Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Products>This Product
  Products


Listeria Extraction and Detection Test Kit

Product Description
The MaxSignal® Listeria Extraction and Detection Kit uses an end-point PCR assay to screen for the presence of Listeria bacteria in potentially infectious material. The purification method is based on lysis of Listeria pellets at high temperature followed by solid-phase extraction of the DNA on silica filters. The procedure can be carried out in less than 30 min using common laboratory equipment. The use of purified Listeria DNA allows for increased sensitivity compared to Listeria detection methods that use samples consisting of unpurified bacterial lysates, because the DNA is concentrated into a smaller volume for use in volume-limited PCR reactions. The kit also contains 3 synthetic templates for use as Internal Amplification Controls. The synthetic templates have primer binding sites for one of the Outer Primer Pairs (A, B, or C) and an internal region consisting of a plasmid DNA sequence. The plasmid sequence is the same in all 3 templates. The control templates are typically added to the PCRs along with the sample DNA, to create positive amplification controls. Amplification of the expected size amplicons from the synthetic templates proves that the sample DNA did not contain inhibitors of PCR. To maximize user convenience, the reagents for PCR (buffer, dNTPs, and thermostable polymerase) are provided as a 5x MasterMix. The MasterMix also contains components to allow the PCR to be directly loaded for gel analysis, without adding gel-loading solution. The kit includes a molecular weight “ladder” for analysis of results from experimental samples. The kit contains 6 pairs of oligonucleotide primers targeting outer and nested regions of three different types of Listeria sample: 2 primer pairs specific for all Listeria species (Primer Pairs A outer and A nested); 2 primer pairs specific for Listeria monocytogenes (Primer Pairs B outer and B nested); and 2 primer pairs specific for Listerial moncytogenes isolate 4b (Primer Pairs C outer and C nested). The primers were designed against Listeria target genes. The kit also includes reagents and materials for purification of Listeria DNA from liquid cultures.

Product Listeria Extraction and Detection Test Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 1202
Quantity 50 Extractions & 100 PCR rxns
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122
Email: info@biooscientific.com



Scientific News
Big Genetics in BC: The American Society for Human Genetics 2016 Meeting
Themes at this year's meeting ranged from the verification, validation, and sharing of data, to the translation of laboratory findings into actionable clinical results.
Stem Cells in Drug Discovery
Potential Source of Unlimited Human Test Cells, but Roadblocks Remain.
Automated Low Volume Dispensing Trends
Gain a better understanding of the current and future market requirements for fully automated LVD systems.
Forensic 3D Documentation of Skin Injuries
In this study, the validity of using photogrammetry for documenting injuries in a pathological context was demonstrated.
3-D Printed Dog’s Nose Improves Vapor Detection
By mimicking how dogs get their whiffs, a team of government and university researchers have demonstrated that “active sniffing” can improve by more than 10 times the performance of current technologies that rely on continuous suction to detect trace amounts of explosives and other contraband.
New Markers for Forensic Body-fluid Identification
University of Bonn researchers have successfully identified specific Micro-RNA signatures to help forensically identify body fluids.
Genetics Control Regenerative Properties Of Stem Cells
Researchers define how genetic factors control regenerative properties of blood-forming stem cells.
Major Neuroscience Initiative Launched
Tianqiao and Chrissy Chen Institute invest $115 million to further expand neuroscience research, while Caltech construct $200 million biosciences complex.
Making It Personal
Cancer vaccine linked to increased immune response against leukemia cells.
Unique Visual Stimulation May Be New Treatment for Alzheimer’s
Noninvasive technique reduces beta amyloid plaques in mouse models of Alzheimer’s disease.
Scroll Up
Scroll Down

Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
4,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,300+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!