Corporate Banner
Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>Products>This Product

MaxDiscovery™ Luciferase ELISA Kit

Product Description
The MaxDiscovery™ Luciferase ELISA Kit is an enzyme immunoassay that analyzes the quantity of Luciferase in cells, serum and tissues. Luciferase is a generic name for enzymes, commonly found in nature, that produce light. The most commonly used Luciferase is produced by, Photinus pyralis, the American firefly, which can catalyze a chemiluminescent oxidation–reduction reaction. Although many kits are available to detect the enzymatic activity of this protein using light emission, these methods have several drawbacks. For example, these assays have rapid decay kinetics and require the enzymatic activity of the Luciferase to be retained. Since enzyme activity can be influenced by many factors including handling, storage and buffer conditions, these assays are more variable and more difficult to reproduce than ELISA based assays. In addition, enzyme based activity assays do not easily enable absolute quantification as enzyme activity is relative to a standard that does not contain equal representative activity with respect to the quantity of luciferase. In response to these issues, Bioo Scientific has developed a highly sensitive ELISA assay which allows the researcher to completely measure the amount of firefly Luciferase protein from a number of sources. Like most ELISA assays, the MaxDiscovery™ Luciferase ELISA kit relies on a Horseradish Peroxidase (HRP) conjugated Luciferase antibody and the TMB (3,3´,5,5´-tetramethylbenzidine) substrate. TMB is a chromogen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) and has major absorbances at 370 nm and 652 nm. The color then changes to yellow with the addition of acid with maximum absorbance at 450 nm. The relative amount of Luciferase protein in the cells will be directly proportional to the amount of signal that is obtained at 450 nm.
Product MaxDiscovery™ Luciferase ELISA Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 1 x 96 wells
Quantity 510
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122

Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
How a Genetic Locus Protects Adult Blood-Forming Stem Cells
Mammalian imprinted Gtl2 protects adult hematopoietic stem cells by restricting metabolic activity in the cells' mitochondria.
Genetic Basis of Fatal Flu Side Effect Discovered
A group of people with fatal H1N1 flu died after their viral infections triggered a deadly hyperinflammatory disorder in susceptible individuals with gene mutations linked to the overactive immune response, according to a recent study.
New Tech Vastly Improves CRISPR/Cas9 Accuracy
A new CRISPR/Cas9 technology developed by scientists at UMass Medical School is precise enough to surgically edit DNA at nearly any genomic location, while avoiding potentially harmful off-target changes typically seen in standard CRISPR gene editing techniques.
The MaxSignal Colistin ELISA Test Kit from Bioo Scientific
Kit can help prevent the antibiotic apocalypse by keeping last resort drugs out of the food supply.
"Good" Mozzie Virus Might Hold Key to Fighting Human Disease
Australian scientists have discovered a new virus carried by one of the country’s most common pest mosquitoes.
Non-Disease Proteins Kill Brain Cells
Scientists at the forefront of cutting-edge research into neurodegenerative diseases such as Alzheimer’s and Parkinson’s have shown that the mere presence of protein aggregates may be as important as their form and identity in inducing cell death in brain tissue.
Closing the Loop on an HIV Escape Mechanism
Research team finds that protein motions regulate virus infectivity.
New Class of RNA Tumor Suppressors Identified
Two short, “housekeeping” RNA molecules block cancer growth by binding to an important cancer-associated protein called KRAS. More than a quarter of all human cancers are missing these RNAs.
Potential Treatment for Life-Threatening Viral Infections Revealed
The findings point to new therapies for Dengue, West Nile and Ebola.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos