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Friday, October 31, 2014
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World of Technology & Science 2014...
PCR Setup Solution for OEM liquid handli...Bio-Rad Droplet Digital™ PCR and Organ T...Digital PCR in the Clinical Virology Lab...

Featured Products
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RainDrop Digital PCR System

Webcasts
Finding Needles in a Haystack with Q-PCR – Rare Mutations, Single Cell Genotyping and DNA Methylation Analysis
Jorg Tost, Centre National de Genotypage CEA, speaking at Advances in qPCR and dPCR 2014
Evidence Based Guidelines for the Pre-analytical Phase of RNA Testing in Blood Samples
Francesca Malentacchi, University of Florence, Speaking at Advances in qPCR and dPCR 2014

Market Reports
Gene Expression Analysis Market by Technology, Consumables, Services & Applications - Global Forecast to 2018
MarketsandMarkets

Over the years, the gene expression analysis market, comprising of instruments, consumables, and services, has witnessed various technological advancements. These advancements have led to a growth in the number of gene expression applications.
Food Diagnostics Systems Market, Consumables Market & By Geography - Global Trends & Forecast To 2018
MarketsandMarkets

The food diagnostics market size is projected to increase with rising concerns over food quality and safety, and the implementation of stringent regulations on food safety and foodborne illness prevention.
Cell Analysis Market by Instruments, Consumables & Application - Global Forecast to 2018
MarketsandMarkets

The global cell analysis market is a technology-driven market and is marked by the ?Threat of Obsolescence,? wherein technologies as well as their adoption by end users changes very rapidly. Hospitals, academic and government institutes, as well as pharmaceutical and biotechnology companies are increasing their focus on using cell analysis as a powerful research tool in research, drug discovery, and diagnosis.
Life Science Software 2013: Markets and Opportunities
Biopharm Reports

This reports into in the life science field examines the use of specialist software and databases relating to the conduct of PCR, DNA sequencing, DNA microarray, flow cytometry, mass spectrometry and disease biomarker studies. The studies were carried out to assist software developers and vendors to identify market opportunities in these fields.
Life Science Consumables 2013: Markets and Opportunities
Biopharm Reports

Techniques such as PCR, DNA microarray, DNA sequencing, mass spectrometry and flow cytometry have grown significantly in the last decade and by 2015 markets for these five areas are expected to exceed $50 billion, globally. In these and related fields, consumables are generally the second or third largest area in cost terms, after instrumentation.
qPCR Assay Trends 2013
HTStec

HTStec's qPCR Assay Trends 2013 report was published on 15 April 2013. This 63 page market report summarizes the results of HTStec's 3rd industry-wide global web-based benchmarking survey on real-time quantitative PCR (qPCR) assays and instruments carried out in March 2013.
Benchtop Automation Trends 2013
HTStec

This market report summarizes the results of HTStec's industry-wide global web-based benchmarking survey on small-scale or benchtop automation carried out in January 2013.
Lab Instrument Rental Trends 2012
HTStec

This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on lab instrument rental carried out in September 2012.
Live Cell Kinetic Imaging Trends
HTStec

This market report summarizes the results of HTStec’s 2nd global web-based benchmarking survey on live cell kinetic imaging assays and instruments carried out in May 2012.
High Throughput qPCR Assay Trends
HTStec

