Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>Products>This Product

Human Oct4 Differentiation Reporter (pRedZeo, pre-packaged)

Product Description
Accurately monitor the pluripotent state Mammalian development requires the specification of over 200 unique cell types from a single totipotent cell. Embryonic stem (ES) cells are derived from the inner cell mass of the developing blastocyst and can be propagated in culture in an undifferentiated state while maintaining the capacity to generate any cell type in the body. The recent derivation of human ES cells provides a unique opportunity to study early development and an understanding of the transcriptional regulatory circuitry that is responsible for pluripotency and self-renewal in human ES cells is fundamental to understanding human development and realizing the therapeutic potential of these cells. Three transcription factors are known to be critical in the maintenance of ESC pluripotency: Oct4, Nanog, and Sox2. Oct4 (Pou5f1) has a highly conserved role in maintaining pluripotent cell populations (Nichols et al., 1998; Morrison and Brickman, 2006), and its expression level dictates ESC fate (Niwa et al., 2000). SOX2 forms a complex with OCT4 and is necessary to cooperatively activate target genes in ESCs (Yuan et al., 1995; Ambrosetti et al., 1997). These factors comprise one essential circuit regulating ESC pluripotency in which OCT4 regulates Sox2, and additionally, the OCT4-SOX2 complex activates Oct4 expression (Okumura-Nakanishi et al., 2005). The transcription factors OCT4, SOX2, and NANOG have essential roles in early development and are required for the propagation of undifferentiated embryonic stem (ES) cells in culture. SBI has built lentivector-based transciptional Response Reporters that are responsive to Oct4 or Sox2 activity as response elements or Promoter Reporters for either Human or Mouse Oct4 and Nanog into the pGreenZeo, pRedZeo and pRedTK systems. Choice of fluorescent screening marker The lenti-reporter systems employ either a Green Fluorescent Protein (copGFP) or a Red Fluorescent Protein (RFP). Depending upon your screen, either Green or Red markers may be suitable and compatible with other fluorescent protein markers in your experimental scheme. The Response Reporters feature the dual reporting system with both GFP and Luciferase. Choice of antibiotic selection marker The Response Reporters have an optional constitutive EF1-Puro or -Neo selection cassette for establishing cells lines. The Promoter Reporters feature Zeomycin (Zeo) resistance marker confers resistance to the antibiotic Zeocin™. Zeocin™ causes cell death by intercalating into DNA and causes DNA strand breakage. This antibiotic is effective on most aerobic cells and is therefore useful for selection in bacteria, eukaryotic microorganisms, plant and animal cells. Thymidine kinase (TK) is a Herpes simplex virus-derived enzyme that acts on the guanosine nucleotide analog ganciclovir. TK phosphorylates ganciclovir, and TK+ cells produce highly-toxic triphosphates that lead to cell death (negative selection). All Stem Cell Pluipotency Monitors are available as lentivector plasmids or as pre-packaged lentiviral preparations (using the HIV lentiviral backbone and pseudotyped with VSV-G protein)—ready for transduction of target cells. The standard product size is 10 ug of plasmid or 1 x 10^6 IFUs per packaged lentiviral reporter. Positive and negative transduction control plasmids and pre-packaged viral particles are also available.
Product Human Oct4 Differentiation Reporter (pRedZeo, pre-packaged)
Company System Biosciences
Price Request a quote
More Information View company product page
Catalog Number SR10043VA-1
Quantity >2 x 10^6 IFUs
Company Logo

System Biosciences
265 North Whisman Rd. Mountain View, CA 94043, USA

Tel: 1.650-968-2200
Fax: 1.650.968.2277

Scientific News
Integrated Omics Analysis
Studying multi-omics promises to give a more holistic picture of the organism and its place in its ecosystem, however despite the complexities involved those within the field are optimistic.
Unravelling the Role of Key Genes and DNA Methylation in Blood Cell Malignancies
Researchers from the University of Nebraska Medical Center have demonstrated the role of Dnmt3a in safeguarding normal haematopoiesis.
Salford Lung Study - The First Real World Clinical Trial
In this podcast, we learn about the Salford Lung Study and its potential to revolutionize the way we assess new drugs and treatments around the world.
Point of Care Diagnostics - A Cautious Revolution
Advances in molecular biology, coupled with the miniaturization and improved sensitivity of assays and devices in general, have enabled a new wave of point-of-care (POC) or “bedside” diagnostics.
Mass Spec Technology Drives Innovation Across the Biopharma Workflow
With greater resolving power, analytical speed, and accuracy, new mass spectrometry technology and techniques are infiltrating the biopharmaceuticals workflow.
Structure of Primary Cannabinoid Receptor is Revealed
The findings provide key insights into how natural and synthetic cannabinoids including tetrahydrocannabinol —a primary chemical in marijuana—bind at the CB1 receptor to produce their effects.
Overlooked Molecules Could Revolutionise our Understanding of the Immune System
Researchers have discovered that around one third of all the epitopes displayed for scanning by the immune system are a type known as ‘spliced’ epitopes.
Illumina Contributes to ClinVar Database
The contribution includes variants of all classifications, from pathogenic to benign, identified during interpretation of whole genome sequences generated in the CLIA-certified, CAP-accredited Illumina Clinical Services Laboratory.
Agilent Presents Early Career Professor Award to Dr. Roeland Verhaak
JAX professor recognized for the development and implementation of workflows for the analysis of big-data from transcriptomics to next generation sequencing approaches.
NIH Study Determines Key Differences between Allergic and Non-Allergic Dust Mite Proteins
Researchers at NIH have uncovered factors that lead to the development of dust mite allergy and assist in the design of better allergy therapies.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,100+ scientific videos