Evaluation of Candidate Reference Genes for Gene Expression Normalization
Study performed in Brassica juncea using real time quantitative RT-PCR.

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Reliable and Rapid Characterization of Functional FCN2 Gene Variants Reveals Diverse Geographical Patterns
In this study, scientists genotyped and characterized four FCN2 functional SNP variants in West Africans, South Americans, South East Asians from Vietnam and Caucasians from Germany using an available rapid, reliable and cost effective FRET-RTPCR methodology.

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The Potential of TaqMan Array Cards for Detection of Multiple Biological Agents by Real-Time PCR
The TaqMan Array Card architecture, normally used for gene expression studies, was evaluated for its potential to detect multiple bacterial agents by real-time PCR.

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Selection of Suitable Reference Genes for RT-qPCR Analyses in Cyanobacteria
In this work we have validated candidate reference genes for accurate normalization of RT-qPCR data in cyanobacteria. Several genes were selected and their transcription analyzed in three morphological distinct organisms.

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Dr. Derek Wildman Completes Genome Sequence of Great Ape Living With Humans
A Wayne State University School of Medicine researcher is one step closer to understanding the genetic basis that enable bonobos to learn language, play music and use rudimentary tools.

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DNA Sequencing Lays Foundation for Personalized Cancer Treatment
Scientists at Washington University School of Medicine in St. Louis are using powerful DNA sequencing technology to identify mutations at the root of a patient's tumor and to map the genetic evolution of disease and monitor response to treatment.

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Complete Genomics Now Accepts DNA Obtained From Saliva for Whole Human Genome Sequencing
Further expansion of service offerings and collaboration with DNA Genotek allows more researchers with saliva samples access to highly accurate sequencing data.

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ACMG Releases Policy Statement on Genomic Sequencing
ACMG Policy Statement titled "Points to Consider in the Clinical Application of Genomic Sequencing" outlines recommended principles as genome-scale DNA sequencing begins to be applied in the care of patients.

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Comparison of Real-Time Quantitative PCR and Culture for the Diagnosis of Emerging Rickettsioses
Isolation of Rickettsia species from skin biopsies may be replaced by PCR. In this stidy, scientists evaluated culture sensitivity compared to PCR based on sampling delay and previous antibiotic treatment.

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Stanford Scientist Omics Profile used to Discover, Track his Diabetes Onset
Researchers also spied on Dr Snyder's immune system and watched it battle viral infections.

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Patent Protection for Large-Scale DNA Production Technology
Vandalia Research, Inc. announced that the US Patent and Trademark Office granted U.S. Patent No. 8,163,489, covering Vandalia's process for large-scale DNA production using polymerase chain reaction (PCR).

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GenDx Obtains World Wide Distribution Rights of QIAGEN® LongRange PCR Kit
Performance of GenDx SBTexcellerator® reagents for tissue typing diagnostics in combination with LongRange PCR kit offers best HLA-typing results.

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Phthisis Diagnostics Welcomes New Sales Director and Controller
Appointment of Betty Polk and Robert Owen to Company’s management team.

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Eureka Genomics Launches a Novel Custom Genotyping Service
This platform addresses the gap between traditional array technologies and multiplex PCR.

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Beckman Coulter, Inc. Obtains CLIA Certificate, Licensure for Clinical Sequencing
Certification paves the way for genetic testing of patient samples by Beckman Coulter Genomics.

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ZyGEM Teams with TATAA Biocenter
Quantitative PCR expert adds ZyGEM DNA and RNA extraction kits to its select product offerings.

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Fluidigm China Opens Its Doors
Company expects to rapidly build strong presence in country and improve services to Chinese customer base.

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Analytik Jena Increases Stake in its Subsidiary AJ Roboscreen to 100%
The Jena-based manufacturer of analytical instrumentation technology, life science instruments and optoelectronics has thus integrated another subsidiary further into the Group.

