Satellite Banner
Scientific Community
Become a Member | Sign in
Home>News>This Article

New Cyanine Dyes Reduce Time and Cost of Oligo Labelling

Published: Monday, March 25, 2013
Last Updated: Sunday, March 24, 2013
Bookmark and Share
Link Technologies’ solid-supported Cyanine 540 and 650 dyes increase workflow efficiency.

Link Technologies Ltd has launched a range of high quality cyanine dye products to support the continued increase in demand for fluorescent labelling in biological imaging and molecular diagnostics.

As well as introducing a new range of 3’-CPG solid supports modified with Cyanine 540 and Cyanine 650, Link Technologies now also manufactures and supplies the commonly used phosphoramidites, so researchers can now source an extended range of cyanine reagents produced to Link Technologies’ exacting standards.

Until now, 3’-cyanine modification of an oligonucleotide could only be done post-synthetically, normally to an amino-modified oligo, or by the use of a cyanine phosphoramidite onto a universal or modified support that will not interfere with the intended use of the oligo.

With Link Technologies’ innovative 3’-modified 1000Å CPG supports, customers can add cyanine dyes directly to their oligos of interest without any additional modification step.

Link Technologies’ cyanine dye supports therefore save time by reducing the number of experimental steps and avoid the unnecessary cost of amino-modified oligos or the inconvenience of combining cyanine phosphoramidites and universal supports.

Used as fluorescent markers in oligonucleotide synthesis, dyes are central to numerous detection techniques, such as probes for monitoring real-time PCR, fluorescence in situ hybridization, SERRS-based DNA detection assays and FRET studies in general (e.g. DNA-protein interaction studies).

3’-Labelling is particularly useful in the latter where the FRET partner is incorporated either at the 5’-end or within the oligonucleotide sequence.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,600+ scientific posters on ePosters
  • More than 3,800+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Link Technologies Secures Exclusive License for Ultra Sensitive Diagnostic Probes
Deal marks the start of ongoing collaboration between Link and The University of Manchester.
Tuesday, December 01, 2009
Link Technologies Inks Exclusive License to Agilent Technologies’ TC RNA Monomers
Under the terms of the agreement Link will manufacture and sell the four RNA monomer phosphoramidite reagents for use in research.
Monday, May 18, 2009
Scientific News
New Virus Identified In Blood Supply
Scientists have discovered a new virus that can be transmitted through the blood supply.
Tracking Changes in DNA Methylation In Real Time At Single-Cell Resolution
Whitehead Institute researchers have developed a methodology to monitor changes in DNA methylation over time in individual cells.
DNA Alterations as Among Earliest to Occur in Lung Cancer Development
Genetic footprints of precancer detectable in some blood samples.
Bar-Coding Technique Opens Up Studies Within Single Cells
Scientists have developed a method for simultaneously imaging and identifying dozens of molecules within individual cells.
Scientists Learn How to Predict Plant Size
VIB and UGent scientists have developed a new method which allows them to predict the final size of a plant while it is still a seedling.
New Device Reduces Time to Diagnose Infections
A new diagnostic device created by a collaborative team of UA engineers and scientists may significantly reduce the amount of time necessary to diagnose tissue infections.
Surprising Mechanism Behind Antibiotic-Resistant Bacteria Uncovered
Now, scientists at TSRI have discovered that the important human pathogen Staphylococcus aureus, develops resistance to this drug by “switching on” a previously uncharacterized set of genes.
Researchers Develop qPCR Prognosis Test for NSCLC Patients
A nine-gene molecular prognostic index (MPI) for patients with early-stage non-small cell lung cancer (NSCLC) was able to provide accurate survival stratification and could potentially inform the use of adjuvant therapy in patients struggling with the disease.
Genome Wide Annotation of Primary miRNAs Reveals Novel Mechanisms
Researchers have devised a strategy for genome-wide annotation of primary miRNA transcripts, providing extensive new annotations in human and mouse, and shedding light on mechanisms of regulation of microRNA gene expression.
‘Fishing Expedition’ Nets Nearly Tenfold Increase in Number of Sequenced Virus Genomes
Newly developed computational tool finds 12,500 genomes of viruses that infect microbes.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos