Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Products>This Product
  Products


GeneNet Human 50K siRNA Library in pSIF1-H1-copGFP

Product Description

Pooled lentiviral library content

    * 200,000 siRNA sequences targeting 47,400 human mRNA transcripts
    * Selectable puromycin resistance allows positive identification and selection of transduced cells
    * Pre-packaged, ready-to-use format—transduce your cells the day you receive the library
    * High-throughput identification of functional siRNAs by Affymetrix GeneChip® hybridization
    * Choose from siRNA libraries in FIV or HIV-based lentivectors
    * Also available in plasmid form, you can now produce large quantities of packaged siRNA library in your own lab

The GeneNet™ Human 50K siRNA Library contains 200,000 siRNA templates targeted to 47,400 human transcripts listed in the NCBI RefSeq database. For most of the target genes, four different siRNA sequences were designed using our proprietary algorithm. The library is available cloned into the FIV-based pSIF1-H1-copGFP shRNA Expression Vectors for fluorescence-based sorting, or in the FIV-based pSIF1-H1-Puro or HIV-based pSIH1-H1-Puro shRNA Expression Vectors, which confer resistance to the antibiotic puromycin. This single-promoter siRNA vector expresses cloned short-hairpin RNA (shRNA) from a single H1 RNA polymerase III promoter.

The library is available in both pre-packaged and plasmid form. The pre-packaged VSV-G pseudotyped viral stock can be used to transduce your cells of choice immediately upon receipt, or be stored at -70oC for several months. With the library in plasmid form, you can perform the pseudoviral packaging step in your own cell culture facility. Enough plasmid is provided to yield about 109 ifu of packaged siRNA library.

After transducing the library into your cells of interest, you select those cells that display the desired phenotype or response using whichever technique is appropriate for your system. Following selection, the lentiviral inserts—containing the siRNA templates—are easily recovered by PCR and identified using the GeneChip® Human Genome U133 Plus 2.0 Array (Cat.# 900470) manufactured by Affymetrix (Santa Clara, CA). The siRNA template sequences were designed to hybridize to oligonucleotide probes on this array to facilitate identification. Although more tedious, you can also use sequencing to identify the particular siRNA sequences in selected cells.

To confirm representation, siRNA templates for the library were packaged in viral particles, amplified, labeled, and hybridized to the Affymetrix Human Genome U133 Plus 2.0 Array. Similarly, siRNA templates were amplified from the genomic DNA extracted from target cells transduced with the library. These templates were also labeled and hybridized to the Human Genome U133 Plus 2.0 Array. Overall, in both the viral- and cell-derived samples, more than 55% of the gene-specific siRNA template species used in construction of the library were easily detectable above background. In addition, the correlation of the relative amounts of different siRNA template species between both samples was very high, indicating that there is not appreciable loss or change in representation with transduction. These results indicate that the library delivers a representative number of siRNA sequences that target the majority of genes for which the library was originally designed. Also, the high correlation between the pre- and post-transduction samples shows that the pseudotyped viral particles transduce with similar efficiency, regardless of the siRNA template they harbor.

Product GeneNet Human 50K siRNA Library in pSIF1-H1-copGFP
Company System Biosciences
Price Request a quote
More Information View company product page
Catalog Number SI205B-1
Quantity >2x 10^7 IFUs
Company Logo

System Biosciences
265 North Whisman Rd. Mountain View, CA 94043, USA

Tel: 1.650-968-2200
Fax: 1.650.968.2277
Email: info@systembio.com



Scientific News
Liquid Biopsies: Miracle Diagnostic or Next New Fad?
Thanks to the development of highly specific gene-amplification and sequencing technologies liquid biopsies access more biomarkers relevant to more cancers than ever before.
Core-Shell Columns in HPLC: Food Analysis Applications
Explore the most recent applications of core-shell columns in food analysis.
Review of the Analysis of Haemoglobin A1c for Diabetes Diagnostics
This paper aims to clarify methods, units, quality requirements, reference and cutoff limits for hemoglobin A1c (HbA1c) and ratio of blood glucose/HbA1c on the basis of the results from Finnish quality control surveys by comparing them to the literature.
Colon Cancer Blocked in Mice
Case Western Reserve University Researchers block common type of colon cancer tumour in mice, laying groundwork for human clinical trial.
New Centre Offers Ultra-Speed Protein Analysis
UW-Madison researchers to establish development centre for next-gen protein measurement technologies.
Disrupting Tumour-Promotion in Humans
Researchers have modified an existing protein to represses a specific cancer-promoting ‘message’ within cells.
Protein Nanocages Could Improve Drug Design and Delivery
HHMI scientists have designed and built 10 large protein icosahedra that are similar to viral capsids that carry viral DNA.
Vaccine Strategy Targets Multiple Influenza Viruses
Scientists have identified vaccine-induced antibodies that can neutralize strains of influenza virus that infect humans.
Connectome Map More Than Doubles Human Cortex’s Known Regions
Researchers at NIH have developed software that automatically detects the “fingerprint” of each of these areas in an individual’s brain scans.
Discovered Through ‘Big Data’ Analysis
Researchers at the SBP have identified over 100 new genetic regions that affect the immune response to cancer.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,300+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,800+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!