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Reliable and Rapid Characterization of Functional FCN2 Gene Variants Reveals Diverse Geographical Patterns

Published: Monday, May 21, 2012
Last Updated: Monday, May 21, 2012
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In this study, scientists genotyped and characterized four FCN2 functional SNP variants in West Africans, South Americans, South East Asians from Vietnam and Caucasians from Germany using an available rapid, reliable and cost effective FRET-RTPCR methodology.

Background:
Ficolin-2 coded by FCN2 gene is a soluble serum protein and an innate immune recognition element of the complement system. FCN2 gene polymorphisms reveal distinct geographical patterns and are documented to alter serum ficolin levels and modulate disease susceptibility.

Methods:
We employed a real-time PCR based on Fluorescence Resonance Energy Transfer (FRET) method to genotype four functional SNPs including -986 G > A (#rs3124952), -602 G > A (#rs3124953), -4A > G (#rs17514136) and +6424 G > T (#rs7851696) in the ficolin-2 (FCN2) gene. We characterized the FCN2 variants in individuals representing Brazilian (n = 176), Nigerian (n = 180), Vietnamese (n = 172) and European Caucasian ethnicity (n = 165).

Results:
We observed that the genotype distribution of three functional SNP variants (986 G > A, - 602 G > A and -4A > G) differ significantly between the populations investigated (p <0.0001). The SNP variants were highly linked to each other and revealed significant population patterns. Also the distribution of haplotypes revealed distinct geographical patterns (p < 0.0001).

Conclusions:
The observed distribution of the FCN2 functional SNP variants may likely contribute to altered serum ficolin levels and this may depend on the different disease settings in world populations. To conclude, the use of FRET based real-time PCR especially for FCN2 gene will benefit a larger scientific community who extensively depend on rapid, reliable method for FCN2 genotyping.

This article is published online in BMC Medical Genetics and is free to access.


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