Corporate Banner
Satellite Banner
qPCR
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Discussing the Science of Big DNA

Published: Monday, September 23, 2013
Last Updated: Monday, September 23, 2013
Bookmark and Share
Read about the most recent advances in synthetic biology in the latest issue of DECODED.

In the latest issue of DECODED, the quarterly newsletter from Integrated DNA Technologies (IDT), Dr Dan Gibson discusses the science of big DNA. Dr Gibson spent most of the last decade working at the J Craig Venter Institute, as well as Synthetic Genomics Inc. (SGI), to develop, establish, and commercialize methods to take synthetic biology beyond its current limitations. In 2010 Dr Gibson and his team produced a DNA construct of 1.1 Mb, the largest synthetic DNA sequence to date, something which had not been achievable using traditional restriction/ligation methodologies. Instead, the team developed the Gibson Assembly™ Method, which uses a unique enzyme mix to assemble DNA elements with 20–80 overlapping bases. Once reamplified, the population, enriched with the correct sequence, is much faster to screen. The technology makes possible the creation of 10–30 kb sequences—the size of an entire biological pathway—in less than a week, and without cloning into E. coli. Dr Gibson and his team are currently looking at ways in which they can refine DNA assembly methods; improve large gene delivery to host cells; and develop methods that rely less on host organisms for copying their synthetic constructs.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 4,000+ scientific posters on ePosters
  • More than 5,300+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

IDT and Ubiquitome Partner to Develop Mobile Ebola Test
Handheld, battery-powered Freedom4 enables rapid field deployment of gold standard molecular test.
Wednesday, December 03, 2014
Novel rhPCR Unravels Gene Expression
Method enables identification and analysis of highly similar alternatively spliced transcripts.
Tuesday, September 09, 2014
IDT Shares Comprehensive qPCR Resources
Special qPCR compendium issue of DECODED now freely available.
Wednesday, August 06, 2014
IDT Acquires SURVEYOR® Nuclease Business from Transgenomic
Addition of SURVEYOR enzyme and kits expands IDT's molecular biology product offerings.
Wednesday, July 02, 2014
Webinar: the Cutting-edge qPCR Approach to Single-cell Expression Profiling
Join IDT and Professor Mikael Kubista for insight into pioneering qPCR methods, June 25.
Monday, June 24, 2013
Insightful Advice for Avoiding Clonal Deletions
The latest IDT DECODED newsletter provides recommendations for successful DNA cloning.
Friday, November 09, 2012
IDT Supports the qPCR Community
Recent qPCR webinar and application guide advance end-user knowledge.
Tuesday, June 26, 2012
IDT Presents at Presidential Commission for the Study of Bioethical Issues
Representing the International Gene Synthesis Consortium and its ongoing work to prevent the misuse of synthetic genes.
Tuesday, September 21, 2010
IDT Signs Contract to Supply Research Councils UK with Custom Oligonucleotides
IDT’s expert production technologies to aid RCUK in delivering on the UK government’s objectives for science and innovation.
Tuesday, July 13, 2010
IDT Shares its Expertise at qPCR 2010 in Vienna
Dr Mark Behlke, IDT’s Chief Scientific Officer will talk about, ‘Hydrolysis probes with improved quenching and performance’.
Friday, April 09, 2010
Scientific News
Top 10 Life Science Innovations of 2016
2016 has seen the release of some truly innovative products. To help you digest these developments, The Scientist have listed their top picks for the year.
Using Cancer Cells' Mass to Predict Treatment Response
A device has been developed that can detect changes in cell mass at a minute scale.
NVIDIA Awards $400k to Trailblazers in Cancer Research
NVIDIA Foundation furthers research that could lead to new and more targeted treatments with investments.
Malaria Parasite Evades Rapid Test Detection in Children
A study at the University of North Carolina found that gene deletion poses a threat to Malaria eradication efforts.
Working With Advanced Nanoimagers
Oxford Nanoimaging report on the work of early-adopters for their Nanoimager technology at the MRC Centre for Molecular Bacteriology and Infection.
History of Cells Told Through MEMOIR
MEMOIR technique developed by CalTech researchers enables the life history of cells to be read.
Nanoscale ‘Muscles’ Powered by DNA
Scientists have developed nanoscale "muscles" to integrate with custom DNA, that can force the material to bend, curl and flip.
Dissecting Bacterial Infections at the Single-Cell Level
Researchers have used single-cell analysis technology to provide new insight into the Salmonella infection process.
Peer Review is in Crisis, But Should be Fixed, Not Abolished
After the time to get the science done, peer review has become the slowest step in the process of sharing studies, and some scientists have had enough.
Cancer Stem Cells Fuel Tumor Growth
Mass. General, Broad Institute team finds strong evidence that cancer stem cells are important drivers of tumors in patients.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
4,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,300+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!