Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Products>This Product
  Products


GeneNet Mouse 40K siRNA Library in pSIF1-H1-Puro

Product Description

Pooled lentiviral library content

    * 150,000 siRNA sequences targeting 39,000 mouse mRNA transcripts
    * Selectable puromycin resistance allows positive selection of transduced cells
    * Pre-packaged, ready-to-use format—transduce your cells the day you receive the library
    * High-throughput identification of functional siRNAs by Affymetrix GeneChip® hybridization
    * Choose from siRNA libraries in FIV or HIV-based lentivectors
    * Also available in plasmid form, you can now produce large quantities of packaged siRNA library in your own lab

The GeneNet™ Mouse 40K siRNA Library contains 150,000 siRNA templates targeted to 39,000 mouse transcripts listed in the NCBI RefSeq database. For most of the target genes, four different siRNA sequences were designed using our proprietary algorithm. The library is available cloned into the FIV-based pSIF1-H1-Puro or HIV-based pSIH1-H1-Puro shRNA Expression Vectors, which confer resistance to the antibiotic puromycin. This expresses cloned short-hairpin RNA (shRNA) from a single H1 RNA polymerase III promoter.

The library is available in both pre-packaged and plasmid form. The pre-packaged VSV-G pseudotyped viral stock can be used to transduce your cells of choice immediately upon receipt, or be stored at -70oC for several months. With the library in plasmid form, you can perform the pseudoviral packaging step in your own cell culture facility. Enough plasmid is provided to yield about 109 ifu of packaged siRNA library.

After transducing the library into your cells of interest, you select those cells that display the desired phenotype or response using whichever technique is appropriate for your system. Following selection, the lentiviral inserts—containing the siRNA templates—are easily recovered by PCR and identified using the GeneChip® Mouse Genome 430 2.0 Array (Cat.# 900495) manufactured by Affymetrix (Santa Clara, CA). The siRNA template sequences were designed to hybridize to oligonucleotide probes on this array to facilitate identification. Although more tedious, you can also use sequencing to identify the particular siRNA sequences in selected cells.

To confirm representation, siRNA templates for the library were packaged in viral particles, amplified, labeled, and hybridized to the Affymetrix Mouse Genome 430 2.0 Array . Similarly, siRNA templates were amplified from the genomic DNA extracted from target cells transduced with the library. These templates were also labeled and hybridized to the Mouse Genome 430 2.0 Array. Overall, in both the viral- and cell-derived samples, more than 55% of the gene-specific siRNA template species used in construction of the library were easily detectable above background. In addition, the correlation of the relative amounts of different siRNA template species between both samples was very high, indicating that there is not appreciable loss or change in representation with transduction. These results indicate that the library delivers a representative number of siRNA sequences that target the majority of genes for which the library was originally designed. Also, the high correlation between the pre- and post-transduction samples shows that the pseudotyped viral particles transduce with similar efficiency, regardless of the siRNA template they harbor.

Product GeneNet Mouse 40K siRNA Library in pSIF1-H1-Puro
Company System Biosciences
Price Request a quote
More Information View company product page
Catalog Number SI222B-1
Quantity >2x 10^7 IFUs
Company Logo

System Biosciences
265 North Whisman Rd. Mountain View, CA 94043, USA

Tel: 1.650-968-2200
Fax: 1.650.968.2277
Email: info@systembio.com



Scientific News
Shedding Light on HIV Vaccine Design
Broadly speaking - Mathematical modelling of host-pathogen coevolution sheds light on HIV vaccine design.
AACC 2016 Sees Clinical Chemistry Labs Drive Precision Medicine Offerings
Biomarker assays to enable precision medicine and risk assessment, mass spec-based tests designed for use in clinical labs large and small, and liquid biopsy technology captured the spotlight at the AACC annual meeting.
Automated Patch Clamping Trends
Learn more about current practices, preferences and metrics in ion channel drug screening using APC technology.
Lab-on-a-Stick: Miniaturised Clinical Testing For Fast Detection Of Antibiotic Resistance
A portable power-free test for the rapid detection of bacterial resistance to antibiotics has been developed by academics at Loughborough University and the University of Reading.
Genetic Ancestry of Cultivated Strawberry Unravelled
UNH scientists constructed a linkage map of the seven chromosomes of the diploid Fragaria iinumae, which allows them to fill in a piece of the genetic puzzle about the eight sets of chromosomes of the cultivated strawberry.
Progress In Vaccination Against Vespid Venom
Researchers at the Helmholtz Zentrum München and the Technical University Munich have presented a method which facilitates a personalised procedure for wasp allergy sufferers.
New Drug Target for Inflammatory Disorders
Penn study finds enigmatic molecules maintain equilibrium between fighting infection and inflammatory havoc.
Breast Cancer Cells Found To Switch Molecular Characteristics
Spontaneous interconversion between HER2-positive and HER2-negative states could contribute to progression, treatment resistance in breast cancer.
Mechanisms of Calcium Blockers
Researchers describe how the fundamental mode of action of two distinct chemical classes of calcium channel blockers differs.
Some Breast Cancer Patients With Low Genetic Risk Could Skip Chemotherapy
Genetic test can help predict survival and guide treatment options.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,300+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,900+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!