Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Products>This Product
  Products


5’/3’ RACE Kit, 2nd Generation

Product Description
Robust and reliable amplification of long unknown sequence by RACE.
Obtaining a full-length cDNA is of critical importance for structural and expression studies. The amplification of DNA sequences from a messenger RNA template between a defined internal site and unknown sequences of either the 3' or the 5' end of the mRNA are often referred to as RACE (rapid amplification of cDNA ends), “anchored” PCR, or “one-sided” PCR. Transcriptor Reverse Transcriptase is supplied with the kit to transcribe full-length cDNA up to 14 kb in length and, due to the thermostability of Transcriptor Reverse Transcriptase (up to 65°C), to work with GC-rich templates with high secondary structure. In addition, with Transcriptor Reverse Transcriptase, high sensitivity can be achieved. This results in highly efficient cDNA synthesis and the generation of long RACE products.
Purification of the first-strand cDNA is performed with the High Pure PCR Product Purification Kit. Recombinant Terminal Transferase is used to add a homopolymeric A-tail to the 3' end of the cDNA. The use of poly(A) tail decreases the likelihood of inappropriate trunction by the oligo dT-anchor primer, and due to the weaker A/T binding compared to the G/C binding, longer stretches of A residues are required before the oligo dT-anchor primer will bind to an internal site and truncate the amplification product.
Tailed cDNA is amplified by PCR using a gene-specific primer and the oligo dT-anchor primer. The obtained cDNA is further amplified by a second PCR using a nested specific primer and the PCR anchor primer. As a result, the RACE products can be cloned into an appropriate vector for subsequent characterization studies.
Product 5’/3’ RACE Kit, 2nd Generation
Company Roche - Products Listing
Price Request a quote
More Information View company product page
Catalog Number 03353621001
Quantity 1 kit (10 reactions)
Company Logo

Roche - Products Listing
Roche Diagnostics Corporation Roche Applied Science P.O. Box 50414 9115 Hague Road Indianapolis, IN 46250-0414 US

Tel: +1-800-428-5433
Fax: +1-800-428-2883



Scientific News
How Skeletal Stem Cells Form The Blueprint Of The Face
USC researchers discover that two types of molecular signals work to control where and when stem cells turn into facial cartilage.
Intestinal Worms Boost Immune System In A Surprising Way
EPFL researchers find that intestinal worm infections cause lymph nodes to produce more immune cells as well as grow in size.
Measuring The Airborne Toxicants Urban Bicyclists Inhale
Researchers analyze breath biomarkers to measure uptake of volatile organic compounds by bicyclists.
Breast Milk Hormones Impact Bacteria In Infants’ Guts
Intestinal microbiome of children born to obese mothers significantly different from those born to mothers of healthy weight, CU Anschutz researchers find.
Newborn Screening Test Developed For Rare, Deadly Neurological Disorder
Scientists have developed a new dried blood spot screening test for Niemann-Pick type C, with goal to speed diagnosis and treatment.
'Kidney on a Chip' Facilitates Safer Drug Dosing
University of Michigan researchers have used a "kidney on a chip" device to mimic the flow of medication through human kidneys and measure its effect on kidney cells.
New Autism Blood Biomarker Identified
Researchers at UT Southwestern Medical Center have identified a blood biomarker that may aid in earlier diagnosis of children with autism spectrum disorder, or ASD.
New Method Allows First Look At Embryo Implantation
Researchers at The Rockefeller University develop a method that shows the molecular and cellular processes that occur up to day 14 after fertilization.
Ketamine Metabolism Lifts Depression
NIH-funded team finds rapid-acting, non-addicting agent in mouse study.
Faster, Cheaper Way to Produce New Antibiotics
A novel way of synthesising a promising new antibiotic has been identified by scientists at the University of Bristol.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!