Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Products>This Product
  Products


1by1 with HTRF®

Product Description
1-by-1 with HTRF interaction assay is based on Homogeneous Time-Resolved Fluorescence, a highly sensitive and robust Time-Resolved FRET technique. This service allows you to test for direct binding in vitro.

Our offer includes :
- Scientific assistance from our protein-interaction experts
- Cloning
- Protein production and purification: both candidate proteins are produced in bacteria as fusions to different tags
- Protein labelling: each protein is labelled either directly or indirectly with anti-tag antibodies coupled to fluorescence donor and acceptor
- Competition test: to control that proteins are well recognized by the antibodies and to evaluate the optimal protein concentrations.
- HTRF interaction test
- Detailed report delivery with protocols, gel pictures, grpahics and comments

Our multi-tag approach and the use of the two donor-acceptor orientations for each protein pair allow for systematically investigating a large panel of combinations, thus increasing the chance of proper protein folding and fluorescence transfer.

Because the assay is homogeneous and requires only low nanomolar concentrations of proteins, multiple interactions can be evaluated simultaneously in a 384-well format.

This makes 1-by-1 with HTRF the perfect output to confirm ULTImate Y2H™ interactions in another system for publication! Our HTRF and Y2H plasmids are fully compatible for direct and efficient subcloning.

For more details, please go to: http://www.hybrigenics-services.com/contents/our-services/validate/1-by-1-with-htrf

HTRF® is a registered trademark of Cisbio International.

Product 1by1 with HTRF®
Company Hybrigenics Services SAS
Price Request a quote
More Information View company product page
Catalog Number Unspecified
Quantity Unspecified
Company Logo

Hybrigenics Services SAS
3-5 Impasse Reille 75014 Paris France

Tel: +33 (0)1 58 10 38 29
Fax: +33 (0)1 58 10 38 48
Email: services@hybrigenics.com



Scientific News
The Rise of 3D Cell Culture and in vitro Model Systems for Drug Discovery and Toxicology
An overview of the current technology and the challenges and benefits over 2D cell culture models plus some of the latest advances relating to human health research.
Grant Supports Project To Develop Simple Test To Screen For Cervical Cancer
UCLA Engineering announces funding from Bill and Melinda Gates Foundation.
Injecting New Life into Old Antibiotics
A new fully synthetic way to make a class of antibiotics called macrolides from simple building blocks is set to open up a new front in the fight against antimicrobial drug resistance.
Insight into Bacterial Resilience and Antibiotic Targets
Variant of CRISPR technology paired with computerized imaging reveals essential gene networks in bacteria.
Advancing Protein Visualization
Cryo-EM methods can determine structures of small proteins bound to potential drug candidates.
Alzheimer’s Protein Serves as Natural Antibiotic
Alzheimer's-associated amyloid plaques may be part of natural process to trap microbes, findings suggest new therapeutic strategies.
Slime Mold Reveals Clues to Immune Cells’ Directional Abilities
Study from UC San Diego identifies a protein involved in the directional ability of a slime mold.
How Do You Kill A Malaria Parasite?
Drexel University scientists have discovered an unusual mechanism for how two new antimalarial drugs operate: They give the parasite’s skin a boost in cholesterol, making it unable to traverse the narrow labyrinths of the human bloodstream. The drugs also seem to trick the parasite into reproducing prematurely.
Illuminating Hidden Gene Regulators
New super-resolution technique visualizes important role of short-lived enzyme clusters.
Supressing Intenstinal Analphylaxis in Peanut Allergy
Study from National Jewish Health shows that blockade of histamine receptors suppresses intestinal anaphylaxis in peanut allergy.
Scroll Up
Scroll Down

Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,100+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!