Bio-Rad Laboratories, Inc. has announced the availability of two bulletins (numbers 5713 and 5692) describing a major study demonstrating how real-time quantitative PCR (rt-qPCR) analysis provides method for evaluating the knockdown efficiency and functional consequences of RNAi-mediated gene silencing.
The study uses examples from neuroblastoma research conducted at Bio-Rad and by researchers at Ghent University, Belgium.
Real-time quantitative PCR is the method of choice for specific quantitation of nucleic acid sequences. Applications of this technology are numerous, both in molecular diagnostics and in virtually all fields of life sciences, including gene expression profiling, measurement of DNA copy number alterations, genotyping, mutation detection, pathogen detection, measurement of viral load, disease monitoring, and assessment of drug response.
Several ingredients are essential to the completion of an rt-qPCR assay, such as careful primer design and evaluation, template preparation, the use of a normalization strategy, and data analysis.
Bulletin 5713 describes the workflow steps needed for successful neuroblastoma experimentation, from experimental design to analysis of an rt-qPCR assay through careful attention to primer design and evaluation, template preparation, normalization strategy, and data analysis. Bulletin 5692 provides additional information on the experiments that contributed to research number 5713, including instruments, reagents, and procedures.
Future neuroblastoma studies are planned to evaluate results achieved using additional cell lines and varying combinations of multiple siLentMer™ siRNA duplexes, durations of effect, and concentrations of active siLentMer siRNA duplexes.