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  Events - October 2013


Principles, Parameters and applications of PCR

21 Oct 2013 - 21 Oct 2013 - Avans University, Breda, the Netherlands



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Aim of the course:
This short course aims at training the participant in the basic principles and applications of the Polymerase Chain Reaction (PCR) and real-time PCR. The program consists of a series lectures and experiments covering all basic PCR topics like efficiency, fidelity and specificity of the PCR reaction.

Participants:
This course is developed as an introduction to the PCR and especiall suitable as a basic introduction to our advanced course on Real-time PCR in molecular diagnostics (2548). Basic knowledge of molecular biology in general is desirable.

Course content:

Theory:
  • Principles of DNA amplification technologies; The biochemical and physical aspects of the PCR 
  • Principles, parameters, and design of (real-time) PCR primers 
  • Principles and parameters of detection of PCR products 
  • Principles of Real-time PCR; detection formats, assay formats 
  • PCR contamination prevention 
Practicals: 
  • Real-time PCR dilution series and melting curve analysis: 
  • Optimization and determination of the sensitivity of real-time PCR with SybrGreen
  • Optimization and determination of the sensitivity of real-time PCR with Hybridisation probes 
General information: 
Code: 2545 
Registration fee: € 400,- The fee includes course materials and lunch 
Language: English 
Location: Avans Hogeschool, Lovensdijkstraat 61, Breda, The Netherlands route description This course can be combined with the advanced course on Real-Time PCR in molecular Diagnostics. 
Course fee of a combined registration (2545 + 2548) is € 1.200,-


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