Corporate Banner
Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>Products>This Product

Shrimp WSSV Detection Test Kit

Product Description
The MaxSignal® Shrimp WSSV Detection Kit uses an end-point Polymerase Chain Reaction (PCR) assay to screen for WSSV in fresh or frozen shrimp or other potentially infectious material. White Spot Syndrome Virus (WSSV) is claimed to be the most serious viral pathogen that affects cultivated shrimp worldwide. The MaxSignal® Shrimp WSSV Detection Kit contains 2 pairs of oligonucleotide primers targeting nested regions of the WSSV VP466 gene (accession # EF564812), which encodes a structural protein located in the nucleocapsid. The WSSV amplicon produced using the WSSV-483 primers in the kit is 483 bp and the amplicon produced using the WSSV-388 primers is 388 bp. These WSSV amplicons have no significant homology to any other viral sequence. The detection limit for the synthetic WSSV template included in the kit is approximately 50 copies using the standard 1-step PCR protocol, and approximately 5 copies using the 2-step nested PCR protocol. For detection of WSSV in infected shrimp, positive signals have been obtained using the 1-step PCR protocol with input amounts of approximately 50 femtograms (50 x 10-15 gm) of total nucleic acid extracted from shrimp that were experimentally infected with high levels of the WSSV virus. The Shrimp Positive Control primers included in the kit were designed to the actin 1 gene (Accession # AF100986) from Penaeus monodon and will produce an amplicon of 249 bp from P monodon and also from Penaeus vannamei. The Shrimp Positive Control primers are used to demonstrate successful extraction of DNA that is able to support PCR. Input sample for this kit is total DNA or total nucleic acid extracted from solid tissue or derived from liquid tissues such as hemolymph; purification of viral particles for analysis is not required. To maximize user convenience, the reagents for PCR (buffer, dNTPs, and thermostable polymerase) are provided as a 5x MasterMix. The MasterMix also contains components to allow the PCR to be directly loaded for gel analysis, without adding gel-loading solution.
Product Shrimp WSSV Detection Test Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 1201
Quantity 100 PCR rxns
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122

Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
How a Genetic Locus Protects Adult Blood-Forming Stem Cells
Mammalian imprinted Gtl2 protects adult hematopoietic stem cells by restricting metabolic activity in the cells' mitochondria.
Genetic Basis of Fatal Flu Side Effect Discovered
A group of people with fatal H1N1 flu died after their viral infections triggered a deadly hyperinflammatory disorder in susceptible individuals with gene mutations linked to the overactive immune response, according to a recent study.
New Tech Vastly Improves CRISPR/Cas9 Accuracy
A new CRISPR/Cas9 technology developed by scientists at UMass Medical School is precise enough to surgically edit DNA at nearly any genomic location, while avoiding potentially harmful off-target changes typically seen in standard CRISPR gene editing techniques.
The MaxSignal Colistin ELISA Test Kit from Bioo Scientific
Kit can help prevent the antibiotic apocalypse by keeping last resort drugs out of the food supply.
"Good" Mozzie Virus Might Hold Key to Fighting Human Disease
Australian scientists have discovered a new virus carried by one of the country’s most common pest mosquitoes.
Non-Disease Proteins Kill Brain Cells
Scientists at the forefront of cutting-edge research into neurodegenerative diseases such as Alzheimer’s and Parkinson’s have shown that the mere presence of protein aggregates may be as important as their form and identity in inducing cell death in brain tissue.
Closing the Loop on an HIV Escape Mechanism
Research team finds that protein motions regulate virus infectivity.
New Class of RNA Tumor Suppressors Identified
Two short, “housekeeping” RNA molecules block cancer growth by binding to an important cancer-associated protein called KRAS. More than a quarter of all human cancers are missing these RNAs.
Potential Treatment for Life-Threatening Viral Infections Revealed
The findings point to new therapies for Dengue, West Nile and Ebola.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos