The optimal reaction conditions of a PCR experiment depend on the template DNA and the respective primer pair. The main parameters that may vary between different applications are the magnesium concentration, pH of the reaction buffer, and the temperature and time of the different cycling steps. Additionally, various additives may increase the specificity and yield of some applications. Therefore, it is generally recommended to optimize PCR conditions for each new template/primer pair. However, the preparation of the various solutions is time-consuming and may increase the risk of contamination. The PCR Optimization Kit is the convenient and reliable solution for optimizing your PCR experiments. It contains a set of buffers with different pH and magnesium concentrations spanning the most-often-used reaction conditions. Four different additives allow additional optimization to increase yield and specificity.