Corporate Banner
Satellite Banner
qPCR
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Improving Reproducibility in Real-Time PCR

Published: Tuesday, February 19, 2013
Last Updated: Tuesday, February 19, 2013
Bookmark and Share
Improved reproducibility and productivity by using VIAFLO 96 handheld electronic pipette.

INTEGRA has reported how a leading US clinical diagnostic laboratory has, using a VIAFLO 96 handheld electronic pipette, significantly improved the reproducibility and productivity with which it carries out Real-time PCR assays.

An important source of income for the clinical diagnostic lab is the screening of patient samples for the presence of disease markers by gene expression analysis.

This analysis involved transferring pre-treated patient samples from a 96-well plate source to a target PCR plate, adding the PCR master mix and then subsequently analyzing the plate by real-time PCR.

The lab traditionally processed around 100 plates per day and the work was all done using 12-channel pipettes.

Manually filling a 96-well plate, including changing of pipette tips after every sample transfer, was acknowledged to be a time consuming task and transferring the samples often proved to be prone to error.

Looking to improve this process the lab invested in an INTEGRA VIAFLO 96 handheld electronic pipette to both load the patient samples into the plates and then to transfer the PCR master mix to the reaction plate.

As a result - the work is now done in half the time. More important to the clinical diagnostic lab are two additional improvements.

Transfer mistakes from plate to plate have been eliminated and now the light-sensitive PCR master mix is only exposed to daylight for a few seconds prior to the reaction, as all plate wells can be processed at the same time.

According to the lab manager, the VIAFLO 96 has increased accuracy, robustness and precision of the assay in comparison to their previous use of manual 12 channel pipettes.

The INTEGRA VIAFLO 96 is a handheld 96-channel electronic pipette that enables fast, precise and easy simultaneous transfer of 96 samples.

Customers will immediately benefit from the increased productivity delivered by their VIAFLO 96. Fast replication or reformatting of 96 and 384 well plates and high precision transferring of reagents, compounds and solutions to or from microplates is now as easy as pipetting with a standard electronic pipette into a single tube.

Four pipetting heads with pipetting volumes up to 12.5 µl, 125 µl, 300 µl or 1250 µl are available for the VIAFLO 96. These pipetting heads are interchangeable within seconds enabling optimal matching of the available volume range to the application performed.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,100+ scientific posters on ePosters
  • More than 4,500+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
Finding Zika One Paper Disc At A Time
A novel, inexpensive method for detecting the Zika virus could help slow spread of outbreak, and potentially other future pandemic diseases.
Rapid Diagnosis of Bacterial Infections
Mass. General-developed compact system could shorten diagnostic time from days to hours, bring testing to point of care.
Monovar Drills Down Into Cancer Genome
Rice, MD Anderson develop program to ID mutations in single cancer cells.
Biomarkers for Profiling Prostate Cancer Patients
Exiqon A/S has announced the publication of validation of prognostic microRNA biomarkers for the aggressiveness of prostate cancer in independent cohorts.
Gene Analysis System Could Accelerate Pace of Research on the Space Station
The WetLab-2 system will enable spaceflight genomic studies involving a wide variety of biospecimen types in the unique microgravity environment of space.
Liquid Biopsy for NSCLC
'Liquid biopsy' blood test accurately detects key genetic mutations in most common form of lung cancer, study finds.
Rare DNA Will Have Nowhere To Hide
Two National Institutes of Health grants back Rice University effort to develop new diagnostics.
Seeing Cell to Cell Differences
Seeing differences between individual cells for first time explains symptoms of rare genetic disorders, finds penn study.
Circular RNAs Implicated in Cancer
Circular RNAs – like their protein counterparts – are also affected by genomic rearrangements in cancer, resulting in abnormal fusions.
Know Thy Helicobacter pylori
Non-invasive method for genotyping H.pylori developed.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,100+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!