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Maxwell® RSC: Enhance your efficiency with the next generation in automated nucleic acid purification

Webcasts
Evolution and Selection of Soybean Nematode Resistance Locus
Tong Geon Lee, University of Illinois at Urbana Champaign, speaking at Advances in Plant Genomics 2014
Characterizing the Early Drug Development Outsourcing Landscape
Stella Stergiopoulos, Tufts Center for the Study of Drug Design, speaking at Drug Discovery and Development 2014

Market Reports
Gene Expression Analysis Market by Technology, Consumables, Services & Applications - Global Forecast to 2018
MarketsandMarkets

Over the years, the gene expression analysis market, comprising of instruments, consumables, and services, has witnessed various technological advancements. These advancements have led to a growth in the number of gene expression applications.
Food Diagnostics Systems Market, Consumables Market & By Geography - Global Trends & Forecast To 2018
MarketsandMarkets

The food diagnostics market size is projected to increase with rising concerns over food quality and safety, and the implementation of stringent regulations on food safety and foodborne illness prevention.
Cell Analysis Market by Instruments, Consumables & Application - Global Forecast to 2018
MarketsandMarkets

The global cell analysis market is a technology-driven market and is marked by the ?Threat of Obsolescence,? wherein technologies as well as their adoption by end users changes very rapidly. Hospitals, academic and government institutes, as well as pharmaceutical and biotechnology companies are increasing their focus on using cell analysis as a powerful research tool in research, drug discovery, and diagnosis.
Life Science Software 2013: Markets and Opportunities
Biopharm Reports

This reports into in the life science field examines the use of specialist software and databases relating to the conduct of PCR, DNA sequencing, DNA microarray, flow cytometry, mass spectrometry and disease biomarker studies. The studies were carried out to assist software developers and vendors to identify market opportunities in these fields.
Life Science Consumables 2013: Markets and Opportunities
Biopharm Reports

Techniques such as PCR, DNA microarray, DNA sequencing, mass spectrometry and flow cytometry have grown significantly in the last decade and by 2015 markets for these five areas are expected to exceed $50 billion, globally. In these and related fields, consumables are generally the second or third largest area in cost terms, after instrumentation.
qPCR Assay Trends 2013
HTStec

HTStec's qPCR Assay Trends 2013 report was published on 15 April 2013. This 63 page market report summarizes the results of HTStec's 3rd industry-wide global web-based benchmarking survey on real-time quantitative PCR (qPCR) assays and instruments carried out in March 2013.
Benchtop Automation Trends 2013
HTStec

This market report summarizes the results of HTStec's industry-wide global web-based benchmarking survey on small-scale or benchtop automation carried out in January 2013.
Lab Instrument Rental Trends 2012
HTStec

This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on lab instrument rental carried out in September 2012.
Live Cell Kinetic Imaging Trends
HTStec

This market report summarizes the results of HTStec’s 2nd global web-based benchmarking survey on live cell kinetic imaging assays and instruments carried out in May 2012.
High Throughput qPCR Assay Trends
HTStec

This market report summarizes the results of HTStec’s global web-based benchmarking survey on high throughput real-time quantitative PCR (HT qPCR) assays carried out in January 2012.
Scientific News
Single-cell Analysis Hits its Stride
Advances in technology and computational analysis enable scale and affordability, paving the way for translational studies.
New Genomic Research Amends Earlier Triple Negative Breast Cancer Finding
Previously reported molecular finding unable to be validated.
LifeCodexx Reports Successful Proof-of-Principle of Innovative PCR-based NIPT Assay
Breakthrough potential as affordable NIPT solution on global basis.
Blood Test Trumps Accuracy of Standard Screening in Detecting Down Syndrome in Early Pregnancy
A blood test undertaken between 10 to 14 weeks of pregnancy may be more effective in diagnosing Down syndrome and two other less common chromosomal abnormalities than standard non-invasive screening techniques.
Highly Sensitive Detection Of Malaria Parasites
New assays can detect malaria parasites in human blood at very low levels and might be helpful in the campaign to eradicate malaria, reports a study published in PLOS Medicine.
Selection of Reference Genes for Quantitative RT-PCR Analysis
The expression stability of 12 commonly used reference genes was analysed.
‘Liquid Biopsies’ Could Help Spot Genetic Faults in Lung Cancer
Study analyze blood samples from 97 patients who took part in the EURTAC clinical trial.
Accurate Determination of Copy Number States for Multiallelic Copy Number Variations
Researchers use next-generation sequencing (NGS) and Bio-Rad’s Droplet Digital PCR (ddPCR™) technology to solve the technical challenge.
More DNA & Extra Copies of Disease Gene in Alzheimer’s Brain Cells
Scientists at The Scripps Research Institute (TSRI) have found diverse genomic changes in single neurons from the brains of Alzheimer’s patients, pointing to an unexpected factor that may underpin the most common form of the disease.
Single-Cell, 42-plexed Protein Analysis Achieved with a New Microchip Technology
A novel microdevice capable of detecting 42 unique immune effector proteins has been developed.
Business News
Pacific Biosciences and RainDance Technologies Partner
Partnership combines Pacific Biosciences’ Single Molecule, Real-Time (SMRT®) Sequencing and informatics capabilities with RainDance’s proprietary digital droplet and single molecule barcoding technology.
WHO Approves Ebola Assay
Xpert Ebola is now eligible for procurement to Ebola-affected countries
cytena Received EUR 1.1 Million of Seed Capital
Company has developed a device called cy-Clone to separate single cells.
WaferGen & Genentech Collaborate
WaferGen’s SmartChipTM platform has potential to accelerate pharmaceutical drug discovery and development.
IDT Opens Molecular Biology R&D Facility in Redwood City
Dr Caifu Chen to lead new product development at California site.
Transgenomic to Incorporate Horizon's Genomic Reference Standards
Horizon to provide human genomic reference standards to assure quality and performance of Transgenomic’s Multiplexed ICE COLD-PCR kits.
Presentations at the AACR Annual Meeting to Highlight Advances in Cancer Research Made Possible by Bio-Rad’s Droplet Digital™ PCR Technology
Tailoring Treatments and Tracking Mutations with Liquid Biopsies possible with ddPCR™ technology
RainDance Technologies Extends Global Commercial Reach
BioTech-Europe, Diatech, Labgene Scientific, and Neo-Science embrace company’s award-winning droplet technology platform to meet growing demand for liquid biopsy research applications.
LC Sciences Seeks Beta Testers of Targeted Sequencing Method Ahead of Q4 Launch
Company will collaborate with beta testers to generate test data sets.
LGC Acquires Biosearch Technologies
Company extends its expertise in the qPCR market.

Events
Genomics & Proteomics Research
28 May 2015
Genomics & Proteomics Research ...
63rd ASMS Conference on Mass S
31 May 2015
63rd ASMS Conference on Mass S ...
qPCR and Digital PCR Congress
25 Jun 2015
qPCR and Digital PCR Congress ...
Food Processing and Analysis
20 Aug 2015
Food Processing and Analysis ...

ePosters
Single Cell Whole Genome Amplification
GE Global Research

The analysis of genomes at the single cell level offers unprecedented biological insights in diverse fields such as cancer research, immunology & microbiology. This poster discusses the a technology for amplification of genomic DNA form a single cell, that provides utmost sensitivity, accuracy & robustness. GE Healthcare Life Sciences had previously developed method of multiple strand displacement amplification (MDA) by Phi29 DNA polymerase
System-Specific Periodicity in qPCR Data and its Impact on Quantitation
1Dept. of Chemistry, Vanderbilt Univ., Nashville, TN USA 37235; 2 BTU Cottbus-Senftenberg, Senftenberg, Germany; 3 Dept. of Andrology, Univ. Hospital Hamburg-Eppendorf, Germany

In some commercial qPCR instruments, the fluorescence signal shows reaction-to-reaction periodicity in all cycles, and this translates into periodicity in threshold-based Cq markers.
A Microwave/Microfluidics Integrated Label-Free Detection and Content Sensing System for Point-of-Care Applications
University of Waterloo

This poster presents a droplet-based microfluidic device integrated with a microwave wave sensor, which is also a heater, for simultaneous sensing and heating of individual nanoliter-sized droplets.
Rapid Detection of Somatic Mutations in Cancer Genes
DiaCarta, Inc.

Rapid higly sensitive detection of tumor gene mutations in DNA derived from FFPE or plasma samples can be achieved with QClamp PCR technology.
Accurate Normalization of Grain Samples Using Pressure-Based Volume Measurement Technology
Artel and STRATEC

A common challenge experienced by sample screening facilities is identifying individual sample quantity prior to DNA extraction. Accurately identifying sample quantity allows the user to pipette exact amounts of extraction buffer into each well.
DHPLC Technology as a High-throughput Detection of Mutations in a Durum Wheat TILLING Population
1Department of Soil, Plant and Food Sciences, sect. Genetics and Plant Breeding, University of Bari, Via Amendola 165/A, 70126 Bari (Italy) 2 Transgenomics Omaha, NE, USA

This study is a beautiful example of DHPLC technology application and shows an alternative tool to current strategies of SNP detection based on genotyping array.
IDENTIFICATION AND DIFERENTIATION OF Verticillium SPECIES WITH PCR MARKERS AND SEQUENCING OF ITS REGION


The genus Verticillium is a group of ascomycete fungi, including plant-pathogenic species capable of affecting the vasculature of many agricultural crops, and therefore causes major economic losses worldwide. In 2011, a new taxonomic classification of the genus was proposed, which is now referred to as Verticillium sensu stricto, comprising ten species: V. dahliae V. albo-atru, V. alfalfae, V. longisporum, V. nonalfalfae, V. tricorpus, V. zaregamsianum, V. nubilum, V. isaacii and V. klebahnii. <
Expression Profiling of Circulating Tumor Cells: a Prognostic and Predictive Biomarker in Cancer.
Bauer4.1TATAA Biocenter, Göteborg, Sweden (www.tataa.com); 2Inst. of Biotechnology, CAS, Czech Republic; 3AdnaGen GmbH, Ostpassage7, D-30853 Langenhagen, Germany (www.adnagen.com); 4Dep of Gynecoland

