specific DNA polymerase incorporation.
|RNA Quality Control using the Agilent 2200 TapeStation System –Assessment of the RINe Quality Metric|
Arunkumar Padmanaban, Ruediger Salowsky, Charmian Cher
Here, we present a comparative study between the RINe quality score obtained from R6K ScreenTape and High Sensitivity R6K ScreenTape compared to the RIN quality metric obtained from the 2100 Bioanalyzer system.
|Variation in miRNA expression between TRV-infected tobacco plants is correlated with symptom severity and TRV-16K transcripts abundance|
Zhimin Yin, Krystyna Michalak, Miroslawa Chrzanowska
In the present work, expression of certain miRNAs and their targets were investigated in three tobacco plants infected with Tobacco rattle virus (TRV) isolate Deb57 collected from Northern Poland in 2008.
|Discovery of Conserved Domains for Development of Functional Markers and Identification of microRNAs from Drought Responsive Expressed Sequence Tags (ESTs) in Chickpea (Cicer arietinum L.)|
Mandape M, Saikumar K, Dudhe MY, Kumar J, Meena HP, Chauhan J, Afroz A
This stidy identified that conserved domains or gene families from the ESTs could be utilized for the identification of gene-targeted markers and for development of functional markers to accelerate marker assisted backcross breeding in chickpea.
|A Crystallographic-Based Fragment Screen Against Human BRD4 Bromodomain 1|
Hubbard P, Ritchie A, Hughes S, Lipkin M, Blackaby W & MacLeod A
Fragment-based drug discovery has now become firmly established as an important method in the lead discovery process, and is an integral component of drug discovery at BioFocus. Using a selection of compounds from our fragment collection, we have performed a crystallographic-based screen against human BRD4 bromodomain 1.
|Application of the SoftFocus® Design Strategy to Epigenetics Targets|
Sheppard D, Macritchie J, Allen V & Hughes S
Epigenetics describes the regulation of gene expression that occurs through DNA methylation and post translational modification of histones. The pharmaceutical industry has shown an explosion of interest in this field with new targets emerging.
|Fragmentor: A New Technique to Facilitate Scaffold Design for Library Generation|
Sheppard D, Macritchie J & Allen V
The requirement for novel starting points to ignite drug discovery projects is always high for emerging targets. Over the past 12 years BioFocus has built an impressive track record for the successful and original design of high quality libraries.