|Cell-Mediated Cytotoxicity Assay by High-Throughput Direct Cell Counting in Microplates using Fluorescence-Based Image Cytometry|
Leo L. Chan, Srinivas S. Somanchi, Kelsey J. Rosbach, and Dean A. Lee.
In this work, we demonstrate a novel high throughput cytotoxicity detection assay using the Celigo Imaging Cytometry method. Utilizing imaging cytometry, direct cell counting of live fluorescent target cells can be performed, which is a direct method for assessment of cytotoxicity.
|Droplet-on-Demand Platform for Biochemical Screening & Drug Discovery|
L.D. van Vliet1*, F. Gielen1, A. Sinha2, B.T. Koprowski3, J.B. Edel4, X.Niu5, A.J. deMello3, F. Hollfelder1, & J. Motschman2
To demonstrate droplet on demand applications towards study of biological entities encapsulated in nanoliter droplets. Interfacing a droplet on demand platform with microfluidic chips allows for merging and dilution of droplets. This feature is applied to encapsulate yeast cells (S. cerevisiae) and multicellular organisms (C. elegans).
|Analysis of Polymers, Additives and Contaminates in Medical Devices using Pyrolysis-GCMS|
Pyrolysis GC-MS is a standard technique used to analyze industrial polymers. This poster will show the use of multiple temperature ramps to analyze for additives and contaminants, in addition to polymer identification, in several medical devices.
|Long-term on-chip storage and release of liquid reagents for diagnostic lab-on-a-chip applications|
Daniel Czurratis*, Yvonne Beyl*, Sven Zinober*, Franz Lärmer*, Roland Zengerle**
We present a concept which enables on-chip storage of liquid reagents at room temperature and allows an easy implementation into pressure driven LoC devices.
|Investigating the Effects of Commercial Antimicrobial Agents on Human Corneal Epithelial Cell Membranes|
Ian J. Horner, Jerod J. Hurst, Nadine D. Kraut, Alyssa A. Rook, Crystal M. Collado, G Ekin-Atilla Gokcumen, and Frank V. Bright
Several commercial multi-purpose solutions (MPS) products contain polyhexamethylene biguanide (PHMB) and/or polyquaternium-1 (PQ-1) as antimicrobial agents. In this poster we report the effects of PHMB and PQ-1 on small unilamellar vesicles (SUV) that we have designed to mimic the average human corneal epithelial cell membrane.
|Direct Targets Identification of a Bioactive Compound|
Sylvain Blanc, Paul Bradley, Marie-Edith Gourdel, Michael Cholay, Gisèle Guimèse, Mike Mckenzie, George Nasi, Jean-Christophe and Barbara Ruggiero
Identifying protein partners of a small bioactive molecule is of great
interest in many aspects of life sciences and specifically in the drug
discovery and development process cycle. It is a support to (i) decipher
the mechanism of action after for example a “High Content” screening,
(ii) study “off-target” effects, (iii) adjust therapeutic indications and
clinical regimens of a drug and (iv) consider drug repositioning.
|3D-Tissue/ Whole-blood Co-culture Models Combined with Multi-Analyte Profile (MAP) Analyses for In-vivo-like Immunopharmacology|
Stein GM, Joos T, Schmolz M
Human Organotypic Test (HOT) Systems aim at in-vivo like substance characterisation of all preparations meant to act on the human immune system.
|A multiplexed amplicon sequencing technology for FFPE and circulating, cell-free DNA|
Laurie Kurihara, Catherine Couture, Julie Laliberte, Sukhinder Sandhu, Jonathan Irish, Tim Harkins and Vladimir Makarov
A novel amplicon approach allowing for hundreds of amplicons to be multiplexed in a single tube with a two workflow from sample to sequencer.
|Aptamer-Based Electrochemical Biosensing Platform for Detection of Hormonal Pollutants in Water|
Contreras Jimenez, G.; Eissa, S.; Ng, A.; Zourob, M.; Siaj, M.
A novel detection mechanism based on aptamers has been developed to detect small molecules as hormones, antibiotics, toxins, drugs, etc. For first time, an aptasensor to detect progesterone has been developed.