Single-Cell Transfection Tool Enables Added Control for Biological Studies
Northwestern University researchers have developed a new method for delivering molecules into single, targeted cells through temporary holes in the cell surface.

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TATAA Strengthens Quality Assurance Program
TATAA Biocenter expands offerings using AATI’s Fragment Analyzer™ to assess RNA quality in gene expression analysis.

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Droplet Digital™ PCR Works for GMO Quantification
A study published in the journal PLOS ONE has found that Droplet Digital PCR technology is suitable for routine analysis of genetically modified organisms in food, feed and seeds.

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Single-Reaction, Multiplex, RT-PCR for the Detection, Quantitation, and Serotyping of Dengue Viruses
Article demonstrates a diagnostic test that is more analytically sensitive than a commonly used reference molecular assay.

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PCR-based Pool Screening Method for High Throughput Wuchereria Bancrofti Detection
The purpose of this study was to improve the DNA extraction protocol and qPCR assay to achieve the detection of a single mf in a pool of at least twelve 60-µl blood samples.

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New System to Improve DNA Sequencing
A sensing system developed at Cambridge is being commercialised in the UK for use in rapid, low-cost DNA sequencing.

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Detection of Novel Mutations that Cause Autosomal Dominant Retinitis Pigmentosa in Candidate Genes
In current clinical practice, sequencing of candidate genes involved in a disease in individual patient samples is becoming increasingly important.

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Development of a Neutralization Assay for Influenza Virus using an Endpoint Assessment
This study demonstrates the feasibility of using a qPCR-based assessment for measuring the neutralization of an RNA virus such as influenza virus.

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Toddler 'Functionally Cured' of HIV infection, NIH-Supported Investigators Report
Discovery provides clues for potentially eliminating HIV infection in other children.

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New Paths Explored for Curbing Genetic Malfunctions
Investigators probe mechanisms of RNA synthesis.

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Surveying Cells, One At a Time
When studying any kind of population — people or cells — averaging is a useful, if flawed, form of measurement.

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RainDance Attracts Top-Tier Commercial Leadership to Accelerate Global Growth
RainDance Technologies, Inc. has announced several high-profile additions to its commercial leadership team.

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Dr. Gerald 'Jerry' Zon Launches New Blog for TriLink BioTechnologies
Dr. Gerald Zon, noted scientist in the field of nucleic acid research recently launched a new blog in conjunction with TriLink Biotechnologies.

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VIB Inks qBase+ Group Licensing Agreement with Biogazelle
VIB to accelerate its qPCR analyses by using qbase+ as the software.

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TriLink BioTechnologies Announces Worldwide License with QuantiBact A/S
TriLink BioTechnologies, Inc. announced that it has signed a licensing agreement with QuantiBact A/S to offer twisted intercalating nucleic acid (TINA) modified oligos.

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Analytik Jena Establishes Subsidiary in Thailand
New company to bolster Analytik Jena’s activities in the Southeast Asian market.

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QIAGEN Acquires Ingenuity Systems
Suite of web-based applications powered by a unique expert-curated Knowledge Base eases major bottlenecks to leveraging genomic data in research and clinical diagnostics.

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Singapore Single-Cell Research Centre Opens Door for Asian Biological Discoveries
Centre dedicated to diagnosis and treatment derived from single-cell exploration.

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Miltenyi Biotec Acquires Owl Biomedical, Inc.
Microchip cell sorting technology broadens cell isolation capabilities.

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Global DNA Diagnostics Market is Expected to Reach Nearly $36.5 Billion by 2018
Molecular diagnostics and molecular imaging for diagnostics have benefited greatly from research conducted since the completion of the Human Genome Project.

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High-Throughput Analysis of DNA Samples using the D1K ScreenTape Assay and the Agilent 2200 TapeStation System
Agilent Technologies

Recent advances in genomics demands to look at a wealth of genetic information in a short period of time. DNA analysis using slab gel electrophoresis and capillary electrophoresis are widely being used as a QC step in next generation sequencing and microarray studies. However, often these techniques lack the speed and involve more manual steps to perform the assay.

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Hot Start dNTPs – Pushing the Limits of PCR
Agilent Technologies

Hot Start dNTPs are a distinct approach that employs modified nucleoside triphosphates with a thermolabile protecting group. This modification blocks low temperature primer extension and is released at higher temperatures to allow for more specific DNA polymerase incorporation.

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RNA Quality Control using the Agilent 2200 TapeStation System –Assessment of the RINe Quality Metric
Agilent Technologies

Here, we present a comparative study between the RINe quality score obtained from R6K ScreenTape and High Sensitivity R6K ScreenTape compared to the RIN quality metric obtained from the 2100 Bioanalyzer system.

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Simultaneous RT-qPCR Measurement of 1718 Long Non-Coding RNAs
biogazelle

Massively parallel RNA-sequencing revealed that the human genome is pervasively transcribed, resulting in the production of thousands of non-coding RNA transcripts.

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Advanced Copy Number Variant Analysis with qbasePLUS 2
biogazelle

Copy number changes under the form of deletions and duplications are known to be involved in numerous human genetic disorders. Moreover, each individual’s genome embodies several copy number polymorphisms of various sizes which are thought to contribute to normal phenotypic variation and susceptibility to multifunctional disease.

