Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Products>This Product
  Products


pGreenPuro Scramble Hairpin Control - Construct (for shRNAs and miRZips)

Product Description

Stable RNAi with shRNA lentivectors

Obtain Efficient Delivery and Permanent Knock Down
SBI’s shRNA and siRNA Lentiviral Cloning and Expression System enables you to set up stable heritable gene silencing systems. You can easily and efficietly transduce dividing and quiescent mammalian cells—including difficult-to-transfect cells such as primary, stem, neuronal, and endothelial. In addition, our RNAi vectors can be used to generate gene-specific knockdown animals, such as transgenic mice.

Analyze the Specific Effects of Target Genes
Lentiviral transduction eliminates the side effects produced by harsh, disruptive, and inefficient transfection protocols using chemically synthesized siRNA. The use of highly efficient and non-stressful lentiviral transduction greatly increases the likelihood that any observed phenotypic effects result from target-gene silencing.

Thoroughly Understand Target Gene Function
The Lentivector system provides a convenient and effective approach to create stable cell lines or transgenic animals since it efficiently integrates the siRNA expression construct of your choice into genomic DNA. Assays with cell lines or transgenic animals that permanently and heritably maintain a gene specific knock down phenotype enable you to more thoroughly analyze the specific effects resulting from silencing your target gene.

Unique, Highly Effective System
SBI’s FIV and HIV-based Cloning and Expression Lentivectors are derived from self-inactivating lentiviral vectors. This unique system offers a highly effective and very safe approach for introducing and expressing any siRNA sequence in nearly any mammalian cell system.

Single or Double Promoter Formats
The FIV and HIV-based shRNA lentivectors are both available in a single-promoter format, and they contain the lentiviral genetic elements for expression of high levels of siRNA in the target cell. In addition, we offer novel double-promoter FIV-based lentivectors which feature opposing promoters that directly generate double-stranded siRNA.

Complete Lentivector Packaging System
In order to stably transduce cells with SBI’s lentivectors, use SBI’s pPACK Lentivector Packaging System together with our 293TN cell line. The packaging system provides all the necessary coat proteins to manufacture pseudoviral particles for delivery of the siRNA expression construct into any mammalian cells or model organisms.

Product pGreenPuro Scramble Hairpin Control - Construct (for shRNAs and miRZips)
Company System Biosciences
Price Request a quote
More Information View company product page
Catalog Number MZIP000-PA-1
Quantity Bacterial Streak
Company Logo

System Biosciences
265 North Whisman Rd. Mountain View, CA 94043, USA

Tel: 1.650-968-2200
Fax: 1.650.968.2277
Email: info@systembio.com



Scientific News
Mass Spec Technology Drives Innovation Across the Biopharma Workflow
With greater resolving power, analytical speed, and accuracy, new mass spectrometry technology and techniques are infiltrating the biopharmaceuticals workflow.
One Step Closer to Precision Medicine for Chronic Lung Disease Sufferers
A study led by University of North Carolina at Chapel Hill, and National Jewish Health, has provided evidence of links between SNPs and known COPD blood protein biomarkers.
Blood Pressure Drug May Boost Effectiveness of Lung Cancer Treatment
Researchers at Imperial College London have suggested that the blood pressure drug may make a type of lung cancer treatment more effective.
Gene Regulation in Brain May Explain Repetitive Behaviors in Rett Syndrome Patients
The research could be a key step in developing treatments to eliminate symptoms that drastically impair the quality of life in Rett patients.
Heart Arrhythmia Caused by Mosaic of Mutant Cells
Researchers have solved the genetic mystery of an infant suffering from heart arrhythmia.
Iron Nanoparticles Make Immune Cells Attack Cancer
Researchers accidentally discover that nanoparticles invented for anemia treatment can trigger the immune system’s ability to destroy tumor cells.
Crispr Toolbox Expanded By Protein
Researchers have shown a newly discovered CRISPR protein has two distinct RNA cutting activities.
CES Score May Predict Response to Cancer Treatment
Researchers identify new type of biomarker that helps predict prognosis and response to several types of cancer treatment.
Uncovering Cancer’s ‘Invisibility Cloak’
Researchers discover cancer cell mechanism to become invisible to the body's immune system.
Genetic Impact of Endurance Training
Research has found that endurance training changes genetic activity in thousands of genes, giving rise to large number of altered RNA variants.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!