The ExoMir™ Kit is designed for the fractionation of, and RNA extraction from, exosomes and other microparticles in cell-free fluids. The ExoMir Kit uses a rapid fractionation approach in which samples are passed over syringe filters to capture exosomes and larger membrane-bound particles. The filters are then flushed with an RNA extraction reagent to lyse the captured particles and release their contents. In the standard procedure, samples are passed over 2 filters connected in series, to effectively capture particles such as apoptotic bodies, microvesicles on the upper filter and exosomes on the lower filter. Then the filters are disconnected and separately flushed with BiooPure™-MP to lyse the captured particles and release their contents. BiooPure-MP is a single-phase RNA extraction reagent containing guanidinium thiocyanate and phenol, which has been optimized to provide maximal recovery of the low-mass amounts of RNA in microparticles. Recovery of RNA is further improved by using the inert co-precipitant (linear acrylamide) included in the kit. Cell-free fluids that can be processed with the ExoMir™ Kit include blood serum, cerebrospinal fluid, and eukaryotic cell culture media (“conditioned media”). Recent research has highlighted the role of small membrane-bound particles such as exosomes and microvesicles, as intercellular mediators of biological information. Traditional methods for recovering exosomes involve centrifuging liquid samples at increasing centrifugal force, to sequentially pellet the larger and smaller particles. Traditionally to recover exosomes, ultracentrifugation of samples at relative centrifugal force of at least 100,000 x g for several hours or more is required.
Retractable Protein Nanoneedles The ability to control the transfer of molecules through cellular membranes is an important function in synthetic biology; a new study from researchers at Harvard’s Wyss Institute for Biologically Inspired Engineering and Harvard Medical School (HMS) introduces a novel mechanical method for controlling release of molecules inside cells.Leukemia’s Surroundings Key to its Growth Researchers at The University of Texas at Austin have discovered that a type of cancer found primarily in children can grow only when signaled to do so by other nearby cells that are noncancerous.Common Cell Transformed into Master Heart Cell By genetically reprogramming the most common type of cell in mammalian connective tissue, researchers at the University of Wisconsin—Madison have generated master heart cells — primitive progenitors that form the developing heart.‘Smelling’ Prostate Cancer A research team from the University of Liverpool and the University of the West of England (UWE Bristol) has reached an important milestone towards creating a urine diagnostic test for prostate cancer that could mean that invasive diagnostic procedures that men currently undergo eventually become a thing of the past.Genetic Mutation that Prevents Diabetes Complications The most significant complications of diabetes include diabetic retinal disease, or retinopathy, and diabetic kidney disease, or nephropathy. Both involve damaged capillaries.A Crystal Clear View of Biomolecules Fundamental discovery triggers paradigm shift in crystallography.Could the Food we Eat Affect Our Genes? Almost all of our genes may be influenced by the food we eat, according to new research.NIH Seeks Research Applications to Study Zika in Pregnancy, Developing Fetus Institute has announced that the new effort seeks to understand virus effect on reproduction and child development.Iron in the Blood Could Cause Cell Damage Concentrations of iron similar to those delivered through standard treatments can trigger DNA damage within 10 minutes, when given to cells in the laboratory. Neanderthal DNA Influences Human Disease Risk Large-scale, evolutionary analysis compares genetic data alongside electronic health records.