Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Products>This Product
  Products


MaxDiscovery™ Human IL-12 ELISA Test Kit

Product Description
The MaxDiscovery™ Human IL-12 ELISA Test Kit is designed for quantitative determination of the concentration of bioactive, heteromeric form of human IL-12, p70, without interference by p40 monomer, homodimer, or IL-23 (p19/p40) in serum, plasma, and cell culture supernatant. Human interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) and cytotoxic lymphocyte maturation factor (CLMFIL-12), is a 75kDa glycoprotein heterodimer composed of two unequal, genetically-unrelated subunits. The smaller subunit (p35) and the larger subunit (P40). Each subunit of IL-12 apparently arises from a single copy gene. The transcription of the subunit of the subunit mRNAs is closely co-ordinated, although an excess of the larger subunit (P40) has been shown to be produced by B cells in addition to active IL-12. Expression of the smaller chain (p35) is reported to be enhanced by simultaneous expression of the larger chain (p40). Although IL-12 activity cannot be demonstrated in the absence of either chain, the presence of only p40 is associated with inhibition of IL-12-associated activities. As suggested by their names, p35 has a native molecular weight of 35 kDa while p40 has a native molecular weight of 40 kDa. In humans, p35 is a 22.5 kDa protein of 197 amino acids containing 7 cysteine residues plus 3 potential N-glycosylation sites and the molecule is believed to be heavily glycosylated. The p40 subunit is a 34.7 kDa protein of 306 amino acids containing 10 cysteine residues and four potential N-linked glycosylation sites. The murine p35 and p40 subunits show 60% sequence identity and 70% sequence identity to human p35 and p40, respectively. IL-12 is a pleitopic cytokine originally identified in the medium of cultured EBV-transformed RPMI-8866 cells. IL-12 is produced by monocytes/macrophages, B cells and connective tissue type mast cells. IL-12 shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.
Product MaxDiscovery™ Human IL-12 ELISA Test Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 2120-01
Quantity 1 x 96 wells
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122
Email: info@biooscientific.com



Scientific News
Sorting Through Cellular Statistics
Aaron Dinner, professor in chemistry, and his graduate student Herman Gudjonson are trying to read the manual of life, DNA, as part of the Dinner group’s research into bioinformatics—the application of statistics to biological research.
Playing 'Tag' with Pollution lets Scientists See Who's It
Using a climate model that can tag sources of soot from different global regions and can track where it lands on the Tibetan Plateau, researchers have determined which areas around the plateau contribute the most soot — and where.
Women’s Immune System Genes Operate Differently from Men’s
A new technology reveals that immune system genes switch on and off differently in women and men, and the source of that variation is not primarily in the DNA.
Long Telomeres Associated with Increased Lung Cancer Risk
Genetic predisposition for long telomeres predicts increased lung adenocarcinoma risk.
First Artificial Ribosome Designed
Researchers at the University of Illinois at Chicago and Northwestern University have engineered a tethered ribosome that works nearly as well as the authentic cellular component, or organelle, that produces all the proteins and enzymes within the cell.
High-Resolution 3D Images Reveal the Muscle Mitochondrial Power Grid
NIH mouse study overturns scientific ideas on energy distribution in muscle.
Expanding the Brain
A team of researchers has identified more than 40 new “imprinted” genes, in which either the maternal or paternal copy of a gene is expressed while the other is silenced.
Identifying a Key Growth Factor in Cell Proliferation
Researchers discover that aspartate is a limiter of cell proliferation.
Study Uncovers Target for Preventing Huntington’s Disease
Scientists from Cardiff University believe that a treatment to prevent or delay the symptoms of Huntington’s disease could now be much closer, following a major breakthrough.
Paving the way to Better Ovarian Cancer Diagnosis
Aïcha BenTaieb will present her invention for automated identification of ovarian cancer’s many subtypes at an international conference this fall.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!