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  Posters

Automated, Rapid And Reliable Determination Of Dissolved Gases In Water By Static Headspace – Gas Chromatography
A. Caruso, M. Santoro, F. Bedini, P. Magni, S. Pelagatti

Purpose: Exploring the possibility to perform a quantitative determination of dissolved gases in water in a reliable and accurate way. Methods: The quantification of dissolved gases is performed by building a calibration curve spiking blank water samples with increasing quantities of saturated water followed by headspace sampling and GC/ FID analysis. Results: The results show good correlation factors and limit of quantification lower than 10ppb for the four gas analyzed.

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Coping with BIG DATA Image Formats: Integration of CBF, NeXus and HDF5, A Progress Report
Herbert J. Bernstein,[1] Jonathan M. Sloan,[2] Graeme Winter,[2] Tobias S. Richter,[2] NeXus International Advisory Committee,[3] Committee on the Maintenance of the CIF Standard[4]

The best of CBF/imgCIF (the Crystallographic Binary File, which has a complete description of the experiment), NeXus (a common data framework for neutron, X-ray and muon science, which gracefully handles large data sets) and HDF5 (Hierarchical Data Format, version 5, the high-performance data format used by NeXus) are being combined for the management of BIG DATA at synchrotrons.

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On-chip quantification of miRNA using digital droplet PCR
Q. Cai1, R.S. Wiederkehr1, B. Jones1, B. Majeed1, T. Stakenborg1, P. Fiorini1, L. Lagae1, M Tsukuda2, T. Matsuno2, I. Yamashita2

miRNAs have a great potential in diagnostics. Hence, automated profiling of miRNAs are of great interest. In-house technology show that it is possible to implement a multiplexing assay for miRNAs on a microfluidic chip using digital droplet PCR.

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New LEGENDplex™ Multi-Analyte Flow Assay Panels for Simultaneous Quantification of 13 Th Cytokines in Human and Mouse Samples
Binggang Sun, Jingjing Yao, Jiaming Hu and Shaoquan Ji

We have developed two multiplexed assay panels, human and mouse, using fluorescence–encoded beads, suitable for use on various flow cytometers. Both panels allow simultaneous quantification of 13 human or mouse cytokines, including interleukins (IL-2, 4, 5, 6, 9, 10, 13, 17A, 17F, 21, 22), IFN-? and TNF-a. These Th cytokine panels can be used for serum, plasma, cell culture supernatant and other sample types, offering useful tools for biomedical research and drug discovery.

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Multi-Color Fluorescence Microscopy Application using Novel Brilliant Violet™ and Alexa Fluor® 594 Conjugated Monoclonal Antibodies
Hong Zhang, Kelly Lundsten, Kevin Williams, Beibei Ding, Xiao Lin, Xifeng Wang, Jie Zhou, Dzung Nguyen, and John Ransom

Development of valuable new tools for the immunologist, to resolve multiple antigens simultaneously with fluorescence microscopy: Brilliant Violet 421™, Alexa Fluor® 488, Alexa Fluor® 594 and Alexa Fluor® 647 conjugated monoclonal antibodies.

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Discrimination of spectrally overlapping fluorochromes using Sony Corporation’s (SP6800) Spectral Analyzer
Divekar AA, Lee MJ, Kakuta M, Nitta N, Furuki M, Yang XF and Ransom JT

SP6800, a novel spectral analyzer from Sony Corporation, uses an innovative optics system that collects all emitted light and eliminates the need for band pass filters. Our data show that the SP6800 can perform all the analysis similar to a traditional cytometer with the added feature of separating spectrally overlapping fluorophores.

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Characterization of Antibody-Drug Conjugates by SEC with Combined Light Scattering
Chris C. Broomell

Drug Antibody Ratio (DAR) is a critical factor to the effective design of antibody-drug conjugates (ADC). This poster presents a method for DAR determination based on SEC-MALS in conjunction with UV absorption and differential refractive index detection.

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High-throughput analysis of protein formulations by DLS
Sophia Kenrick and Daniel Some

High-throughput analysis of protein formulations by Dynamic Light Scattering (DLS): thermal stability, colloidal stability and a thermal anomaly in the colloidal stability parameter D1

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GC-MS Comparison of Lavindin Grosso Oil Obtained by Steam Distillation and SFE
Claudia Worley, Lisa Zona, Susan Sonchik Marine

Oil was extracted from Lavindin Grosso using steam distillation and using supercritical fluid extraction (SFE). Multiple analyses of the same extract demonstrated repeatability of the analytical method. The variability between batches of distillate bracketed the composition obtained by SFE. The differences among the oils could be attributed more to where the lavender was grown than to the extraction method.

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Showing Results 81 - 90 of 1052
Scientific News
Link Between Viral Infection And Autoimmune Disease Found
Common viral infections can pave the way to autoimmune disease, Australian scientists have revealed in breakthrough research published internationally today.
Human Cancer Prognosis Is Related to Newly Identified Immune Cell
A rare population of tumor-associated “good” cells slows cancer.
Culture System Replicates Course Of Alzheimer’s Disease
Three-dimensional system should significantly reduce time and costs of drug development.
Researchers Describe New Method for Cell Line Authentication
SNP profiling, an effective method to detect misidentification, contamination, and chromosomal abnormalities in mouse cell lines.
Peptide Mimics as a Drug Target for Ebola
University of Utah researchers create a tool to develop treatments for Ebola using Protein Technologies’s peptide synthesizer.
CDC Develops a New, Faster Lab Test for Enterovirus D68
Confirmed cases will appear to rise as agency accelerates specimen testing.
Personalized Cellular Therapy Achieves Complete Remission in 90 Percent of ALL Patients Studied
University of Pennsylvania and Children's Hospital of Philadelphia studies reveal unprecedented results with investigational therapy made from patients' own immune cells.
New Lead for Potential Parkinson’s Treatment
Effects of high-risk Parkinson’s mutation are reversible.
Researchers Develop New Cells Meant to Form Blood Vessels
A technique to jump-start the body's systems for creating blood vessels for the treatment of peripheral artery disease.
New Test can Help Doctors Choose Best Treatment for Ovarian Cancer
ADNEX discriminate between benign and malignant tumours with a high level of accuracy.
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