Promega Corporation has announced the launch of the Maxwell® 16 LEV RNA FFPE Kit for RNA isolation from mammalian FFPE (formalin fixed paraffin embedded) tissue samples.
The new kit is designed to rapidly extract pure, high yield RNA from thin sections of FFPE tissue samples, with consistency and time saving provided by the automated Maxwell 16 instrument.
The purified RNA is suitable for downstream applications including RT-PCR, RT-qPCR, endpoint RT-PCR, cDNA library synthesis and gene expression analysis.
Specifically designed for optimal purification of RNA from 5µm sections of FFPE samples, the new kit maximizes RNA yield and eliminates the need to concentrate the extract prior to amplification, minimizing sample waste and the need for reruns.
The kit is very simple to use, with the automated walkaway system able to extract from 16 samples in a single run. Lysates are placed directly into the cartridges, and RNA is ready for amplification in approximately 60 minutes (after proteinase K digest).
The new kit does not use organic solvents such as xylene, thereby providing a much safer method than other available kits, and quality testing shows there are virtually no PCR inhibitor contaminants in purified samples.
“Molecular pathology and oncology labs are regularly required to extract RNA from FFPE samples. The new kit from Promega simplifies and automates this process whilst offering the high yield and purity needed for downstream applications, saving researchers significant time and offering the potential to improve results,” commented Jeff Franz, Global Product Leader, Promega corporation.