|ChIP-qPCR and qbasePLUS Jointly Identify a MYCN Activated miRNA Cluster in Cancer|
Barbara D’haene, Pieter Mestdagh, Daniel Muth, Frank Westerman, Frank Speleman and Jo Vandesompele
This study applies ChIP-qPCR tp assess binding of transcription factor MYCN to miRNA cluster 17-92, to positive control target MDM2, and to a negative control target region.
|Saliva’s Diagnostic Potential|
Advantages and disadvantages of using saliva as a diagnostic source of information.
|Acute Infective Endocarditis: Early Surgery versus Conventional Treatment|
Yassar Abdullah S Alamri
Infective endocarditis (IE) refers to thew inflammation of the inner layer of the heart, the endocardium, secondary to an infectious (most commonly bacterial) agent. It affects the left side of the heart more commonly than the right. IE has an estimated incidence of 1.4 to 6.2 per 10,000 persons in the developed countries, and is most commonly caused by Staphylococcus aureus, coaguase-negative staphylococcus, Viridans group streptococci and Straptococcus bovis.
|Development of an Automated Platform for HT Cloning and Expression|
Stefano Bonacci; Sara Iozzi; Scilla Buccato; Manuele Martinelli; John Telford; Domenico Maione; Roberto Petracca
Biomolecular protocols covering the whole cloning process were implemented in liquid handler robots. When compared to the manual approach, it was found that automation significantly speeds up HT cloning.
|First total synthesis of Combretastatin D-2 congeners named Isocorniculatolide A and 11-o-methylisocornicultolide|
Vijaya Kumar.Tulam, Subhash Chandra Bose. Kotte, Sanjay Kumar. Hirasker, Prathama S Mainkar, P.M. Murali and K. Mukkanti.
As a part of our ongoing research in synthesizing the Biologically important Natural Products, here we are reporting First total synthesis of newly isolated isocorniculatolide A and 11-O-methyl corniculatolide A.
|Modeling Drug Disposition of Timolol in Ocular Tissues of Rabbit following Topical Eye Drops|
S. Ray Chaudhuri, V. Lukacova, W.S. Woltosz
A serious disadvantage of ocular timolol therapy is the amount of drug getting into systemic circulation that adversely affects vital organ functions in elderly patients.
|Physiologically-based pharmacokinetic (PBPK) model for prediction of midazolam pharmacokinetics after intranasal administration in children|
Viera Lukacova,Siladitya Ray Chaudhuri, Walter S. Woltosz , Michael B. Bolger
Study predicts midazolam absorption and pharmacokinetics (PK) after intranasal (i.n.) administration in young children.
|Physiologically-based pharmacokinetic (PBPK) models for prediction of saquinavir effect on midazolam pharmacodynamics|
Viera Lukacova, Haiying Zhou, Walter S. Woltosz, Michael B. Bolger
Purpose: To predict the drug-drug interaction effect of saquinavir on midazolam pharmacodynamics (PD).
|Transporter-based In Vitro-In Vivo Extrapolation (IVIVE)|
Viera Lukacova, Michael B. Bolger, Walter S. Woltosz, Neil J. Parrott, Agnès Poirier and T. Lavè
Mechanistic analysis of in vitro measurements to obtain values of kinetic parameters for drug interactions with transporters, as well as for passive diffusion through cell membranes.