Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Products>This Product
  Products


ALL-TAIL™ Kit

Product Description
The patent pending technology of the ALL-TAIL™ Kit for extreme 3’ RACE, can be used to investigate the dynamic changes in poly(A) tail length that take place during gene expression, as well as the changes in 3’ untranslated regions caused by differential poly(A) tail addition site selection in development, differentiation and cancer. This kit enables the precise measurement of 3’ poly(A) tail length along with the ability to identify and characterize the sequence the 3’ end of RNA transcripts. It can also be used to detect miRNA molecules using a linker ligation technique followed by PCR. Bioo Scientific’s ALL-TAIL Kit provides a sensitive, accurate and highly reproducible assay to measure the poly(A) tail length and the 3’ UTR sequence of any RNA species. First, AIR™ Ligase (a highly efficient truncated T4 RNA ligase 2) efficiently joins an adenylated adaptor oligonucleotide to the 3’ end of the total RNAs. Next reverse transcription is performed using a primer specific to the adenylated adaptor to initiate the production of cDNA. Then a primer designed near the 3’ end of the transcript of interest is used along with the primer specific to the adenylated adaptor to perform PCR amplification of the poly(A) tail and a short stretch of the 3’ UTR. This step may be modified to amplify specific miRNA sequences. Finally, the PCR products are separated on an acrylamide gel and visualized using either SYBR Gold Nucleic Acid Stain or ethidium bromide.
Product ALL-TAIL™ Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 5205
Quantity 20 Ligations, 30 RT Rxns, 30 PCR Rxns
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122
Email: info@biooscientific.com



Scientific News
The Changing Tides of the In Vitro Diagnostics Market
With the increasing focus in personalized medicine, diagnostics plays a crucial role in patient monitoring.
LaVision BioTec Reports on the Neuro Research on the Human Brain After Trauma
Company reports on the work of Dr Ali Ertürk from the Institute for Stroke and Dementia Research at LMU Munich.
NIH Study Shows No Benefit of Omega-3 Supplements for Cognitive Decline
Research was published in the Journal of the American Medical Association.
Less May Be More in Slowing Cholera Epidemics
Mathematical model shows more cases may be prevented and more lives saved when using one dose of cholera vaccine instead of recommended two doses.
Investigating the Vape
Expert independent review concludes that e-cigarettes have potential to help smokers quit.
NIH Launches Human RSV Study
Study aims to understand infection in healthy adults to aid development of RSV medicines, vaccines.
Researchers Discover Synthesis of a New Nanomaterial
Interdisciplinary team creates biocomposite for first time using physiological conditions.
Poor Survival Rates in Leukemia Linked to Persistent Genetic Mutations
For patients with an often-deadly form of leukemia, new research suggests that lingering cancer-related mutations – detected after initial treatment with chemotherapy – are associated with an increased risk of relapse and poor survival.
Flu Remedies Help Combat E. coli Bacteria
Physiologists from the University of Zurich have now discovered why the intestinal bacterium Escherichia coli (E. coli) multiplies heavily and has an inflammatory effect.
Marijuana Genome Unraveled
A study by Canadian researchers is providing a clearer picture of the evolutionary history and genetic organization of cannabis, a step that could have agricultural, medical and legal implications for this valuable crop.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!