The Automated Barcode Applicator prints and applies barcodes to microplates, PCR plates and tube racks. It has automated software-driven barcode creation and application. The printed label can be on any of the four sides of the destination item and in any combination of the four side surfaces. The software is designed to allow the user to input a numeric sequence and quantity to be applied, which can be alpha numeric sequences with the inclusion of date and time. Alternatively the entire system can be integrated and run directly via a data input text file.