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Monitoring Protein Synthesis in Living Cells With Fluorescent Labeled tRNA FRET Pairs


We transfect cells with tRNAs labeled as FRET donors and acceptors. A FRET signal is generated only when a donor labeled tRNA and an acceptor-labeled tRNA come in close contact (< 7 nM), as they do on the ribosome during the elongation cycle. The intensity of the FRET signal correlates with the number of ribosomes engaged in protein synthesis, providing a real-time, live-cell assay for measuring rates of protein synthesis.

Date Posted: 14/01/2013

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