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QIAGEN Receives FDA Clearances for Rotor-Gene Q MDx Instrument and Compatible Influenza A/B Assay
Both products receive 510(k) clearance for in vitro diagnostic use in the U.S..

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Lab21 Partners with Top 10 IVD Player
Agreement to develop, manufacture and distribute new Aspergillus molecular assay.

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Noval, Fully Auromated Method Allows Efficient Analysis of qPCR Data for Qualitative Calling Based on Comparative Cq
Azure PCR Limited

Assessment to ascertain if a method for analysis of qPCR data dependent on manual intervention can be replaced by automated analysis using the AzurePCRTM method.

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Volume-Related Inhibitors Standardization for Reverse Transcription Quantitative Polymerase Chain Reaction Experiments
IRBA

This poster addresses the reliability of qPCR data and its dependence on technical variations. The proposal is that constant volume of RNA extract in order to improve reliability.

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Defining off-target cleavage in a pair of Zinc Finger Nucleases
University of Utah

This study looks at off-target cleavage of Zinc Finger Nucleases (ZNFs) in Drosophila in an attempt to analyze potential cleavage spots, with a view to designing more efficient ZFNs.

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Gene Expression Profiling: qPCR Toolkit for Quality Control
TATAA Biocenter

TATAA Biocenter explain how they developedand optimized high-throughput gene expression qPCR with ValidPrime quality control, compensating for inter-run variations.

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Rapid PCR for Integration in Sample-to-answer Analysis Platforms
Institut fuer Mikrotechnik Mainz

This poster describes how molecular testing at the point-of-care can increase time to results and yield rather specific information, concentrating on PCR on a chip layout which proves to be fast and robust.

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Hot Start Amplification using OligoBeads via Gradual Release of Bound Primers
CTGen

OligoBeads provide a mean to store normalized primers used in performing enzymatic reactions including PCR. Primer bound beads eliminate the potential for pipeting errors and reduce contamination thus yielding lower repeat rates and less reagent wastage. The primers bound to the OligoBeads can be stored over a period of a few months without degradation in a nuclease free environment.

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Target length effect on sensitivity and specificity of oligonucleotide microarrays: Advantages of a modified PCR based labelling method over the dendrimer technology.
1 College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK 2 Systems Biology Ireland and the Conway Institute, University College Dublin, Belfield, Dublin 4, Irel

Several methods have been developed for target labelling to enable DNA microarray quantification without taking careful consideration for target length effect. This report highlights the importance of choosing the optimum target length that would ensure specificity without compromising sensitivity of the assay. It also shows the advantages of using the modified PCR method over other methods in generating labelled amplicons of the desired lengths to maximize hybridization efficiency.

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On chip micro-extraction and real-time PCR with integrated SPAD optical fluorescence detection for nucleic acid analysis
FBK - CMM

A PDMS lab-on-a-chip for one step DNA isolation and real time-polymerase chain reaction (RT-PCR) has been designed, fabricated, and characterized for point-of-care clinical diagnostics. In addition, a module for on-chip optical detection based on SPAD - Single-Photon Avalanche Diode - detector has also been developed and used to monitor the presence of specific DNA polymorphisms possibly related to genetic diseases.

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Chemical Variant of 7-deaza-dGTP for Improved CG-rich PCR Amplification
TriLink BioTechnologies, Inc.

PCR amplification of nucleic acids is a fundamental technique used in many molecular biology laboratories. Despite its wide use, certain GC-rich regions of DNA, such as mycobacterial disease targets, still remain a challenge for amplification. Sequences high in GC content are associated with the formation of secondary structure, which prevents adequate strand separation and DNA polymerase amplification. As a consequence, mispriming is prominent, complicating specific product formation.

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Periodontal Pockets Microbiota qPCR Analysis at Different Stages of Periodontitis
DNA-Technology

Six major periodontal pathogens were quantified (with standardization to human gDNA) in periodontal pockets of periodontitis patients and healthy subjects by original qPCR assay. Relative amount of pathogens to total bacterial mass increased for P.gingivalis (100-fold), P.intermedia (10-fold) and T.forsythensis (10-fold).

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A MIQE Case Study — Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data
Bio-Rad Laboratories

In this study, the minichromosome maintenance (MCM) protein, MCM7, was selected as a model target gene to investigate the importance of appropriate reference gene selection and RNA sample quality as described by the MIQE guidelines.

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Sequence-Specific DNA Assay
Molecular Devices

The Threshold® system from Molecular Devices, together with the Immuno Ligand Assay (ILA) kit can be used to measure specific DNA sequences. Biotinylated or fluorescienated oligonucleotide probes, specific to the target sequence, anneal to the digested target DNA creating a probe-target hybrid which can then be detected and quantified against a standard curve, with pleasing results.

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High Yield and Quality with Thermo Scientific Microtiter Deep Well 96 plates in KingFisher Flex Process
Thermo Fisher Scientific

The Thermo Scientific KingFisher Flex magnetic particle processor is specifically designed to automate the time-consuming sample preparation of proteins, nucleic acids and cells in 96-well plate formats.

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Isolation and Purification of Genomic DNA and Viral RNA from Clinical Samples with Thermo Scientific KingFisher Flex
Thermo Fisher Scientific

The purification of DNA and RNA from clinical samples requires rapid and reliable processing of large quantities of samples. Automated purification of nucleic acids, proteins and cells with Thermo Scientific KingFisher Flex is a reproducible and high quality method, which produces excellent material for further analyses.

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Efficient Delivery of Thermo Scientific Dharmacon SMARTpool siRNA Reagents in Difficult-to-Transfect Cell Lines Using Nucleofector® 96-well Shuttle® System
Lonza

siRNA-mediated gene knockdown is a powerful tool that has been used to identify gene function and elucidate biological pathways. Successful siRNA experiments involving knockdown of individual genes or collections of gene targets require efficient delivery of highly functional and specific siRNA molecules into appropriate cells.

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ScreenTape® Enables Fast Quality Control of miRNA qPCR Reactions
AnaSpec

The ScreenTape® System, developed by Lab901, employs a unique microscale polymer tape that contains all the reagents required for DNA electrophoresis. It brings major advantages of convenience, ease of use and safety to molecular biologists as absolutely no handling of gels and buffers is required.

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Advanced Software for Real-Time PCR Data Analysis by Integromics
Integromics

Got data? Get answers! Real-Time StatMiner™ is workflow oriented: a step-by-step guide to ensure reliable and accurate results using powerful statistics and interactive visualizations. Average time of analysis for all skill level users- just 10 minutes! Steps of the analysis consist of importing raw Ct data, performing quality control and outlier detection, examining correlation between biological replicates, selecting the optimal combination of endogenous controls for normalization based on sta

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Identify optimal reference genes for realtime-qPCR with GenEx MultiD Analyses AB
MultiD Analyses AB

Tailor-made for qPCR. Powerful. Easy to use. GenEx is a popular software for qPCR data processing and analysis. Built in a modular fashion GenEx provides a multitude of functionalities for the qPCR community, ranging from basic data editing and management, such as data pre-processing and group assignments, to advanced cutting-edge data analysis, such as PCA and ANN.

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BEBO-for qPCR and HRM
TATAA Biocenter

BEBO is an unsymmetric cyanine dye developed by TATAA Biocenter for use in qPCR applications. The dye has absorbance and emission wavelengths that can be detected on the FAM channel on most common real-time PCR platforms, and shows a strong fluorescence increase when bound to dsDNA. BEBO can be used as an unspecific dye for real-time PCR applications or other applications where staining of dsDNA is wanted.

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BOXTO-for qPCR applications
TATAA Biocenter

BOXTO is a derivative of BEBO, an unsymmetric cyanine dye developed by TATAA Biocenter for use in qPCR applications. The dye has absorbance and emission wavelengths that can be detected on the JOE channel on most common real-time PCR platforms, and shows a very strong fluorescence increase when bound to dsDNA.

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