Nonviral and non-integrating minicircle (mc) DNA technology available as well as a Human mc-iPS Cell Line
The minicircle DNA features easy-to-transfect molecules that have an extended expression lifespan in cells to efficiently reprogram somatic cells to the pluripotent state. After 21 days, the non-integrating mc-DNAs disappear and the iPS cells are fully reprogrammed. SBI offers the pre-made, ready-to-transfect 4-in-1 minicircle reprogramming DNA as well as the Human mc-iPS Cell line highlighted in Nature Methods, A nonviral minicircle vector for deriving human iPS cells. Jia F, et al., 2010 Mar;7(3):197-9. The mc-iPS Cell Line was derived from adult human adipose stem cells (hASCs).