Tecan has combined its powerful Infinite 200 PRO reader with the patent pending Gas Control Module (GCM), offering a comprehensive solution for a wide range of cell-based applications. The combination of the GCM and the Infinite 200 PRO creates the first multimode reader offering simultaneous, independent control of CO2 and O2 concentrations, providing consistent physiological conditions for predictable culture growth.
The Infinite 200 PRO is a user-friendly, affordable multimode reader available with a choice of Quad4 Monochromators™- or filter-based technologies, offering excellent sensitivity, multiplexing capabilities and high format flexibility, including 6- to 384-well microplates, PCR plates, cuvettes and Tecan’s patented NanoQuant Plate™ for sample volumes as low as 2 µl. With advanced optics and high performance detectors, optimized for the requirements of fluorescence, luminescence and absorbance reading, this easy-to-use modular instrument offers outstanding sensitivity and unparalleled performance in a wide range of detection modes, including fluorescence intensity (FI) top and bottom reading, FRET, TR-FRET, TRF, fluorescence polarization, flash and glow luminescence, dual-color luminescence (including BRET1™ and BRET2 applications), absorbance, and AlphaScreen® and AlphaLISA® technology.
The Infinite 200 PRO’s enhanced FI bottom reading with Optimal Read (OR) function and temperature-controlled linear and orbital shaking offer superior performance for enhanced enzymatic, bacterial and cell-based measurements. Condensation in lidded assay plates is avoided by top heating, and the excellent intra- and inter-well reproducibility provides increased sensitivity for adherent cells. Automated z-focusing with background correction offers automatic optimization of the signal-to-noise ratio, which is ideal for cell-based applications using autofluorescent growth media.
The innovative GCM ensures rigorous environmental control within the detection chamber, with simultaneous, independent control of CO2 and O2 to help maintain stable culture conditions during prolonged experiments, extending the experimental window while ensuring consistent and biologically relevant data. It also allows assays to be performed under anaerobic or physiological conditions, enabling oxygen levels to be reduced to <1 % for applications such as hypoxia-induced factor (HIF) analysis. The unique altitude correction, plus acoustic and visible warnings that alert the user in the event of a problem, guarantee precise and stable regulation of gas concentration inside the reader chamber.
For increased walkaway times and secure overnight operation, the Infinite 200 PRO reader with GCM can be remotely monitored by Tecan’s Common Notification System (CNS) through any networked computer or mobile device. This true walkaway operation eliminates the gaps in data that potentially occur during overnight experiments or studies carried out over an extended period of time, minimizing the number of repeat assays for improved productivity and even more cost-effective operation.
*For research use only