Corporate Banner
Satellite Banner
qPCR
Scientific Community
 
Become a Member | Sign in
Home>Events>This Event
  Events - October 2013


Principles, Parameters and applications of PCR

21 Oct 2013 - 21 Oct 2013 - Avans University, Breda, the Netherlands



Bookmark and Share


Aim of the course:
This short course aims at training the participant in the basic principles and applications of the Polymerase Chain Reaction (PCR) and real-time PCR. The program consists of a series lectures and experiments covering all basic PCR topics like efficiency, fidelity and specificity of the PCR reaction.

Participants:
This course is developed as an introduction to the PCR and especiall suitable as a basic introduction to our advanced course on Real-time PCR in molecular diagnostics (2548). Basic knowledge of molecular biology in general is desirable.

Course content:

Theory:
  • Principles of DNA amplification technologies; The biochemical and physical aspects of the PCR 
  • Principles, parameters, and design of (real-time) PCR primers 
  • Principles and parameters of detection of PCR products 
  • Principles of Real-time PCR; detection formats, assay formats 
  • PCR contamination prevention 
Practicals: 
  • Real-time PCR dilution series and melting curve analysis: 
  • Optimization and determination of the sensitivity of real-time PCR with SybrGreen
  • Optimization and determination of the sensitivity of real-time PCR with Hybridisation probes 
General information: 
Code: 2545 
Registration fee: € 400,- The fee includes course materials and lunch 
Language: English 
Location: Avans Hogeschool, Lovensdijkstraat 61, Breda, The Netherlands route description This course can be combined with the advanced course on Real-Time PCR in molecular Diagnostics. 
Course fee of a combined registration (2545 + 2548) is € 1.200,-


Further information
Scientific News
Analysing 10,000 Cells Simultaneously
New techniquethat traps 10,000 cells on a single chip has potential for cancer screening for individuals.
$1M NIH Grant to Refine PCR Based Cancer Test
Researchers at Cornell University, Weill Cornell Medicine, the University of California, San Francisco, and the Infectious Diseases Institute in Kampala, Uganda, recieve a four-year, $1 million grant to hone technology for a quick, in-the-field diagnosis of Kaposi's sarcoma — a cancer frequently related to HIV infections.
Genetic Tug of War Before Cells Decide Fate
Researchers report that as developing blood cells are triggered by genetic signals firing on and off, a 'tug of war' occurs.
Linkage Biosciences Awarded NHS Contract
Comapny announces that it has been awarded a four-year contract by NHS Blood and Transplant (NHSBT) in the UK for implementation of the LinkSeq™ Real-Time PCR HLA typing product.
Understanding Tumor Evolution
Study provides insight into tumor evolution; may point to improved diagnosis and treatment.
Frankfurter Fraud: Finding Out What’s In Your Hot Dog
Scientists have developed a technique to test the meat content of Frankfurters.
How Cloud Connectivity Can Combat the Reproducibility Crisis
This infographic explains the reproducibility crisis, and how cloud connectivity can help overcome this problem.
Keeping Cells in Shape to Fight Sepsis
Boosting levels of a protein that controls the shape and activity of a crucial group of white blood cells improves survival and recovery chances during sepsis.
This 3D Printer Can Detect Infectious Diseases
Researchers at AI Biosciences, Inc. have repurposed 3D printers into machines capable of performing sample preparation and DNA amplification.
Natera, UCSF Announce Collaboration
Joint research program to study DNA markers of kidney transplant rejection.
Scroll Up
Scroll Down
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!