Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Products>This Product
  Products


pGreenPuro shRNA expression lentivector (CMV)

Product Description

Stable RNAi with shRNA lentivectors

Obtain Efficient Delivery and Permanent Knock Down
SBI’s shRNA and siRNA Lentiviral Cloning and Expression System enables you to set up stable heritable gene silencing systems. You can easily and efficietly transduce dividing and quiescent mammalian cells—including difficult-to-transfect cells such as primary, stem, neuronal, and endothelial. In addition, our RNAi vectors can be used to generate gene-specific knockdown animals, such as transgenic mice.

Analyze the Specific Effects of Target Genes
Lentiviral transduction eliminates the side effects produced by harsh, disruptive, and inefficient transfection protocols using chemically synthesized siRNA. The use of highly efficient and non-stressful lentiviral transduction greatly increases the likelihood that any observed phenotypic effects result from target-gene silencing.

Thoroughly Understand Target Gene Function
The Lentivector system provides a convenient and effective approach to create stable cell lines or transgenic animals since it efficiently integrates the siRNA expression construct of your choice into genomic DNA. Assays with cell lines or transgenic animals that permanently and heritably maintain a gene specific knock down phenotype enable you to more thoroughly analyze the specific effects resulting from silencing your target gene.

Unique, Highly Effective System
SBI’s FIV and HIV-based Cloning and Expression Lentivectors are derived from self-inactivating lentiviral vectors. This unique system offers a highly effective and very safe approach for introducing and expressing any siRNA sequence in nearly any mammalian cell system.

Single or Double Promoter Formats
The FIV and HIV-based shRNA lentivectors are both available in a single-promoter format, and they contain the lentiviral genetic elements for expression of high levels of siRNA in the target cell. In addition, we offer novel double-promoter FIV-based lentivectors which feature opposing promoters that directly generate double-stranded siRNA.

Complete Lentivector Packaging System
In order to stably transduce cells with SBI’s lentivectors, use SBI’s pPACK Lentivector Packaging System together with our 293TN cell line. The packaging system provides all the necessary coat proteins to manufacture pseudoviral particles for delivery of the siRNA expression construct into any mammalian cells or model organisms.

Product pGreenPuro shRNA expression lentivector (CMV)
Company System Biosciences
Price Request a quote
More Information View company product page
Catalog Number SI505VA-1
Quantity >1 x 10^6 IFUs
Company Logo

System Biosciences
265 North Whisman Rd. Mountain View, CA 94043, USA

Tel: 1.650-968-2200
Fax: 1.650.968.2277
Email: info@systembio.com



Scientific News
RNAi Screening Trends
Understand current trends and learn which application areas are expected to gain in popularity over the next few years.
The Genetic Roots of Adolescent Scoliosis
Scientists at the RIKEN Center for Integrative Medical Sciences in collaboration with Keio University in Japan have discovered a gene that is linked to susceptibility of Scoliosis.
A Gene-Sequence Swap Using CRISPR to Cure Haemophilia
For the first time chromosomal defects responsible for hemophilia have been corrected in patient-specific iPSCs using CRISPR-Cas9 nucleases
Experimental MERS Vaccine Shows Promise in Animal Studies
A two-step regimen of experimental vaccines against Middle East respiratory syndrome (MERS) prompted immune responses in mice and rhesus macaques, report National Institutes of Health scientists who designed the vaccines.
New Tool Uses 'Drug Spillover' to Match Cancer Patients with Treatments
Researchers have developed a new tool that improves the ability to match drugs to disease: the Kinase Addiction Ranker (KAR) predicts what genetics are truly driving the cancer in any population of cells and chooses the best "kinase inhibitor" to silence these dangerous genetic causes of disease.
Understanding the Molecular Origin of Epigenetic Markers
Researchers at IRB Barcelona discover the molecular mechanism that determines how epigenetic markers influence gene expression.
HIV Susceptibility Linked to Little-Understood Immune Cell Class
High levels of diversity among immune cells called natural killer cells may strongly predispose people to infection by HIV, and may be driven by prior viral exposures, according to a new study.
Diagnostic Test Developed for Enterovirus D68
researchers at Washington University School of Medicine in St. Louis have developed a diagnostic test to quickly detect enterovirus D68 (EV-D68), a respiratory virus that caused unusually severe illness in children last year.
How a Kernel Got Naked and Corn Became King
Ten thousand years ago, a golden grain got naked, brought people together and grew to become one of the top agricultural commodities on the planet.
Sweet Revenge Against Superbugs
A special type of synthetic sugar could be the latest weapon in the fight against superbugs.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!