The MaxDiscovery™ Anti-Mouse Western Blot Kit is a complete kit that contains all necessary reagents to perform a western blot when a mouse secondary antibody is required. Western blots are used to detect specific proteins that have been separated from one another according to their size by gel electrophoresis. Detection involves a transfer procedure followed by probing the blot membrane with a primary antibody that recognizes specific amino acids on your protein of interest. A secondary antibody conjugated with peroxidase is used to bind with the primary antibody. To detect this, a chemiluminescent substrate is added to react with the antibody complex, revealing the location and relative amount of protein.