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Single Cell Whole Genome Amplification
GE Healthcare

The analysis of genomes at the single cell level offers unprecedented biological insights in diverse fields such as cancer research, immunology & microbiology. This poster discusses the a technology for amplification of genomic DNA form a single cell, that provides utmost sensitivity, accuracy & robustness. GE Healthcare Life Sciences had previously developed method of multiple strand displacement amplification (MDA) by Phi29 DNA polymerase

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System-Specific Periodicity in qPCR Data and its Impact on Quantitation
Joel Tellinghuisen,1 Stefan Roediger,2 Thomas Volksdorf,3 and Andrej-Nikolai Spiess3

In some commercial qPCR instruments, the fluorescence signal shows reaction-to-reaction periodicity in all cycles, and this translates into periodicity in threshold-based Cq markers.

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A Microwave/Microfluidics Integrated Label-Free Detection and Content Sensing System for Point-of-Care Applications
Gurkan Yesiloz, Muhammed S. Boybay, Carolyn L. Ren

This poster presents a droplet-based microfluidic device integrated with a microwave wave sensor, which is also a heater, for simultaneous sensing and heating of individual nanoliter-sized droplets.

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Rapid Detection of Somatic Mutations in Cancer Genes
Michael J. Powell et. al.,

Rapid higly sensitive detection of tumor gene mutations in DNA derived from FFPE or plasma samples can be achieved with QClamp PCR technology.

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Accurate Normalization of Grain Samples Using Pressure-Based Volume Measurement Technology
Bill Gigante, John Thomas Bradshaw, Tanya Knaide

A common challenge experienced by sample screening facilities is identifying individual sample quantity prior to DNA extraction. Accurately identifying sample quantity allows the user to pipette exact amounts of extraction buffer into each well.

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DHPLC Technology as a High-throughput Detection of Mutations in a Durum Wheat TILLING Population
Colasuonno P.1, Incerti O. 1, Lozito M.L. 1, Sbalzarini M. 2, Zaccagna P. 2, Papadimitriou S. 2, Blanco A. 1, Gadaleta A. 1

This study is a beautiful example of DHPLC technology application and shows an alternative tool to current strategies of SNP detection based on genotyping array.

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IDENTIFICATION AND DIFERENTIATION OF Verticillium SPECIES WITH PCR MARKERS AND SEQUENCING OF ITS REGION
Taja Jesenicnik, Nataša Štajner, Jernej Jakše, Sebastijan Radišek and Branka Javornik

The genus Verticillium is a group of ascomycete fungi, including plant-pathogenic species capable of affecting the vasculature of many agricultural crops, and therefore causes major economic losses worldwide. In 2011, a new taxonomic classification of the genus was proposed, which is now referred to as Verticillium sensu stricto, comprising ten species: V. dahliae V. albo-atru, V. alfalfae, V. longisporum, V. nonalfalfae, V. tricorpus, V. zaregamsianum, V. nubilum, V. isaacii and V. klebahnii. <

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Expression Profiling of Circulating Tumor Cells: a Prognostic and Predictive Biomarker in Cancer.
Mikael Kubista, Robert Sjöback,Marie Jindrichova, Eva Rohlova, Vendula Novosadová, Siegfrid Hauch, Bahriye Aktas, Mitra Tewes,Maren Bredemeier, and SabineKasimir-Bauer

We show non-responders to treatment among breast cancer patients can be identified by expression profiling of circulating tumor cells

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Detection in Environmental Samples of Enteroagregative Escherichia coli, stx-producing Escherichia coli and stx-converting Bacteriophage by RT- PCR: Preliminary Data
Mura Elia, Noli Alessia Caterina, Rossi Maria Lucia, Terrosu Giovanni, Fadda Antonio

The aim of this work was to collect epidemiological information concerning the presence of Enteroaggregative Escherichia coli, stx-producing Escherichia coli and stx2a-converting bacteriophage, in ruminants faeces.

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Effect of exposure to stress conditions on propidium monoazide (PMA) - qPCR based enumeration of Campylobacter in broiler carcass rinses
A. Duarte1,2*, N. Botteldoorn2 ,W. Coucke2, S. Denayer2, K. Dierick2, and M. Uyttendaele1

Evaluation and comparison of a developed PMA-qPCR Campylobacter enumeration method with the reference method, after exposure of the broiler rinse artificially contaminated with the bacteria to stress conditions.

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Showing Results 1 - 10 of 95
Scientific News
Seeing the Action
Researchers have developed a way to observe both the forces present and the behavior that occurs during cell hemifusion; the merging of the lipid bi-layers of two cells.
From Worker to Queen at the Drop of a Gene
Biologists have found that the hierarchical fate of one of nature's most important pollinators comes down to which genes are "turned on or off".
Faster, Portable Microbial analysis
New miniaturized microbial analysis machine permits the detection of microbes in water, soil and the upper atmosphere.
Genetic Markers for Detecting and Treating Ovarian Cancer
Custom bioinformatics algorithm identifies human mRNAs that distinguish ovarian cancer cells from normal cells and provide new therapeutic targets
Single-cell Analysis Hits its Stride
Advances in technology and computational analysis enable scale and affordability, paving the way for translational studies.
New Genomic Research Amends Earlier Triple Negative Breast Cancer Finding
Previously reported molecular finding unable to be validated.
LifeCodexx Reports Successful Proof-of-Principle of Innovative PCR-based NIPT Assay
Breakthrough potential as affordable NIPT solution on global basis.
Blood Test Trumps Accuracy of Standard Screening in Detecting Down Syndrome in Early Pregnancy
A blood test undertaken between 10 to 14 weeks of pregnancy may be more effective in diagnosing Down syndrome and two other less common chromosomal abnormalities than standard non-invasive screening techniques.
Highly Sensitive Detection Of Malaria Parasites
New assays can detect malaria parasites in human blood at very low levels and might be helpful in the campaign to eradicate malaria, reports a study published in PLOS Medicine.
Selection of Reference Genes for Quantitative RT-PCR Analysis
The expression stability of 12 commonly used reference genes was analysed.
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