Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Products>This Product
  Products


ALL-TAIL™ Kit

Product Description
The patent pending technology of the ALL-TAIL™ Kit for extreme 3’ RACE, can be used to investigate the dynamic changes in poly(A) tail length that take place during gene expression, as well as the changes in 3’ untranslated regions caused by differential poly(A) tail addition site selection in development, differentiation and cancer. This kit enables the precise measurement of 3’ poly(A) tail length along with the ability to identify and characterize the sequence the 3’ end of RNA transcripts. It can also be used to detect miRNA molecules using a linker ligation technique followed by PCR. Bioo Scientific’s ALL-TAIL Kit provides a sensitive, accurate and highly reproducible assay to measure the poly(A) tail length and the 3’ UTR sequence of any RNA species. First, AIR™ Ligase (a highly efficient truncated T4 RNA ligase 2) efficiently joins an adenylated adaptor oligonucleotide to the 3’ end of the total RNAs. Next reverse transcription is performed using a primer specific to the adenylated adaptor to initiate the production of cDNA. Then a primer designed near the 3’ end of the transcript of interest is used along with the primer specific to the adenylated adaptor to perform PCR amplification of the poly(A) tail and a short stretch of the 3’ UTR. This step may be modified to amplify specific miRNA sequences. Finally, the PCR products are separated on an acrylamide gel and visualized using either SYBR Gold Nucleic Acid Stain or ethidium bromide.
Product ALL-TAIL™ Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 5205
Quantity 20 Ligations, 30 RT Rxns, 30 PCR Rxns
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122
Email: info@biooscientific.com



Scientific News
The Rise of 3D Cell Culture and in vitro Model Systems for Drug Discovery and Toxicology
An overview of the current technology and the challenges and benefits over 2D cell culture models plus some of the latest advances relating to human health research.
New NIH-EPA Research Centers to Study Environmental Health Disparities
Scientists will partner with community organizations to study these concerns and develop culturally appropriate ways to reduce exposure to harmful environmental conditions.
Structure of Essential Digestive Enzyme Uncovered
Using a powerful combination of techniques from biophysics to mathematics, researchers have revealed new insights into the mechanism of a liver enzyme that is critical for human health.
Air Pollution Linked to Heart Disease
10-year project revealed air pollutants accelerate plaque build-up in arteries to the heart.
Getting a Better Look at How HIV Infects and Takes Over its Host Cells
A new approach, developed by a team of researchers led by The Rockefeller University and The Aaron Diamond AIDS Research Center (ADARC), offers an unprecedented view of how a virus infects and appropriates a host cell, step by step.
Following Tricky Triclosan
Antibacterial product flows through streams, crops.
Vitamin A May Help Improve Pancreatic Cancer Chemotherapy
The addition of high doses of a form of vitamin A could help make chemotherapy more successful in treating pancreatic cancer, according to an early study by Queen Mary University of London (QMUL).
Poverty Marks a Gene, Predicting Depression
New study of high-risk teens reveals a biological pathway for depression.
World’s Largest Coral Gene Database
‘Genetic toolkit’ will help shed light on which species survive climate change.
A Boost for Regenerative Medicine
Growing tissues and organs in the lab for transplantation into patients could become easier after scientists discovered an effective way to produce three-dimensional networks of blood vessels, vital for tissue survival yet a current stumbling block in regenerative medicine.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,100+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!