Corporate Banner
Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>Products>This Product

GeneNet Human 50K Plasmid siRNA Library in pSIF1-H1-copGFP

Product Description

Pooled lentiviral library content

    * 200,000 siRNA sequences targeting 47,400 human mRNA transcripts
    * Selectable puromycin resistance allows positive identification and selection of transduced cells
    * Pre-packaged, ready-to-use format—transduce your cells the day you receive the library
    * High-throughput identification of functional siRNAs by Affymetrix GeneChip® hybridization
    * Choose from siRNA libraries in FIV or HIV-based lentivectors
    * Also available in plasmid form, you can now produce large quantities of packaged siRNA library in your own lab

The GeneNet™ Human 50K siRNA Library contains 200,000 siRNA templates targeted to 47,400 human transcripts listed in the NCBI RefSeq database. For most of the target genes, four different siRNA sequences were designed using our proprietary algorithm. The library is available cloned into the FIV-based pSIF1-H1-copGFP shRNA Expression Vectors for fluorescence-based sorting, or in the FIV-based pSIF1-H1-Puro or HIV-based pSIH1-H1-Puro shRNA Expression Vectors, which confer resistance to the antibiotic puromycin. This single-promoter siRNA vector expresses cloned short-hairpin RNA (shRNA) from a single H1 RNA polymerase III promoter.

The library is available in both pre-packaged and plasmid form. The pre-packaged VSV-G pseudotyped viral stock can be used to transduce your cells of choice immediately upon receipt, or be stored at -70oC for several months. With the library in plasmid form, you can perform the pseudoviral packaging step in your own cell culture facility. Enough plasmid is provided to yield about 109 ifu of packaged siRNA library.

After transducing the library into your cells of interest, you select those cells that display the desired phenotype or response using whichever technique is appropriate for your system. Following selection, the lentiviral inserts—containing the siRNA templates—are easily recovered by PCR and identified using the GeneChip® Human Genome U133 Plus 2.0 Array (Cat.# 900470) manufactured by Affymetrix (Santa Clara, CA). The siRNA template sequences were designed to hybridize to oligonucleotide probes on this array to facilitate identification. Although more tedious, you can also use sequencing to identify the particular siRNA sequences in selected cells.

To confirm representation, siRNA templates for the library were packaged in viral particles, amplified, labeled, and hybridized to the Affymetrix Human Genome U133 Plus 2.0 Array. Similarly, siRNA templates were amplified from the genomic DNA extracted from target cells transduced with the library. These templates were also labeled and hybridized to the Human Genome U133 Plus 2.0 Array. Overall, in both the viral- and cell-derived samples, more than 55% of the gene-specific siRNA template species used in construction of the library were easily detectable above background. In addition, the correlation of the relative amounts of different siRNA template species between both samples was very high, indicating that there is not appreciable loss or change in representation with transduction. These results indicate that the library delivers a representative number of siRNA sequences that target the majority of genes for which the library was originally designed. Also, the high correlation between the pre- and post-transduction samples shows that the pseudotyped viral particles transduce with similar efficiency, regardless of the siRNA template they harbor.

Product GeneNet Human 50K Plasmid siRNA Library in pSIF1-H1-copGFP
Company System Biosciences
Price Request a quote
More Information View company product page
Catalog Number SI205PB-1
Quantity 200ug
Company Logo

System Biosciences
265 North Whisman Rd. Mountain View, CA 94043, USA

Tel: 1.650-968-2200
Fax: 1.650.968.2277

Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
How a Genetic Locus Protects Adult Blood-Forming Stem Cells
Mammalian imprinted Gtl2 protects adult hematopoietic stem cells by restricting metabolic activity in the cells' mitochondria.
Genetic Basis of Fatal Flu Side Effect Discovered
A group of people with fatal H1N1 flu died after their viral infections triggered a deadly hyperinflammatory disorder in susceptible individuals with gene mutations linked to the overactive immune response, according to a recent study.
New Tech Vastly Improves CRISPR/Cas9 Accuracy
A new CRISPR/Cas9 technology developed by scientists at UMass Medical School is precise enough to surgically edit DNA at nearly any genomic location, while avoiding potentially harmful off-target changes typically seen in standard CRISPR gene editing techniques.
The MaxSignal Colistin ELISA Test Kit from Bioo Scientific
Kit can help prevent the antibiotic apocalypse by keeping last resort drugs out of the food supply.
"Good" Mozzie Virus Might Hold Key to Fighting Human Disease
Australian scientists have discovered a new virus carried by one of the country’s most common pest mosquitoes.
Non-Disease Proteins Kill Brain Cells
Scientists at the forefront of cutting-edge research into neurodegenerative diseases such as Alzheimer’s and Parkinson’s have shown that the mere presence of protein aggregates may be as important as their form and identity in inducing cell death in brain tissue.
Closing the Loop on an HIV Escape Mechanism
Research team finds that protein motions regulate virus infectivity.
New Class of RNA Tumor Suppressors Identified
Two short, “housekeeping” RNA molecules block cancer growth by binding to an important cancer-associated protein called KRAS. More than a quarter of all human cancers are missing these RNAs.
Potential Treatment for Life-Threatening Viral Infections Revealed
The findings point to new therapies for Dengue, West Nile and Ebola.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos