Satellite Banner
qPCR
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Correction of RT–qPCR Data for Genomic DNA-derived Signals with ValidPrime

Published: Monday, January 09, 2012
Last Updated: Monday, January 09, 2012
Bookmark and Share
Henrik Laurell et al propose a novel method (ValidPrime) that is more accurate than traditional RT(_) controls to test qPCR assays with respect to their sensitivity toward gDNA by compensating for DNA background.

Genomic DNA (gDNA) contamination is an inherent problem during RNA purification that can lead to non-specific amplification and aberrant results in reverse transcription quantitative PCR (RT—qPCR).

ValidPrime measures the gDNA contribution using an optimized gDNA-specific ValidPrime assay (VPA) and gDNA reference sample(s). The VPA, targeting a nontranscribed locus, is used to measure the gDNA contents in RT(+) samples and the gDNA reference is used to normalize for GOI-specific differences in gDNA sensitivity.

This article demonstrates that the RNA-derived component of the signal can be accurately estimated and deduced from the total signal. ValidPrime corrects with high precision for both exogenous (spiked) and endogenous gDNA, contributing _60% of the total signal, whereas substantially reducing the number of required qPCR control reactions. In conclusion, ValidPrime offers a cost-efficient alternative to RT(_) controls and accurately corrects for signals derived from gDNA in RT–qPCR.

To read the full article, please click on the link provided.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,800+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Polar Distribution of MicroRNAs Discovered within Eukaryotic Cells
A plausible new mechanism contributing to asymmetric cell division.
Thursday, June 11, 2015
TATAA Partners with Qlucore
TATAA Biocenter introduces partnership with Qlucore to use Qlucore Omics Explorer in its Next Generation Sequencing courses and client services.
Monday, March 09, 2015
TATAA Biocenter Receives French Ministry Approval
Approval makes the Company even more competitive on the French market.
Tuesday, August 12, 2014
TATAA Biocenter Awarded for Best-in-Class Gene Expression Profiling Services
Numerous accreditations from diverse institutes and customer validations lend credibility to its educational programs.
Wednesday, August 21, 2013
TATAA Strengthens Quality Assurance Program
TATAA Biocenter expands offerings using AATI’s Fragment Analyzer™ to assess RNA quality in gene expression analysis.
Tuesday, May 21, 2013
TATAA Biocenter Introduces Ingenuity Systems iReport into qPCR Workflow
TATAA Biocenter, Europe´s leading provider of genomic services using quantitative real-time polymerase PCR (qPCR), is complementing its offerings in high-throughput gene expression profiling with Ingenuity® iReport™.
Wednesday, January 23, 2013
Internship Opening with the Edu-GLIA project
Through the Initial Training Network (ITN) funded by the European Commission under the Seventh Framework Programme (FP7) TATAA Biocenter has been awarded early stage research positions within the Edu-GLIA project.
Monday, October 19, 2009
TATAA Biocenter Scientists Pioneer 3-Way Expression Profiling
A TATAA Biocenter researcher has performed a 3-way expression profiling study of the metabolism of Saccharomyces cerevisiae also called baker’s yeast.
Friday, April 25, 2008
TATAA Biocenter Establishes Centre in Prague, Czech Republic
TATAA Biocenter in Prague will offer full range of real-time PCR services including high throughput real-time PCR analysis and contract research.
Monday, April 23, 2007
Scientific News
Surprising Mechanism Behind Antibiotic-Resistant Bacteria Uncovered
Now, scientists at TSRI have discovered that the important human pathogen Staphylococcus aureus, develops resistance to this drug by “switching on” a previously uncharacterized set of genes.
Researchers Develop qPCR Prognosis Test for NSCLC Patients
A nine-gene molecular prognostic index (MPI) for patients with early-stage non-small cell lung cancer (NSCLC) was able to provide accurate survival stratification and could potentially inform the use of adjuvant therapy in patients struggling with the disease.
Genome Wide Annotation of Primary miRNAs Reveals Novel Mechanisms
Researchers have devised a strategy for genome-wide annotation of primary miRNA transcripts, providing extensive new annotations in human and mouse, and shedding light on mechanisms of regulation of microRNA gene expression.
‘Fishing Expedition’ Nets Nearly Tenfold Increase in Number of Sequenced Virus Genomes
Newly developed computational tool finds 12,500 genomes of viruses that infect microbes.
First Gene that Causes Mitral Valve Prolapse Identified
An international research collaboration led by MGH investigators has identified the first gene in which mutations cause the common form of mitral valve prolapse, a heart valve disorder that affects almost 2.5 percent of the population.
Automation Abound at AACC in Atlanta
Discover the latest breakthroughs, trends and products from the AACC Annual Meeting & Clinical Lab Expo.
Ultrafast DNA Diagnostics
New technology developed by UC Berkeley bioengineers promises to make a workhorse lab tool cheaper, more portable and many times faster by accelerating the heating and cooling of genetic samples with the switch of a light.
The Genetic Roots of Adolescent Scoliosis
Scientists at the RIKEN Center for Integrative Medical Sciences in collaboration with Keio University in Japan have discovered a gene that is linked to susceptibility of Scoliosis.
Diagnostic Test Developed for Enterovirus D68
researchers at Washington University School of Medicine in St. Louis have developed a diagnostic test to quickly detect enterovirus D68 (EV-D68), a respiratory virus that caused unusually severe illness in children last year.
Simple Technology Makes CRISPR Gene Editing Cheaper
University of California, Berkeley, researchers have discovered a much cheaper and easier way to target a hot new gene editing tool, CRISPR-Cas9, to cut or label DNA.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!