Bio-Rad Updates a Previously Published Bulletin Describing the Effect of RNA Degradation on Data Quality
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Bio-Rad Laboratories, Inc. has announced that bulletin number 5452, describing the effect of RNA degradation on data quality in quantitative PCR and microarray experiments, has been updated with new data. The paper discusses the use of automated electrophoresis in determining RNA quality prior to gene expression analysis.
Quantitative expression levels, when determined by qPCR, decrease as RNA becomes increasingly degraded. This can lead to erroneous conclusions regarding the levels of gene expression when comparing samples that are degraded to different extents. Genes with high levels of expression can still be characterized, but degradation compromises the ability to detect differences in genes that are expressed at low levels.
Bio-Rad’s Experion™ automated electrophoresis system is effective for determining the quality and quantity of RNA in gene expression analysis experiments. The Experion system quantitates and evaluates the integrity of samples using as little as 200 pg total RNA, which is several hundred times less material than that required for gel electrophoresis.
Once RNA quantity and quality have been established, a researcher can determine whether or not to move forward with additional experiments, knowing to what degree the results may be compromised.
The six-page paper provides a detailed introduction, methods description, results, and a discussion, supported by numerous graphs, tables, figures, and electropherograms.