We show non-responders to treatment among breast cancer patients can be identified by expression profiling of circulating tumor cells
Detection in Environmental Samples of Enteroagregative Escherichia coli, stx-producing Escherichia coli and stx-converting Bacteriophage by RT- PCR: Preliminary Data
Istituto Zooprofilattico Sperimentale della Sardegna- Italy

The aim of this work was to collect epidemiological information concerning the presence of Enteroaggregative Escherichia coli, stx-producing Escherichia coli and stx2a-converting bacteriophage, in ruminants faeces.
Effect of exposure to stress conditions on propidium monoazide (PMA) - qPCR based enumeration of Campylobacter in broiler carcass rinses
1 Laboratory of Food Microbiology and Food Preservation, Dept Food Safety & Food Quality Faculty of Bioscience Engineering, Gent, Belgium 2 Institute of Public Health, Scientific service Food-borne pa

Evaluation and comparison of a developed PMA-qPCR Campylobacter enumeration method with the reference method, after exposure of the broiler rinse artificially contaminated with the bacteria to stress conditions.
Application Notes
Directional qRNA-Seq: Combining the Power of Stranded RNA-Seq with the Quantitative Precision of Molecular Labels
Bioo Scientific

Bioo Scientific introduces a novel product for advanced RNA-Seq library preparation that features strand-specific RNA sequencing and correction of PCR amplification bias by molecular indexing: the NEXTflex™ Rapid Directional qRNA-Seq™ Kit.
Double stranded DNA quantitation using Promega QuantiFluor® Dyes
Tecan Group Ltd.

Achieving selective and sensitive quantitation of double stranded DNA (dsDNA) using spectrophotometric methods can be problematic. Recognizing these difficulties, researchers have incorporated fluorescence-based quantitation techniques in their workflows to obtain the required sensitivity and target selectivity.
Rapid, High Throughput Extraction and Purification of Genomic DNA from Whole Blood Using the Akonni TruTip® Extraction System on the Hamilton Microlab® STAR Liquid Handling System
Hamilton

This application note demonstrates the high precision of the automated extraction system in processing genomic DNA from low volume whole blood samples and its advantage over competitors.
Automated GS FLX Titanium emPCR Enrichment using the REM e System on a Hamilton Microlab® STARlet Liquid Handler
Hamilton

The Genome Sequencer FLX System is a versatile sequencing platform suitable for a wide range of research applications, including de novo sequencing and assembly of genomic DNA, transcriptome sequencing, small RNA analysis, and amplicon sequencing.
A MIQE Case Study — Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data
Bio-Rad Laboratories

In this study, the minichromosome maintenance (MCM) protein, MCM7, was selected as a model target gene to investigate the importance of appropriate reference gene selection and RNA sample quality as described by the MIQE guidelines.
Sequence-Specific DNA Assay
Molecular Devices

The Threshold® system from Molecular Devices, together with the Immuno Ligand Assay (ILA) kit can be used to measure specific DNA sequences. Biotinylated or fluorescienated oligonucleotide probes, specific to the target sequence, anneal to the digested target DNA creating a probe-target hybrid which can then be detected and quantified against a standard curve, with pleasing results.
High Yield and Quality with Thermo Scientific Microtiter Deep Well 96 plates in KingFisher Flex Process
Thermo Fisher Scientific

The Thermo Scientific KingFisher Flex magnetic particle processor is specifically designed to automate the time-consuming sample preparation of proteins, nucleic acids and cells in 96-well plate formats.
Isolation and Purification of Genomic DNA and Viral RNA from Clinical Samples with Thermo Scientific KingFisher Flex
Thermo Fisher Scientific

The purification of DNA and RNA from clinical samples requires rapid and reliable processing of large quantities of samples. Automated purification of nucleic acids, proteins and cells with Thermo Scientific KingFisher Flex is a reproducible and high quality method, which produces excellent material for further analyses.
Efficient Delivery of Thermo Scientific Dharmacon SMARTpool siRNA Reagents in Difficult-to-Transfect Cell Lines Using Nucleofector® 96-well Shuttle® System
Lonza

siRNA-mediated gene knockdown is a powerful tool that has been used to identify gene function and elucidate biological pathways. Successful siRNA experiments involving knockdown of individual genes or collections of gene targets require efficient delivery of highly functional and specific siRNA molecules into appropriate cells.
ScreenTape® Enables Fast Quality Control of miRNA qPCR Reactions
AnaSpec

The ScreenTape® System, developed by Lab901, employs a unique microscale polymer tape that contains all the reagents required for DNA electrophoresis. It brings major advantages of convenience, ease of use and safety to molecular biologists as absolutely no handling of gels and buffers is required.
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