Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Products>This Product
  Products


MaxDiscovery™ GAPDH ELISA Kit

Product Description
The MaxDiscovery™ Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) ELISA Kit is an enzyme immunoassay that analyzes the quantity of GAPDH in cells, tissues, serum or urine. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH initiates the second stage of glycolysis, catalyzing the reaction that converts glyceraldehyde 3-phosphate (GAP) into 1,3 bisphosphoglycerate (1,3 BPG). GAPDH oxidizes and phosphorylates GAP to produce 1,3 BPG, which is then used as an intermediate in the synthesis of ATP. While the glycolytic function of GAPDH is widely known, recent evidence suggests that GAPDH is a highly versatile molecule that plays several diverse roles in living systems. Mammalian GAPDH is involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. There have also been many findings that GAPDH plays a role in different pathologies including prostate cancer progression, programmed neuronal cell death and age-related neuronal diseases, i.e. Alzheimer’s and Huntington’s disease. The GAPDH gene is constitutively expressed at high levels in almost all tissues. However, the molecular mechanism that sustains high-level expression of this housekeeping enzyme is still unclear. GAPDH is almost always a tetramer and is localized to the cytoplasm in healthy cells. Translocation of GAPDH into the nucleus is seen during its role in the early stages of apoptosis and oxidative stress. Because of its high-level and constitutive expression, GAPDH is widely used as a loading control for Northern/Western blots and for protein normalization. Like most ELISA assays, the MaxDiscovery™ GAPDH ELISA Test relies on a Horseradish Perioxidase (HRP) conjugated antibody and the TMB (3,3´,5,5´-tetramethylbenzidine) substrate. TMB is a chromogen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) that has major absorbances at 370 nm and 652 nm. The color then changes to yellow with the addition of acid with maximum absorbance at 450 nm. The relative amount of GAPDH protein in the cells will be directly proportional to the amount of signal that is obtained at 450 nm. Selected Citations: Jones, J. A. et al. (July, 2010) Alterations in membrane type-1 matrix metalloproteinase abundance after the induction of thoracic aortic aneurysm in a murine model. Am J Physiol Heart Circ Physiol, 299: H114 - H124. doi: 10.1152/ajpheart.00028.2010
Product MaxDiscovery™ GAPDH ELISA Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 1 x 96 wells
Quantity 510
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122
Email: info@biooscientific.com



Scientific News
The Changing Tides of the In Vitro Diagnostics Market
With the increasing focus in personalized medicine, diagnostics plays a crucial role in patient monitoring.
LaVision BioTec Reports on the Neuro Research on the Human Brain After Trauma
Company reports on the work of Dr Ali Ertürk from the Institute for Stroke and Dementia Research at LMU Munich.
NIH Study Shows No Benefit of Omega-3 Supplements for Cognitive Decline
Research was published in the Journal of the American Medical Association.
Less May Be More in Slowing Cholera Epidemics
Mathematical model shows more cases may be prevented and more lives saved when using one dose of cholera vaccine instead of recommended two doses.
Investigating the Vape
Expert independent review concludes that e-cigarettes have potential to help smokers quit.
NIH Launches Human RSV Study
Study aims to understand infection in healthy adults to aid development of RSV medicines, vaccines.
Researchers Discover Synthesis of a New Nanomaterial
Interdisciplinary team creates biocomposite for first time using physiological conditions.
Poor Survival Rates in Leukemia Linked to Persistent Genetic Mutations
For patients with an often-deadly form of leukemia, new research suggests that lingering cancer-related mutations – detected after initial treatment with chemotherapy – are associated with an increased risk of relapse and poor survival.
Flu Remedies Help Combat E. coli Bacteria
Physiologists from the University of Zurich have now discovered why the intestinal bacterium Escherichia coli (E. coli) multiplies heavily and has an inflammatory effect.
Marijuana Genome Unraveled
A study by Canadian researchers is providing a clearer picture of the evolutionary history and genetic organization of cannabis, a step that could have agricultural, medical and legal implications for this valuable crop.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!