Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Products>This Product
  Products


MaxDiscovery™ Aspartate Transaminase (AST) Cytotoxicity Assay Kit

Product Description
The MaxDiscovery™ Aspartate Transaminase (AST) Cytotoxicity Kit is a plate-based colorimetric enzymatic assay for the determination of the aspartate transaminase enzyme in cultured cell lines. Aspartate transaminase (AST) also known as aspartate aminotransferase or (sGOT) is a metabolic enzyme expressed in a host of mammalian cell types. Upon cell damage or lysis the enzyme is released into the cell culture supernatant and is thus a useful tool for the investigation of cytotoxicity effects of experimental drug formulations and cell transfection. The MaxDiscovery™ Alanine Transaminase (AST) Cytotoxicity Assay Kit uses a coupled enzymatic reaction scheme: aspartate and a-ketoglutarate are first converted to glutamate and oxaloacetate which is converted by malate dehydrogenase to make malate and NAD+. The conversion of the NADH chromophore to NAD+ product, measured at 340 nm, is proportional to the level of AST enzyme in the sample. The absorbance of each well at 340 nm is measured using a plate reader. The concentration of AST in each sample is then directly determined from the change in absorbance at 340 nm over a 30 minute assay length. Although AST is present in a host of human tissues, its release varies among cell types. Therefore, the most useful analysis of cytotoxicity in a cultured cell line involves measuring cytotoxicity as a percentage of damaged or lysed cells.
Product MaxDiscovery™ Aspartate Transaminase (AST) Cytotoxicity Assay Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 3460-11
Quantity 192 rxns
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122
Email: info@biooscientific.com



Scientific News
Releasing Cancer Cells for Better Analysis
A new device developed at the University of Michigan could provide a non-invasive way to monitor the progress of an advanced cancer treatment.
Releasing Cancer Cells for Better Analysis
A new device developed at the University of Michigan could provide a non-invasive way to monitor the progress of an advanced cancer treatment.
Apricot Kernels Pose Risk of Cyanide Poisoning
Eating more than three small raw apricot kernels, or less than half of one large kernel, in a serving can exceed safe levels. Toddlers consuming even one small apricot kernel risk being over the safe level.
Cell Transplant Treats Parkinson’s in Mice
A University of Wisconsin—Madison neuroscientist has inserted a genetic switch into nerve cells so a patient can alter their activity by taking designer drugs that would not affect any other cell.
Understanding Female HIV Transmission
Glowing virus maps points of entry through entire female reproductive tract for first time.
Genetic Markers Influence Addiction
Differences in vulnerability to cocaine addiction and relapse linked to both inherited traits and epigenetics, U-M researchers find.
Lab-on-a-Chip for Detecting Glucose
By integrating microfluidic chips with fiber optic biosensors, researchers in China are creating ultrasensitive lab-on-a-chip devices to detect glucose levels.
A lncRNA Regulates Repair of DNA Breaks in Breast Cancer Cells
Findings give "new insight" into biology of tough-to-treat breast cancer.
COPD Linked to Increased Bacterial Invasion
Persistent inflammation in COPD may result from a defect in the immune system that allows airway bacteria to invade deeper into the lung.
Detection of HPV in First-Void Urine
Similar sensitivity of HPV test on first void urine sample compared to cervical smear.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,400+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!