Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Products>This Product
  Products


MaxDiscovery™ GAPDH ELISA Kit

Product Description
The MaxDiscovery™ Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) ELISA Kit is an enzyme immunoassay that analyzes the quantity of GAPDH in cells, tissues, serum or urine. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH initiates the second stage of glycolysis, catalyzing the reaction that converts glyceraldehyde 3-phosphate (GAP) into 1,3 bisphosphoglycerate (1,3 BPG). GAPDH oxidizes and phosphorylates GAP to produce 1,3 BPG, which is then used as an intermediate in the synthesis of ATP. While the glycolytic function of GAPDH is widely known, recent evidence suggests that GAPDH is a highly versatile molecule that plays several diverse roles in living systems. Mammalian GAPDH is involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. There have also been many findings that GAPDH plays a role in different pathologies including prostate cancer progression, programmed neuronal cell death and age-related neuronal diseases, i.e. Alzheimer’s and Huntington’s disease. The GAPDH gene is constitutively expressed at high levels in almost all tissues. However, the molecular mechanism that sustains high-level expression of this housekeeping enzyme is still unclear. GAPDH is almost always a tetramer and is localized to the cytoplasm in healthy cells. Translocation of GAPDH into the nucleus is seen during its role in the early stages of apoptosis and oxidative stress. Because of its high-level and constitutive expression, GAPDH is widely used as a loading control for Northern/Western blots and for protein normalization. Like most ELISA assays, the MaxDiscovery™ GAPDH ELISA Test relies on a Horseradish Perioxidase (HRP) conjugated antibody and the TMB (3,3´,5,5´-tetramethylbenzidine) substrate. TMB is a chromogen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) that has major absorbances at 370 nm and 652 nm. The color then changes to yellow with the addition of acid with maximum absorbance at 450 nm. The relative amount of GAPDH protein in the cells will be directly proportional to the amount of signal that is obtained at 450 nm. Selected Citations: Jones, J. A. et al. (July, 2010) Alterations in membrane type-1 matrix metalloproteinase abundance after the induction of thoracic aortic aneurysm in a murine model. Am J Physiol Heart Circ Physiol, 299: H114 - H124. doi: 10.1152/ajpheart.00028.2010
Product MaxDiscovery™ GAPDH ELISA Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 1 x 96 wells
Quantity 510
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122
Email: info@biooscientific.com



Scientific News
Point of Care Diagnostics - A Cautious Revolution
Advances in molecular biology, coupled with the miniaturization and improved sensitivity of assays and devices in general, have enabled a new wave of point-of-care (POC) or “bedside” diagnostics.
Mass Spec Technology Drives Innovation Across the Biopharma Workflow
With greater resolving power, analytical speed, and accuracy, new mass spectrometry technology and techniques are infiltrating the biopharmaceuticals workflow.
One Step Closer to Precision Medicine for Chronic Lung Disease Sufferers
A study led by University of North Carolina at Chapel Hill, and National Jewish Health, has provided evidence of links between SNPs and known COPD blood protein biomarkers.
Blood Pressure Drug May Boost Effectiveness of Lung Cancer Treatment
Researchers at Imperial College London have suggested that the blood pressure drug may make a type of lung cancer treatment more effective.
Insight into Eye Diseases
Scientists recreate zebrafish cell regeneration from retinal stem cells in mice.
New Discovery May Benefit Farmers Worldwide
Scientists have shown how a crop-microbe 'team' protect against fungal infection.
Antibodies Paving the Way to HIV Vaccine
Researchers uncover factors responsible for the formation of broadly neutralizing HIV antibodies in humans.
Designing Drugs with a Whole New Toolbox
Researchers develop methods to design small, targeted proteins with shapes not found in nature.
Protein Studies Discover Molecular Secrets
Two protein studies have mapped proteins that reveal the secrets to recycling carbon and healing cells.
Tapping Evolution to Improve Biotech Products
Researchers show how 'ancestral sequence reconstruction' can be used to guide engineering of a blood clotting protein.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!