ROS-Glo™ H₂O₂ Assay: A Luminescent Assay for Detection of Reactive Oxygen Species Poster

H₂O₂ is a reactive oxygen species (ROS) that is measured in cells as a marker of oxidative stress. It is also measured as a marker of enzyme activities that either consume or produce H₂O₂. It is desirable to screen chemical compounds for their capacity to alter H₂O₂ levels in cultured cells or for their effects on H₂O₂ levels in enzyme reactions. Current fluorescent assay formats are prone to false hit rates that are too high for efficient screening applications. The ROS-Glo™ luminescent H2O2 assay detects H₂O₂ directly, minimizes false hit rate and provides simple formats for cell-based and enzymatic assays.

Since various ROS are interconverted to H₂O₂ in the cell and H2O2 is the longest lived ROS, an increase in H₂O₂ can reflect a general increase in the ROS level. Our method for measuring hydrogen peroxide utilizes the H₂O₂ Substrate, which directly reacts with H₂O₂ to produce a luciferin precursor. Addition of the ROS-Glo™ Detection Solution converts the precursor to luciferin and provides luciferase and other components to produce a light signal proportional to the level of H₂O₂.