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ChIP-qPCR and qbasePLUS Jointly Identify a MYCN Activated miRNA Cluster in Cancer
biogazelle

This study applies ChIP-qPCR tp assess binding of transcription factor MYCN to miRNA cluster 17-92, to positive control target MDM2, and to a negative control target region.

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Development of an Automated Platform for HT Cloning and Expression
Novartis Vaccines and Diagnostics S.R.L.

Biomolecular protocols covering the whole cloning process were implemented in liquid handler robots. When compared to the manual approach, it was found that automation significantly speeds up HT cloning.

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Novel, Fully Automated Method Allows Efficient Analysis of qPCR Data for Qualitative Calling Based on Comparative Cq
Azure PCR Limited

Assessment to ascertain if a method for analysis of qPCR data dependent on manual intervention can be replaced by automated analysis using the AzurePCRTM method.

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Volume-Related Inhibitors Standardization for Reverse Transcription Quantitative Polymerase Chain Reaction Experiments
IRBA

This poster addresses the reliability of qPCR data and its dependence on technical variations. The proposal is that constant volume of RNA extract can improve reliability of RT-qPCR.

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Defining off-target cleavage in a pair of Zinc Finger Nucleases
University of Utah

This study looks at off-target cleavage of Zinc Finger Nucleases (ZNFs) in Drosophila in an attempt to analyze potential cleavage spots, with a view to designing more efficient ZFNs.

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Rapid, High Throughput Extraction and Purification of Genomic DNA from Whole Blood Using the Akonni TruTip® Extraction System on the Hamilton Microlab® STAR Liquid Handling System
Hamilton

This application note demonstrates the high precision of the automated extraction system in processing genomic DNA from low volume whole blood samples and its advantage over competitors.

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Automated GS FLX Titanium emPCR Enrichment using the REM e System on a Hamilton Microlab® STARlet Liquid Handler
Hamilton

The Genome Sequencer FLX System is a versatile sequencing platform suitable for a wide range of research applications, including de novo sequencing and assembly of genomic DNA, transcriptome sequencing, small RNA analysis, and amplicon sequencing.

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A MIQE Case Study — Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data
Bio-Rad Laboratories

In this study, the minichromosome maintenance (MCM) protein, MCM7, was selected as a model target gene to investigate the importance of appropriate reference gene selection and RNA sample quality as described by the MIQE guidelines.

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Sequence-Specific DNA Assay
Molecular Devices

The Threshold® system from Molecular Devices, together with the Immuno Ligand Assay (ILA) kit can be used to measure specific DNA sequences. Biotinylated or fluorescienated oligonucleotide probes, specific to the target sequence, anneal to the digested target DNA creating a probe-target hybrid which can then be detected and quantified against a standard curve, with pleasing results.

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High Yield and Quality with Thermo Scientific Microtiter Deep Well 96 plates in KingFisher Flex Process
Thermo Fisher Scientific

The Thermo Scientific KingFisher Flex magnetic particle processor is specifically designed to automate the time-consuming sample preparation of proteins, nucleic acids and cells in 96-well plate formats.

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Isolation and Purification of Genomic DNA and Viral RNA from Clinical Samples with Thermo Scientific KingFisher Flex
Thermo Fisher Scientific

The purification of DNA and RNA from clinical samples requires rapid and reliable processing of large quantities of samples. Automated purification of nucleic acids, proteins and cells with Thermo Scientific KingFisher Flex is a reproducible and high quality method, which produces excellent material for further analyses.

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Efficient Delivery of Thermo Scientific Dharmacon SMARTpool siRNA Reagents in Difficult-to-Transfect Cell Lines Using Nucleofector® 96-well Shuttle® System
Lonza

siRNA-mediated gene knockdown is a powerful tool that has been used to identify gene function and elucidate biological pathways. Successful siRNA experiments involving knockdown of individual genes or collections of gene targets require efficient delivery of highly functional and specific siRNA molecules into appropriate cells.

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ScreenTape® Enables Fast Quality Control of miRNA qPCR Reactions
AnaSpec

The ScreenTape® System, developed by Lab901, employs a unique microscale polymer tape that contains all the reagents required for DNA electrophoresis. It brings major advantages of convenience, ease of use and safety to molecular biologists as absolutely no handling of gels and buffers is required.

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Advanced Software for Real-Time PCR Data Analysis by Integromics
Integromics

Got data? Get answers! Real-Time StatMiner™ is workflow oriented: a step-by-step guide to ensure reliable and accurate results using powerful statistics and interactive visualizations. Average time of analysis for all skill level users- just 10 minutes! Steps of the analysis consist of importing raw Ct data, performing quality control and outlier detection, examining correlation between biological replicates, selecting the optimal combination of endogenous controls for normalization based on sta

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Identify optimal reference genes for realtime-qPCR with GenEx MultiD Analyses AB
MultiD Analyses AB

Tailor-made for qPCR. Powerful. Easy to use. GenEx is a popular software for qPCR data processing and analysis. Built in a modular fashion GenEx provides a multitude of functionalities for the qPCR community, ranging from basic data editing and management, such as data pre-processing and group assignments, to advanced cutting-edge data analysis, such as PCA and ANN.

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