This market report summarizes the results of HTStec’s global web-based benchmarking survey on high throughput real-time quantitative PCR (HT qPCR) assays carried out in January 2012.
Scientific News
CDC Develops a New, Faster Lab Test for Enterovirus D68
Confirmed cases will appear to rise as agency accelerates specimen testing.
NanoString, Brigham and Women’s Hospital Collaborate
Research collaboration to accelerate translation of genomic discoveries into clinical diagnostics in oncology.
Unlocking The Non-Coding Half of Human Genome
Texas A&M biologists unlock non-coding half of human genome with novel DNA sequencing technique.
Improving Trout Resistance to a Deadly Disease
A highly sensitive real-time PCR test has been developed to measure F. psychrophilum in fish tissue.
Blood Test To Diagnose Depression
Test identifies nine blood markers tied to depression; predicts who will benefit from therapy.
Redefining High Risk Patients With Stage II Colon Cancer
microRNA-21 identified as an independent prognostic biomarker.
More People Testing Positive for Chikungunya Virus in the U.S.
Clinical testing volume and positivity rate both grew after the first case of the infectious disease spread to Western Hemisphere this year.
Novel rhPCR Unravels Gene Expression
Method enables identification and analysis of highly similar alternatively spliced transcripts.
Detecting and Identifying Candida Species in Blood Samples of Critically Ill Paediatric Patients
The study aimed to develop a multiplex nested PCR method to detect and identify seven Candida species in peripheral blood samples of critically ill paediatric patients.
IDT Shares Comprehensive qPCR Resources
Special qPCR compendium issue of DECODED now freely available.
Business News
QIAGEN Announces Broad Agreement with Astellas Pharma
First two programs aim to create tests to aid in cancer treatment with Astellas compounds under development.
Clontech Licenses CRISPR/CAS9 Technology From Broad Institute
Clontech Laboratories, Inc. have entered into a non-exclusive license agreement with the Broad Institute to access intellectual property related to the CRISPR/Cas9 gene editing system.
HudsonAlpha Named Latest RainDance Center of Excellence
Premier biotech research center adopts complete RainDance product portfolio to enhance next-generation sequencing and digital PCR services offering.
Transgenomic Announces $2.375 Million Private Placement Financing
Net proceeds from this offering will support Company’s ICE COLD-PCR™ technology.
TGen and NAU Patent for New Pandemic Flu Test is Approved
H1N1 assay benefits patients by helping doctors determine if infections are resistant to available flu treatments.
Quidel Receives FDA Clearance for its Lyra™ Molecular PCR Assay
A real-time PCR test for the detection of human parainfluenza viruses (HPIV), Types 1, 2 and 3.
RainDance Technologies Appoints John A. Luckey VP of Product Development
Biotechnology professional brings proven experience designing, developing and delivering products for life-science applications.
Alere Awarded U.S. Government Contract to Advance Development of POC Molecular Diagnostic
The U.S. Department of Health and Human Services will fund up to $12.9 million for the development of Alere’s next-generation POC molecular influenza test.
PositiveID Corporation and ENSCO Awarded Contract for SenseNet Program
PositiveID will offer its Firefly Dx system under the SenseNet program for faster, less expensive bio-threat detection.
Biogazelle Attracts New Investors
UGent spin-off Biogazelle attracts new investors QBIC Arkiv Fund, Foundation Majoie and SOFI to finance growth.

Events
Lab-on-a-Chip Asia
20 Nov 2014
Lab-on-a-Chip Asia ...
Pittcon 2015
08 Mar 2015
Pittcon 2015 ...
7th International qPCR & NGS E
23 Mar 2015
7th International qPCR & NGS E ...
Advances in qPCR & dPCR
21 May 2015
Advances in qPCR & dPCR ...

ePosters
Digital PCR to Determine the Number of Transcripts from Single Neurons after Patch-clamp Recording
1AVIDIN Ltd., Szeged, Hungary www.avidinbiotech.com; 2Laboratory for Functional Genomics, Biological Research Center, HAS; 3Research Group for Cortical Microcircuits, HAS, Department of Physiology, An

Whole-cell patch-clamp recording enables detecting electrophysiological signals from neurons, and RNA can be harvested into the patch pipette from the cells.We have optimized a dPCR protocol for determining exact transcript numbers in single neurons after patch-clamp recording by using dPCR based on high-density nanocapillary PCR.
On-chip quantification of miRNA using digital droplet PCR
1. Life Science Technologies, Imec, Kapeldreef 75, Heverlee, Belgium 2. Advanced Technology Research Laboratory, Panasonic Corporation, Kyoto Japan

miRNAs have a great potential in diagnostics. Hence, automated profiling of miRNAs are of great interest. In-house technology show that it is possible to implement a multiplexing assay for miRNAs on a microfluidic chip using digital droplet PCR.
A multianalyte algorithm PCR-based blood test outperforms single analyte ELISA-based blood tests for neuroendocrine tumor detection
Wren Laboratories

In a prospective study, in age-/sex- and ethnicity-matched patients and controls (n=82), a 51 panel multigene blood transcript test (NETest) was identified to be significantly more sensitive and accurate (>93%) than any single analyte assay (Chromogranin A, Pancreastatin or Neurokinin A) for neuroendocrine tumor detection.
Stealth-Adapted Viruses and Viteria: Insights into Virus Construction, Replication and Potential Therapies
Institute of Progressive Medicine

There is an increasing incidence of diseases with accompanying signs and symptoms of brain damage. These include neurological and psychiatric illnesses, childhood behavioral disorders, and such common conditions as chronic fatigue, Gulf War Syndrome, so-called “chronic Lyme disease”, and many cancers. Altogether, these diseases have an enormous social impact.
High-Throughput Analysis of DNA Samples using the D1K ScreenTape Assay and the Agilent 2200 TapeStation System
Agilent Technologies

Recent advances in genomics demands to look at a wealth of genetic information in a short period of time. DNA analysis using slab gel electrophoresis and capillary electrophoresis are widely being used as a QC step in next generation sequencing and microarray studies. However, often these techniques lack the speed and involve more manual steps to perform the assay.
Hot Start dNTPs – Pushing the Limits of PCR
Agilent Technologies

Hot Start dNTPs are a distinct approach that employs modified nucleoside triphosphates with a thermolabile protecting group. This modification blocks low temperature primer extension and is released at higher temperatures to allow for more specific DNA polymerase incorporation.
RNA Quality Control using the Agilent 2200 TapeStation System –Assessment of the RINe Quality Metric
Agilent Technologies

