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Ligase-Free shRNA Cloning and Expression (pPS-EF1-aRFP-H1-LCS [RFP])

Product Description

Stable RNAi with shRNA lentivectors

Obtain Efficient Delivery and Permanent Knock Down
SBI’s shRNA and siRNA Lentiviral Cloning and Expression System enables you to set up stable heritable gene silencing systems. You can easily and efficietly transduce dividing and quiescent mammalian cells—including difficult-to-transfect cells such as primary, stem, neuronal, and endothelial. In addition, our RNAi vectors can be used to generate gene-specific knockdown animals, such as transgenic mice.

Analyze the Specific Effects of Target Genes
Lentiviral transduction eliminates the side effects produced by harsh, disruptive, and inefficient transfection protocols using chemically synthesized siRNA. The use of highly efficient and non-stressful lentiviral transduction greatly increases the likelihood that any observed phenotypic effects result from target-gene silencing.

Thoroughly Understand Target Gene Function
The Lentivector system provides a convenient and effective approach to create stable cell lines or transgenic animals since it efficiently integrates the siRNA expression construct of your choice into genomic DNA. Assays with cell lines or transgenic animals that permanently and heritably maintain a gene specific knock down phenotype enable you to more thoroughly analyze the specific effects resulting from silencing your target gene.

Unique, Highly Effective System
SBI’s FIV and HIV-based Cloning and Expression Lentivectors are derived from self-inactivating lentiviral vectors. This unique system offers a highly effective and very safe approach for introducing and expressing any siRNA sequence in nearly any mammalian cell system.

Single or Double Promoter Formats
The FIV and HIV-based shRNA lentivectors are both available in a single-promoter format, and they contain the lentiviral genetic elements for expression of high levels of siRNA in the target cell. In addition, we offer novel double-promoter FIV-based lentivectors which feature opposing promoters that directly generate double-stranded siRNA.

Complete Lentivector Packaging System
In order to stably transduce cells with SBI’s lentivectors, use SBI’s pPACK Lentivector Packaging System together with our 293TN cell line. The packaging system provides all the necessary coat proteins to manufacture pseudoviral particles for delivery of the siRNA expression construct into any mammalian cells or model organisms.

Product Ligase-Free shRNA Cloning and Expression (pPS-EF1-aRFP-H1-LCS [RFP])
Company System Biosciences
Price Request a quote
More Information View company product page
Catalog Number LF527A-1
Quantity 20 Reactions
Company Logo

System Biosciences
265 North Whisman Rd. Mountain View, CA 94043, USA

Tel: 1.650-968-2200
Fax: 1.650.968.2277

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