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Detection in Environmental Samples of Enteroagregative Escherichia coli, stx-producing Escherichia coli and stx-converting Bacteriophage by RT- PCR: Preliminary Data
Mura Elia, Noli Alessia Caterina, Rossi Maria Lucia, Terrosu Giovanni, Fadda Antonio

The aim of this work was to collect epidemiological information concerning the presence of Enteroaggregative Escherichia coli, stx-producing Escherichia coli and stx2a-converting bacteriophage, in ruminants faeces.

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Effect of exposure to stress conditions on propidium monoazide (PMA) - qPCR based enumeration of Campylobacter in broiler carcass rinses
A. Duarte1,2*, N. Botteldoorn2 ,W. Coucke2, S. Denayer2, K. Dierick2, and M. Uyttendaele1

Evaluation and comparison of a developed PMA-qPCR Campylobacter enumeration method with the reference method, after exposure of the broiler rinse artificially contaminated with the bacteria to stress conditions.

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Quantitative Real-time PCR differentiation between Genetically Modified pollen and Genetically Modified flour in honey
Eugénia de Andrade1*, Maria Lopes2, Maria Clara Fernandes1, Fátima Quedas2, Isabel Rodrigues1, Amélia Maria Lopes1


We investigated the ability of quantitative real-time PCR, using plasmids calibrants, together with the triploid maize seed endosperm and the haploid pollen, to get good estimates for the copy number of a transgene in relation to the copy number of a species specific gene as a mean to distinguish between pollen and flour from maize.

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Digital PCR to Determine the Number of Transcripts from Single Neurons after Patch-clamp Recording
Nóra Faragó1,2, Ágnes K. Kocsis3, Sándor Lovas3, Gábor Molnár3, Márton Rózsa3, Viktor Szemenyei3, Ágnes Zvara2, Gábor Tamás3, László G Puskás1,2

Whole-cell patch-clamp recording enables detecting electrophysiological signals from neurons, and RNA can be harvested into the patch pipette from the cells.We have optimized a dPCR protocol for determining exact transcript numbers in single neurons after patch-clamp recording by using dPCR based on high-density nanocapillary PCR.

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On-chip quantification of miRNA using digital droplet PCR
Q. Cai1, R.S. Wiederkehr1, B. Jones1, B. Majeed1, T. Stakenborg1, P. Fiorini1, L. Lagae1, M Tsukuda2, T. Matsuno2, I. Yamashita2

miRNAs have a great potential in diagnostics. Hence, automated profiling of miRNAs are of great interest. In-house technology show that it is possible to implement a multiplexing assay for miRNAs on a microfluidic chip using digital droplet PCR.

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A multianalyte algorithm PCR-based blood test outperforms single analyte ELISA-based blood tests for neuroendocrine tumor detection
Mark Kidd, Irvin M Modlin, Daniele Alaimo, Stephen Callahan, Nancy Teixiera, Lisa Bodei, Ignat Drozdov

In a prospective study, in age-/sex- and ethnicity-matched patients and controls (n=82), a 51 panel multigene blood transcript test (NETest) was identified to be significantly more sensitive and accurate (>93%) than any single analyte assay (Chromogranin A, Pancreastatin or Neurokinin A) for neuroendocrine tumor detection.

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Stealth-Adapted Viruses and Viteria: Insights into Virus Construction, Replication and Potential Therapies
W. John Martin

There is an increasing incidence of diseases with accompanying signs and symptoms of brain damage. These include neurological and psychiatric illnesses, childhood behavioral disorders, and such common conditions as chronic fatigue, Gulf War Syndrome, so-called “chronic Lyme disease”, and many cancers. Altogether, these diseases have an enormous social impact.

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High-Throughput Analysis of DNA Samples using the D1K ScreenTape Assay and the Agilent 2200 TapeStation System
Arunkumar Padmanaban, Ruediger Salowsky, Adam Inche

Recent advances in genomics demands to look at a wealth of genetic information in a short period of time. DNA analysis using slab gel electrophoresis and capillary electrophoresis are widely being used as a QC step in next generation sequencing and microarray studies. However, often these techniques lack the speed and involve more manual steps to perform the assay.

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Hot Start dNTPs – Pushing the Limits of PCR
Tony Le, Hidalgo Ashrafi, Sabrina Shore, Victor Timoshchuk, Natasha Paul, Richard Hogrefe, Inna Koukhareva, Alexandre Lebedev

Hot Start dNTPs are a distinct approach that employs modified nucleoside triphosphates with a thermolabile protecting group. This modification blocks low temperature primer extension and is released at higher temperatures to allow for more specific DNA polymerase incorporation.

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Scientific News
Top 10 Life Science Innovations of 2016
2016 has seen the release of some truly innovative products. To help you digest these developments, The Scientist have listed their top picks for the year.
Using Cancer Cells' Mass to Predict Treatment Response
A device has been developed that can detect changes in cell mass at a minute scale.
NVIDIA Awards $400k to Trailblazers in Cancer Research
NVIDIA Foundation furthers research that could lead to new and more targeted treatments with investments.
Malaria Parasite Evades Rapid Test Detection in Children
A study at the University of North Carolina found that gene deletion poses a threat to Malaria eradication efforts.
Working With Advanced Nanoimagers
Oxford Nanoimaging report on the work of early-adopters for their Nanoimager technology at the MRC Centre for Molecular Bacteriology and Infection.
History of Cells Told Through MEMOIR
MEMOIR technique developed by CalTech researchers enables the life history of cells to be read.
Nanoscale ‘Muscles’ Powered by DNA
Scientists have developed nanoscale "muscles" to integrate with custom DNA, that can force the material to bend, curl and flip.
Dissecting Bacterial Infections at the Single-Cell Level
Researchers have used single-cell analysis technology to provide new insight into the Salmonella infection process.
Peer Review is in Crisis, But Should be Fixed, Not Abolished
After the time to get the science done, peer review has become the slowest step in the process of sharing studies, and some scientists have had enough.
Cancer Stem Cells Fuel Tumor Growth
Mass. General, Broad Institute team finds strong evidence that cancer stem cells are important drivers of tumors in patients.
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