Here, we present a comparative study between the RINe quality score obtained from R6K ScreenTape and High Sensitivity R6K ScreenTape compared to the RIN quality metric obtained from the 2100 Bioanalyzer system.
Simultaneous RT-qPCR Measurement of 1718 Long Non-Coding RNAs
biogazelle

Massively parallel RNA-sequencing revealed that the human genome is pervasively transcribed, resulting in the production of thousands of non-coding RNA transcripts.
Advanced Copy Number Variant Analysis with qbasePLUS 2
biogazelle

Copy number changes under the form of deletions and duplications are known to be involved in numerous human genetic disorders. Moreover, each individual’s genome embodies several copy number polymorphisms of various sizes which are thought to contribute to normal phenotypic variation and susceptibility to multifunctional disease.
ChIP-qPCR and qbasePLUS Jointly Identify a MYCN Activated miRNA Cluster in Cancer
biogazelle

This study applies ChIP-qPCR tp assess binding of transcription factor MYCN to miRNA cluster 17-92, to positive control target MDM2, and to a negative control target region.
Application Notes
Double stranded DNA quantitation using Promega QuantiFluor® Dyes
Tecan Group Ltd.

Achieving selective and sensitive quantitation of double stranded DNA (dsDNA) using spectrophotometric methods can be problematic. Recognizing these difficulties, researchers have incorporated fluorescence-based quantitation techniques in their workflows to obtain the required sensitivity and target selectivity.
Rapid, High Throughput Extraction and Purification of Genomic DNA from Whole Blood Using the Akonni TruTip® Extraction System on the Hamilton Microlab® STAR Liquid Handling System
Hamilton

This application note demonstrates the high precision of the automated extraction system in processing genomic DNA from low volume whole blood samples and its advantage over competitors.
Automated GS FLX Titanium emPCR Enrichment using the REM e System on a Hamilton Microlab® STARlet Liquid Handler
Hamilton

The Genome Sequencer FLX System is a versatile sequencing platform suitable for a wide range of research applications, including de novo sequencing and assembly of genomic DNA, transcriptome sequencing, small RNA analysis, and amplicon sequencing.
A MIQE Case Study — Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data
Bio-Rad Laboratories

In this study, the minichromosome maintenance (MCM) protein, MCM7, was selected as a model target gene to investigate the importance of appropriate reference gene selection and RNA sample quality as described by the MIQE guidelines.
Sequence-Specific DNA Assay
Molecular Devices

The Threshold® system from Molecular Devices, together with the Immuno Ligand Assay (ILA) kit can be used to measure specific DNA sequences. Biotinylated or fluorescienated oligonucleotide probes, specific to the target sequence, anneal to the digested target DNA creating a probe-target hybrid which can then be detected and quantified against a standard curve, with pleasing results.
High Yield and Quality with Thermo Scientific Microtiter Deep Well 96 plates in KingFisher Flex Process
Thermo Fisher Scientific

The Thermo Scientific KingFisher Flex magnetic particle processor is specifically designed to automate the time-consuming sample preparation of proteins, nucleic acids and cells in 96-well plate formats.
Isolation and Purification of Genomic DNA and Viral RNA from Clinical Samples with Thermo Scientific KingFisher Flex
Thermo Fisher Scientific

The purification of DNA and RNA from clinical samples requires rapid and reliable processing of large quantities of samples. Automated purification of nucleic acids, proteins and cells with Thermo Scientific KingFisher Flex is a reproducible and high quality method, which produces excellent material for further analyses.
Efficient Delivery of Thermo Scientific Dharmacon SMARTpool siRNA Reagents in Difficult-to-Transfect Cell Lines Using Nucleofector® 96-well Shuttle® System
Lonza

siRNA-mediated gene knockdown is a powerful tool that has been used to identify gene function and elucidate biological pathways. Successful siRNA experiments involving knockdown of individual genes or collections of gene targets require efficient delivery of highly functional and specific siRNA molecules into appropriate cells.
ScreenTape® Enables Fast Quality Control of miRNA qPCR Reactions
AnaSpec

The ScreenTape® System, developed by Lab901, employs a unique microscale polymer tape that contains all the reagents required for DNA electrophoresis. It brings major advantages of convenience, ease of use and safety to molecular biologists as absolutely no handling of gels and buffers is required.
Advanced Software for Real-Time PCR Data Analysis by Integromics
Integromics

Got data? Get answers! Real-Time StatMiner™ is workflow oriented: a step-by-step guide to ensure reliable and accurate results using powerful statistics and interactive visualizations. Average time of analysis for all skill level users- just 10 minutes! Steps of the analysis consist of importing raw Ct data, performing quality control and outlier detection, examining correlation between biological replicates, selecting the optimal combination of endogenous controls for normalization based on sta

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