Corporate Banner
Satellite Banner
qPCR
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Iowa State University Researcher Develops Software that Sidesteps Effects of qPCR-Inhibitory Materials

Published: Wednesday, March 21, 2007
Last Updated: Wednesday, March 21, 2007
Bookmark and Share
The software system PREXCEL-Q can provide a way to detect and avoid inhibition, and enables investigators to consistently design qPCR reactions.

An Iowa State University researcher has developed a Microsoft Excel-based system that provides a way to detect and avoid inhibition, and enables investigators to consistently design dynamically sound, truly LOG-linear qPCR reactions.

PREXCEL-Q was created by Jack Gallup, assistant scientist in veterinary pathology. The description of the invention was published in the Sept. 15, 2006 issue of Biological Procedures On-line.

PREXCEL-Q circumvents the effect of qPCR-inhibitory material in all qPCR assays by exactly calculating the safe (non-inhibitory) dilutions for all samples and standards (without over-diluting any of them) thereby allowing the assay to achieve its hallmark desired LOG-linear amplifications that are truly reflective of the absolute and/or relative abundance of the nucleic acid targets of interest in the samples. It does so by first defining the legitimate boundaries of each qPCR assay by examining representative mixtures of all samples in each assay beforehand.

The information gathered from this preliminary test allows the extent of qPCR-inhibitory behavior introduced by the samples themselves to be measured and subsequently avoided by strategic dilutions of each sample preceding final qPCR evaluation.

The program has been used at Iowa State University for the past seven years in Dr. Mark Ackermann's laboratory, and is being beta-tested by several Iowa State researchers.

PREXCEL-Q allows scientists to unveil the absolute or relative presence of any stretch of genetic material of interest in any biological sample. The program swiftly and calculates all set-up parameters for all types of qPCR set-ups, and ensures that the gene expression analyses ultimately accomplished by the technique are precise.

PREXCEL-Q can solve problems universal to all qPCR reactions, and performs all necessary qPCR set-up calculations in less than 15 minutes for up to seven qPCR targets and 72 samples at a time-calculation.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,900+ scientific posters on ePosters
  • More than 4,200+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
New Method Promises to Speed Development of Food Crops
A new study addresses a central challenge of transgenic plant development: how to reliably evaluate whether genetic material has been successfully introduced.
Study Validates Analysis of Copy Number Variation in Miniaturized Reaction Volumes
Data shows that accurate and reproducible CNV results can be produced with IntelliQube using the Array Tape® consumable.
Faster Drug Discovery?
Startup develops more cost-effective test for assessing how cells respond to chemicals.
Edited Stem Cells Offer Hope of Precision Therapy for Blindness
Findings raise the possibility of treating blinding eye diseases using a patient's own corrected cells as replacement tissue.
Parallel Single-Cell Profiling
New single-cell genomics protocol allows researchers to study links between DNA modifications (methylation) and the activity of a gene.
Pathogens Found in Iceman's Gut
Scientists discover Helicobacter pylori in the contents of Ötzi’s stomach along with some unexpected insights into the coexistence of man and bacterium.
Diagnosing Cancer from a Single Drop of Blood
What if a physician could effectively diagnose cancer from one drop of a patient’s blood?
Tracing a Cellular Family Tree
New technique allows tracking of gene expression over generations of cells as they specialize.
Accelerating Protein Evolution
A new tool enables researchers to test millions of mutated proteins in a matter of hours or days, speeding the search for new medicines, industrial enzymes and biosensors.
Ancient Viral Molecules Essential for Human Development
Genetic material from ancient viral infections is critical to human development, according to researchers at the Stanford University School of Medicine.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!