Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Products>This Product
  Products


MiraZol™ Small RNA Isolation Kit

Product Description
The MiraZol™ Small RNA Isolation Kit includes MiraZol RNA extraction reagent and other components for enrichment of small RNA from solid tissues and cells. Mirazol is a single-phase reagent that contains guanidinium, a powerful chaotropic agent effective for rapidly inactivating nucleases, and phenol, an organic solvent used to denature and separate proteins and DNA from RNA. The reagent recovers RNAs of approximately 100 bases and smaller, with less than ~5% by mass of contamination with larger RNAs. Solid tissues or cells are disrupted by grinding or vortexing in the MiraZol Reagent. The resulting lysate is mixed with 1-bromo-3-chloropropane (BCP) and centrifuged, which results in phase separation with the RNA partitioned in the upper aqueous phase. The large RNA is then removed by precipitation with low concentration of isopropanol. The supernatant is recovered and mixed with an inert co-precipitant and with additional isopropanol to precipitate the small RNA, which is then recovered by centrifugation. The pellet is washed with 75% ethanol and resuspended in RNase-free water containing a trace amount of EDTA, or in other solvent of choice.
Product MiraZol™ Small RNA Isolation Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 5302-01
Quantity up to 30 Preps
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122
Email: info@biooscientific.com



Scientific News
Criminal Justice Alcohol Program Linked to Decreased Mortality
Institute has announced that in the criminal justice alcohol program deaths dropped by 4.2 percent over six years.
Charting Kidney Cancer Metabolism
Changes in cell metabolism are increasingly recognized as an important way tumors develop and progress, yet these changes are hard to measure and interpret. A new tool designed by MSK scientists allows users to identify metabolic changes in kidney cancer tumors that may one day be targets for therapy.
Improving Regenerative Medicine
Lab-created stem cells may lack key characteristics, UCLA research finds.
Tick Genome Reveals Secrets of a Successful Bloodsucker
NIH has announced that decipher the genome of the blacklegged tick which could lead to new tick control methods.
"Dark Side" of the Transcriptome
New approach to quantifying gene "read-outs" reveals important variations in protein synthesis and has implications for understanding neurodegenerative diseases.
Individuals' Medical Histories Predicted by their Noncoding Genomes
Researchers have found that analyzing mutations in regions of the genome that control genes can predict medical conditions such as hypertension, narcolepsy and heart problems.
'Molecular Movie' Opens Door to New Cancer Treatments
An international team of scientists led by the University of Liverpool has produced a 'structural movie' revealing the step-by-step creation of an important naturally occurring chemical in the body that plays a role in some cancers.
New Source of Mutations in Cancer
Recently, a new mutation signature found in cancer cells was suspected to have been created by a family of enzymes found in human cells called the APOBEC3 family.
Advancing Synthetic Biology
Living systems rely on a dizzying variety of chemical reactions essential to development and survival. Most of these involve a specialized class of protein molecules — the enzymes.
Madison Researchers Begin Work on Zika Virus
Work will start with basic questions about Zika virus infection.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!