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Cell-Mediated Cytotoxicity Assay by High-Throughput Direct Cell Counting in Microplates using Fluorescence-Based Image Cytometry
Leo L. Chan, Srinivas S. Somanchi, Kelsey J. Rosbach, and Dean A. Lee.

In this work, we demonstrate a novel high throughput cytotoxicity detection assay using the Celigo Imaging Cytometry method. Utilizing imaging cytometry, direct cell counting of live fluorescent target cells can be performed, which is a direct method for assessment of cytotoxicity.

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Droplet-on-Demand Platform for Biochemical Screening & Drug Discovery
L.D. van Vliet1*, F. Gielen1, A. Sinha2, B.T. Koprowski3, J.B. Edel4, X.Niu5, A.J. deMello3, F. Hollfelder1, & J. Motschman2

To demonstrate droplet on demand applications towards study of biological entities encapsulated in nanoliter droplets. Interfacing a droplet on demand platform with microfluidic chips allows for merging and dilution of droplets. This feature is applied to encapsulate yeast cells (S. cerevisiae) and multicellular organisms (C. elegans).

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Analysis of Polymers, Additives and Contaminates in Medical Devices using Pyrolysis-GCMS
Gary Deger

Pyrolysis GC-MS is a standard technique used to analyze industrial polymers. This poster will show the use of multiple temperature ramps to analyze for additives and contaminants, in addition to polymer identification, in several medical devices.

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Long-term on-chip storage and release of liquid reagents for diagnostic lab-on-a-chip applications
Daniel Czurratis*, Yvonne Beyl*, Sven Zinober*, Franz Lärmer*, Roland Zengerle**

We present a concept which enables on-chip storage of liquid reagents at room temperature and allows an easy implementation into pressure driven LoC devices.

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Investigating the Effects of Commercial Antimicrobial Agents on Human Corneal Epithelial Cell Membranes
Ian J. Horner, Jerod J. Hurst, Nadine D. Kraut, Alyssa A. Rook, Crystal M. Collado, G Ekin-Atilla Gokcumen, and Frank V. Bright

Several commercial multi-purpose solutions (MPS) products contain polyhexamethylene biguanide (PHMB) and/or polyquaternium-1 (PQ-1) as antimicrobial agents. In this poster we report the effects of PHMB and PQ-1 on small unilamellar vesicles (SUV) that we have designed to mimic the average human corneal epithelial cell membrane.

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Direct Targets Identification of a Bioactive Compound
Sylvain Blanc, Paul Bradley, Marie-Edith Gourdel, Michael Cholay, Gisèle Guimèse, Mike Mckenzie, George Nasi, Jean-Christophe and Barbara Ruggiero

Identifying protein partners of a small bioactive molecule is of great
interest in many aspects of life sciences and specifically in the drug
discovery and development process cycle. It is a support to (i) decipher
the mechanism of action after for example a “High Content” screening,
(ii) study “off-target” effects, (iii) adjust therapeutic indications and
clinical regimens of a drug and (iv) consider drug repositioning.

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3D-Tissue/ Whole-blood Co-culture Models Combined with Multi-Analyte Profile (MAP) Analyses for In-vivo-like Immunopharmacology
Stein GM, Joos T, Schmolz M

Human Organotypic Test (HOT) Systems aim at in-vivo like substance characterisation of all preparations meant to act on the human immune system.

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A multiplexed amplicon sequencing technology for FFPE and circulating, cell-free DNA
Laurie Kurihara, Catherine Couture, Julie Laliberte, Sukhinder Sandhu, Jonathan Irish, Tim Harkins and Vladimir Makarov

A novel amplicon approach allowing for hundreds of amplicons to be multiplexed in a single tube with a two workflow from sample to sequencer.

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Aptamer-Based Electrochemical Biosensing Platform for Detection of Hormonal Pollutants in Water
Contreras Jimenez, G.; Eissa, S.; Ng, A.; Zourob, M.; Siaj, M.

A novel detection mechanism based on aptamers has been developed to detect small molecules as hormones, antibiotics, toxins, drugs, etc. For first time, an aptasensor to detect progesterone has been developed.

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Showing Results 71 - 80 of 1235
Scientific News
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Understand current trends and learn which application areas are expected to gain in popularity over the next few years.
‘Measuring Stick’ Standard for Gene Sequencing Now Available from NIST
New reference material, NIST RM 8398, to “map” DNA for genetic testing.
Single-cell Analysis Hits its Stride
Advances in technology and computational analysis enable scale and affordability, paving the way for translational studies.
Freshly Squeezed Vaccines
Microfluidic cell-squeezing device opens new possibilities for cell-based vaccines.
It Runs in the Family
Distantly related viruses share a common machinery for replication.
Scientists Create Mice with a Major Genetic Cause of ALS and FTD
NIH-funded study provides new platform for testing treatments for several neurodegenerative disorders.
New Target for Treating Rheumatoid Arthritis
Many affected individuals still suffer with progressive joint damage, despite treatment. This study has discovered a new treatment target which could open a door to preventing this ongoing damage.
Shining A New Light On The Immune System
Scientists at the University of St Andrews have developed a revolutionary method of identifying cells of the immune system with “molecular fingerprints” which could pave the way for the rapid detection of conditions such as leukaemia and lymphoma from a small blood sample.
Inexpensive Technique Developed to Manufacture Nanofibers
Current methods for producing nanofibers are incredibly costly and therefore limit accessibility, this new study opens the door to cheap, mass produced nanofibers.
Normal Skin Accrues High Number of Mutations Associated with Cancer
Researchers used genomic sequencing to gain insights into how somatic mutations build up in normal cells before the onset of cancer, a process that is poorly understood.
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