Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Products>This Product
  Products


Ligase-Free shRNA Cloning and Expression (pPS-CMV-aRFP-H1-LCS [RFP])

Product Description
Stable RNAi with shRNA lentivectors

Obtain Efficient Delivery and Permanent Knock Down
SBI’s shRNA and siRNA Lentiviral Cloning and Expression System enables you to set up stable heritable gene silencing systems. You can easily and efficietly transduce dividing and quiescent mammalian cells—including difficult-to-transfect cells such as primary, stem, neuronal, and endothelial. In addition, our RNAi vectors can be used to generate gene-specific knockdown animals, such as transgenic mice.

Analyze the Specific Effects of Target Genes
Lentiviral transduction eliminates the side effects produced by harsh, disruptive, and inefficient transfection protocols using chemically synthesized siRNA. The use of highly efficient and non-stressful lentiviral transduction greatly increases the likelihood that any observed phenotypic effects result from target-gene silencing.

Thoroughly Understand Target Gene Function
The Lentivector system provides a convenient and effective approach to create stable cell lines or transgenic animals since it efficiently integrates the siRNA expression construct of your choice into genomic DNA. Assays with cell lines or transgenic animals that permanently and heritably maintain a gene specific knock down phenotype enable you to more thoroughly analyze the specific effects resulting from silencing your target gene.

Unique, Highly Effective System
SBI’s FIV and HIV-based Cloning and Expression Lentivectors are derived from self-inactivating lentiviral vectors. This unique system offers a highly effective and very safe approach for introducing and expressing any siRNA sequence in nearly any mammalian cell system.

Single or Double Promoter Formats
The FIV and HIV-based shRNA lentivectors are both available in a single-promoter format, and they contain the lentiviral genetic elements for expression of high levels of siRNA in the target cell. In addition, we offer novel double-promoter FIV-based lentivectors which feature opposing promoters that directly generate double-stranded siRNA.

Complete Lentivector Packaging System
In order to stably transduce cells with SBI’s lentivectors, use SBI’s pPACK Lentivector Packaging System together with our 293TN cell line. The packaging system provides all the necessary coat proteins to manufacture pseudoviral particles for delivery of the siRNA expression construct into any mammalian cells or model organisms.

Product Ligase-Free shRNA Cloning and Expression (pPS-CMV-aRFP-H1-LCS [RFP])
Company System Biosciences
Price Request a quote
More Information View company product page
Catalog Number LF526A-1
Quantity 20 Reactions
Company Logo

System Biosciences
265 North Whisman Rd. Mountain View, CA 94043, USA

Tel: 1.650-968-2200
Fax: 1.650.968.2277
Email: info@systembio.com



Scientific News
Platelets are the Pathfinders for Leukocyte Extravasation During Inflammation
Findings from the study could help in the prevention and treatment of inflammatory pathologies.
ASMS 2016: Targeting Mass Spectrometry Tools for the Masses
The expanding application range of MS in life sciences, food, energy, and health sciences research was highlighted at this year's ASMS meeting in San Antonio, Texas.
Benchtop Automation Trends
Gain a better understanding of current interest in and future deployment of benchtop automated systems.
How Cancer Spreads in the Body
Cancer cells appear to depend on an unusual survival mechanism to spread around the body, according to an early study led by Queen Mary University of London.
Fix for 3-Billion-Year-Old Genetic Error
Researchers at The University of Texas at Austin have developed a fix that allows RNA to accurately proofread for the first time.
“Amazing Protein Diversity” Discovered in Maize
The genome of the corn plant – or maize, as it’s called almost everywhere except the US – “is a lot more exciting” than scientists have previously believed. So says the lead scientist in a new effort to analyze and annotate the depth of the plant’s genetic resources.
Manufactured Stem Cells to Advance Clinical Research
Clinical-grade cell line will enable development of new therapies and accelerate early-stage clinical research.
Dengue Virus Exposure May Amplify Zika Infection
Researchers at Imperial College London have found that the previous exposure to the dengue virus may increase the potency of Zika infection.
Gender Determination in Forensic Investigations
This study investigated the effectiveness of lip print analysis as a tool in gender determination.
Identifying Novel Types of Forensic Markers in Degraded DNA
Scientists have tried to verify the nucleosome protection hypothesis by discovering STRs within nucleosome core regions, using whole genome sequencing.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,600+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!