Corporate Banner
Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>Products>This Product

Mouse Oct4 Differentiation Reporter (pRedZeo, pre-packaged)

Product Description
Accurately monitor the pluripotent state Mammalian development requires the specification of over 200 unique cell types from a single totipotent cell. Embryonic stem (ES) cells are derived from the inner cell mass of the developing blastocyst and can be propagated in culture in an undifferentiated state while maintaining the capacity to generate any cell type in the body. The recent derivation of human ES cells provides a unique opportunity to study early development and an understanding of the transcriptional regulatory circuitry that is responsible for pluripotency and self-renewal in human ES cells is fundamental to understanding human development and realizing the therapeutic potential of these cells. Three transcription factors are known to be critical in the maintenance of ESC pluripotency: Oct4, Nanog, and Sox2. Oct4 (Pou5f1) has a highly conserved role in maintaining pluripotent cell populations (Nichols et al., 1998; Morrison and Brickman, 2006), and its expression level dictates ESC fate (Niwa et al., 2000). SOX2 forms a complex with OCT4 and is necessary to cooperatively activate target genes in ESCs (Yuan et al., 1995; Ambrosetti et al., 1997). These factors comprise one essential circuit regulating ESC pluripotency in which OCT4 regulates Sox2, and additionally, the OCT4-SOX2 complex activates Oct4 expression (Okumura-Nakanishi et al., 2005). The transcription factors OCT4, SOX2, and NANOG have essential roles in early development and are required for the propagation of undifferentiated embryonic stem (ES) cells in culture. SBI has built lentivector-based transciptional Response Reporters that are responsive to Oct4 or Sox2 activity as response elements or Promoter Reporters for either Human or Mouse Oct4 and Nanog into the pGreenZeo, pRedZeo and pRedTK systems. Choice of fluorescent screening marker The lenti-reporter systems employ either a Green Fluorescent Protein (copGFP) or a Red Fluorescent Protein (RFP). Depending upon your screen, either Green or Red markers may be suitable and compatible with other fluorescent protein markers in your experimental scheme. The Response Reporters feature the dual reporting system with both GFP and Luciferase. Choice of antibiotic selection marker The Response Reporters have an optional constitutive EF1-Puro or -Neo selection cassette for establishing cells lines. The Promoter Reporters feature Zeomycin (Zeo) resistance marker confers resistance to the antibiotic Zeocin™. Zeocin™ causes cell death by intercalating into DNA and causes DNA strand breakage. This antibiotic is effective on most aerobic cells and is therefore useful for selection in bacteria, eukaryotic microorganisms, plant and animal cells. Thymidine kinase (TK) is a Herpes simplex virus-derived enzyme that acts on the guanosine nucleotide analog ganciclovir. TK phosphorylates ganciclovir, and TK+ cells produce highly-toxic triphosphates that lead to cell death (negative selection). All Stem Cell Pluipotency Monitors are available as lentivector plasmids or as pre-packaged lentiviral preparations (using the HIV lentiviral backbone and pseudotyped with VSV-G protein)—ready for transduction of target cells. The standard product size is 10 ug of plasmid or 1 x 10^6 IFUs per packaged lentiviral reporter. Positive and negative transduction control plasmids and pre-packaged viral particles are also available.
Product Mouse Oct4 Differentiation Reporter (pRedZeo, pre-packaged)
Company System Biosciences
Price Request a quote
More Information View company product page
Catalog Number SR10045VA-1
Quantity >2 x 10^6 IFUs
Company Logo

System Biosciences
265 North Whisman Rd. Mountain View, CA 94043, USA

Tel: 1.650-968-2200
Fax: 1.650.968.2277

Scientific News
Breaking Through the Barriers to Lab Innovation
Here we examine the drivers behind the move for greater innovation, the challenges and current trends in laboratory informatics, and the tools that can be used to break these barriers.
Education and Expense: The Barriers to Mass Spectrometry in Clinical Laboratories?
Here we examine the perceived barriers to mass spec in clinical laboratories and explore the possible drivers behind the recent shift in uptake of the technology in clinical settings.
Fruit Fly Pheromone Flags Great Real Estate for Starting a Family
Finding could aid efforts to control mosquito-borne diseases like malaria by manipulating odorants
Gene Editing Could Enable Pig-To-Human Organ Transplant
The largest number of simultaneous gene edits ever accomplished in the genome could help bridge the gap between organ transplant scarcity and the countless patients who need them.
Antioxidants Cause Malignant Melanoma to Metastasize Faster
Fresh research at Sahlgrenska Academy has found that antioxidants can double the rate of melanoma metastasis in mice.
New Therapy Reduces Symptoms of Inherited Enzyme Deficiency
A phase three clinical trial of a new enzyme replacement medication, sebelipase alfa, showed a reduction in multiple disease-related symptoms in children and adults with lysosomal acid lipase deficiency, an inherited enzyme deficiency that can result in scarring of the liver and high cholesterol.
Adult High Blood Pressure Risk Identifiable in Childhood
Groups of people at risk of having high blood pressure and other related health issues by age 38 can be identified in childhood, new University of Otago research suggests.
Analyzing Protein Structures in Their Native Environment
Enhanced-sensitivity NMR could reveal new clues to how proteins fold.
Supercoiled DNA is Far More Dynamic Than the “Watson-Crick” Double Helix
Researchers have imaged in unprecedented detail the three-dimensional structure of supercoiled DNA, revealing that its shape is much more dynamic than the well-known double helix.
Mini-kidneys Successfully Grown from Stem Cells
Researchers from Murdoch Childrens Research Institute have perfected a method of turning stem cells into mini-kidneys for use in drug screening, disease modelling and cell therapy.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,700+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos