Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Products>This Product
  Products


Human Nanog Differentiation Reporter (pGreenZeo, plasmid)

Product Description
Accurately monitor the pluripotent state Mammalian development requires the specification of over 200 unique cell types from a single totipotent cell. Embryonic stem (ES) cells are derived from the inner cell mass of the developing blastocyst and can be propagated in culture in an undifferentiated state while maintaining the capacity to generate any cell type in the body. The recent derivation of human ES cells provides a unique opportunity to study early development and an understanding of the transcriptional regulatory circuitry that is responsible for pluripotency and self-renewal in human ES cells is fundamental to understanding human development and realizing the therapeutic potential of these cells. Three transcription factors are known to be critical in the maintenance of ESC pluripotency: Oct4, Nanog, and Sox2. Oct4 (Pou5f1) has a highly conserved role in maintaining pluripotent cell populations (Nichols et al., 1998; Morrison and Brickman, 2006), and its expression level dictates ESC fate (Niwa et al., 2000). SOX2 forms a complex with OCT4 and is necessary to cooperatively activate target genes in ESCs (Yuan et al., 1995; Ambrosetti et al., 1997). These factors comprise one essential circuit regulating ESC pluripotency in which OCT4 regulates Sox2, and additionally, the OCT4-SOX2 complex activates Oct4 expression (Okumura-Nakanishi et al., 2005). The transcription factors OCT4, SOX2, and NANOG have essential roles in early development and are required for the propagation of undifferentiated embryonic stem (ES) cells in culture. SBI has built lentivector-based transciptional Response Reporters that are responsive to Oct4 or Sox2 activity as response elements or Promoter Reporters for either Human or Mouse Oct4 and Nanog into the pGreenZeo, pRedZeo and pRedTK systems. Choice of fluorescent screening marker The lenti-reporter systems employ either a Green Fluorescent Protein (copGFP) or a Red Fluorescent Protein (RFP). Depending upon your screen, either Green or Red markers may be suitable and compatible with other fluorescent protein markers in your experimental scheme. The Response Reporters feature the dual reporting system with both GFP and Luciferase. Choice of antibiotic selection marker The Response Reporters have an optional constitutive EF1-Puro or -Neo selection cassette for establishing cells lines. The Promoter Reporters feature Zeomycin (Zeo) resistance marker confers resistance to the antibiotic Zeocin™. Zeocin™ causes cell death by intercalating into DNA and causes DNA strand breakage. This antibiotic is effective on most aerobic cells and is therefore useful for selection in bacteria, eukaryotic microorganisms, plant and animal cells. Thymidine kinase (TK) is a Herpes simplex virus-derived enzyme that acts on the guanosine nucleotide analog ganciclovir. TK phosphorylates ganciclovir, and TK+ cells produce highly-toxic triphosphates that lead to cell death (negative selection). All Stem Cell Pluipotency Monitors are available as lentivector plasmids or as pre-packaged lentiviral preparations (using the HIV lentiviral backbone and pseudotyped with VSV-G protein)—ready for transduction of target cells. The standard product size is 10 ug of plasmid or 1 x 10^6 IFUs per packaged lentiviral reporter. Positive and negative transduction control plasmids and pre-packaged viral particles are also available.
Product Human Nanog Differentiation Reporter (pGreenZeo, plasmid)
Company System Biosciences
Price Request a quote
More Information View company product page
Catalog Number SR10030PA-1
Quantity 10 ug
Company Logo

System Biosciences
265 North Whisman Rd. Mountain View, CA 94043, USA

Tel: 1.650-968-2200
Fax: 1.650.968.2277
Email: info@systembio.com



Scientific News
Platelets are the Pathfinders for Leukocyte Extravasation During Inflammation
Findings from the study could help in the prevention and treatment of inflammatory pathologies.
ASMS 2016: Targeting Mass Spectrometry Tools for the Masses
The expanding application range of MS in life sciences, food, energy, and health sciences research was highlighted at this year's ASMS meeting in San Antonio, Texas.
Benchtop Automation Trends
Gain a better understanding of current interest in and future deployment of benchtop automated systems.
Manufactured Stem Cells to Advance Clinical Research
Clinical-grade cell line will enable development of new therapies and accelerate early-stage clinical research.
Dengue Virus Exposure May Amplify Zika Infection
Researchers at Imperial College London have found that the previous exposure to the dengue virus may increase the potency of Zika infection.
Gender Determination in Forensic Investigations
This study investigated the effectiveness of lip print analysis as a tool in gender determination.
Identifying Novel Types of Forensic Markers in Degraded DNA
Scientists have tried to verify the nucleosome protection hypothesis by discovering STRs within nucleosome core regions, using whole genome sequencing.
Proteins in Blood of Heart Disease Patients May Predict Adverse Events
Nine-protein test shown superior to conventional assessments of risk.
Higher Frequency of Huntington's Disease Mutations Discovered
University of Aberdeen study shows that the gene change that causes Huntington's disease is much more common than previously thought.
Starving Stem Cells May Enable Scientists To Build Better Blood Vessels
Researchers from the University of Illinois at Chicago College of Medicine have uncovered how changes in metabolism of human embryonic stem cells help coax them to mature into specific cell types — and may improve their function in engineered organs or tissues.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,600+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!