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Single Cell Whole Genome Amplification
GE Healthcare

The analysis of genomes at the single cell level offers unprecedented biological insights in diverse fields such as cancer research, immunology & microbiology. This poster discusses the a technology for amplification of genomic DNA form a single cell, that provides utmost sensitivity, accuracy & robustness. GE Healthcare Life Sciences had previously developed method of multiple strand displacement amplification (MDA) by Phi29 DNA polymerase

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System-Specific Periodicity in qPCR Data and its Impact on Quantitation
Joel Tellinghuisen,1 Stefan Roediger,2 Thomas Volksdorf,3 and Andrej-Nikolai Spiess3

In some commercial qPCR instruments, the fluorescence signal shows reaction-to-reaction periodicity in all cycles, and this translates into periodicity in threshold-based Cq markers.

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A Microwave/Microfluidics Integrated Label-Free Detection and Content Sensing System for Point-of-Care Applications
Gurkan Yesiloz, Muhammed S. Boybay, Carolyn L. Ren

This poster presents a droplet-based microfluidic device integrated with a microwave wave sensor, which is also a heater, for simultaneous sensing and heating of individual nanoliter-sized droplets.

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Rapid Detection of Somatic Mutations in Cancer Genes
Michael J. Powell et. al.,

Rapid higly sensitive detection of tumor gene mutations in DNA derived from FFPE or plasma samples can be achieved with QClamp PCR technology.

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Accurate Normalization of Grain Samples Using Pressure-Based Volume Measurement Technology
Bill Gigante, John Thomas Bradshaw, Tanya Knaide

A common challenge experienced by sample screening facilities is identifying individual sample quantity prior to DNA extraction. Accurately identifying sample quantity allows the user to pipette exact amounts of extraction buffer into each well.

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DHPLC Technology as a High-throughput Detection of Mutations in a Durum Wheat TILLING Population
Colasuonno P.1, Incerti O. 1, Lozito M.L. 1, Sbalzarini M. 2, Zaccagna P. 2, Papadimitriou S. 2, Blanco A. 1, Gadaleta A. 1

This study is a beautiful example of DHPLC technology application and shows an alternative tool to current strategies of SNP detection based on genotyping array.

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IDENTIFICATION AND DIFERENTIATION OF Verticillium SPECIES WITH PCR MARKERS AND SEQUENCING OF ITS REGION
Taja Jesenicnik, Nataša Štajner, Jernej Jakše, Sebastijan Radišek and Branka Javornik

The genus Verticillium is a group of ascomycete fungi, including plant-pathogenic species capable of affecting the vasculature of many agricultural crops, and therefore causes major economic losses worldwide. In 2011, a new taxonomic classification of the genus was proposed, which is now referred to as Verticillium sensu stricto, comprising ten species: V. dahliae V. albo-atru, V. alfalfae, V. longisporum, V. nonalfalfae, V. tricorpus, V. zaregamsianum, V. nubilum, V. isaacii and V. klebahnii. <

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Expression Profiling of Circulating Tumor Cells: a Prognostic and Predictive Biomarker in Cancer.
Mikael Kubista, Robert Sjöback,Marie Jindrichova, Eva Rohlova, Vendula Novosadová, Siegfrid Hauch, Bahriye Aktas, Mitra Tewes,Maren Bredemeier, and SabineKasimir-Bauer

We show non-responders to treatment among breast cancer patients can be identified by expression profiling of circulating tumor cells

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Detection in Environmental Samples of Enteroagregative Escherichia coli, stx-producing Escherichia coli and stx-converting Bacteriophage by RT- PCR: Preliminary Data
Mura Elia, Noli Alessia Caterina, Rossi Maria Lucia, Terrosu Giovanni, Fadda Antonio

The aim of this work was to collect epidemiological information concerning the presence of Enteroaggregative Escherichia coli, stx-producing Escherichia coli and stx2a-converting bacteriophage, in ruminants faeces.

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Effect of exposure to stress conditions on propidium monoazide (PMA) - qPCR based enumeration of Campylobacter in broiler carcass rinses
A. Duarte1,2*, N. Botteldoorn2 ,W. Coucke2, S. Denayer2, K. Dierick2, and M. Uyttendaele1

Evaluation and comparison of a developed PMA-qPCR Campylobacter enumeration method with the reference method, after exposure of the broiler rinse artificially contaminated with the bacteria to stress conditions.

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Scientific News
RNAi Screening Trends
Understand current trends and learn which application areas are expected to gain in popularity over the next few years.
The Genetic Roots of Adolescent Scoliosis
Scientists at the RIKEN Center for Integrative Medical Sciences in collaboration with Keio University in Japan have discovered a gene that is linked to susceptibility of Scoliosis.
Diagnostic Test Developed for Enterovirus D68
researchers at Washington University School of Medicine in St. Louis have developed a diagnostic test to quickly detect enterovirus D68 (EV-D68), a respiratory virus that caused unusually severe illness in children last year.
Simple Technology Makes CRISPR Gene Editing Cheaper
University of California, Berkeley, researchers have discovered a much cheaper and easier way to target a hot new gene editing tool, CRISPR-Cas9, to cut or label DNA.
HPV Genomes Show Greater Diversity Than Expected in Cancer Patients
The findings could have implications for eventually understanding why some cervical lesions become malignant.
Rapidly Detecting Drug-Resistant HepC
A nested PCR-based assay has been shown to rapidly and accurately detect drug-resistant strains of the hepatitis C virus.
Researchers Seek Water Test for Invasive Species Detection
Detecting invasive lake and river species using just a water sample would be a dream come true for wildlife managers and regulators in the state and University of Maine researchers may soon make this an inexpensive reality.
New Cell Structure Finding Might Lead to Novel Cancer Therapies
University of Warwick scientists in the U.K. say they have discovered a cell structure which could help researchers understand why some cancers develop.
Ebola Assays Compared in Head-to-Head Analysis
A newly published study has attempted to rigorously evaluate a few of the assays recently granted Emergency Use Authorization by the US Food and Drug Administration to test for Ebola Zaire virus.
Profiling DNA Viruses in Arctic Lakes
The Arctic's freshwater lakes contain viral communities composed of DNA viruses from lineages that are largely distinct from those described elsewhere, a new study suggests.
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