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Tuesday, September 02, 2014
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Novel, Fully Automated Method Allows Efficient Analysis of qPCR Data for Qualitative Calling Based on Comparative Cq
Collaborative research by Pioneer Hi-Bred (a DuPont company) and Azure PCR Limited

Assessment to ascertain if a method for analysis of qPCR data dependent on manual intervention can be replaced by automated analysis using the AzurePCRTM method.

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Volume-Related Inhibitors Standardization for Reverse Transcription Quantitative Polymerase Chain Reaction Experiments
Pascal Pugniere, Sebastien Banzet, Thomas Chaillou, Catherine Mouret and Endre Peinnequin

This poster addresses the reliability of qPCR data and its dependence on technical variations. The proposal is that constant volume of RNA extract can improve reliability of RT-qPCR.

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Defining off-target cleavage in a pair of Zinc Finger Nucleases
K. Mukherjee, D. Carroll

This study looks at off-target cleavage of Zinc Finger Nucleases (ZNFs) in Drosophila in an attempt to analyze potential cleavage spots, with a view to designing more efficient ZFNs.

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Gene Expression Profiling: qPCR Toolkit for Quality Control
Švec D., Jacobsson J., Sjöback R., Kubista M.

TATAA Biocenter explain how they developed and optimized high-throughput gene expression qPCR with ValidPrime quality control, compensating for inter-run variations.

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Rapid PCR for Integration in Sample-to-answer Analysis Platforms
S. Brunklaus, T.E. Hansen-Hagge, J. Erwes, J. Höth, M. Jung, D. Latta, X. Strobach, C. Winkler, T. Röser, M. Ritzi-Lehnert, K.S. Drese

This poster describes how molecular testing at the point-of-care can increase time to results and yield rather specific information, concentrating on PCR on a chip layout which proves to be fast and robust.

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Hot Start Amplification using OligoBeads via Gradual Release of Bound Primers
Dr. Nam Ngo, Dr. Laurent Jacquinod

OligoBeads provide a mean to store normalized primers used in performing enzymatic reactions including PCR. Primer bound beads eliminate the potential for pipeting errors and reduce contamination thus yielding lower repeat rates and less reagent wastage. The primers bound to the OligoBeads can be stored over a period of a few months without degradation in a nuclease free environment.

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Target length effect on sensitivity and specificity of oligonucleotide microarrays: Advantages of a modified PCR based labelling method over the dendrimer technology.
Abdullah Gibriel1*, Walter Kolch2 and Andrew Pitt1,3

Several methods have been developed for target labelling to enable DNA microarray quantification without taking careful consideration for target length effect. This report highlights the importance of choosing the optimum target length that would ensure specificity without compromising sensitivity of the assay. It also shows the advantages of using the modified PCR method over other methods in generating labelled amplicons of the desired lengths to maximize hybridization efficiency.

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On chip micro-extraction and real-time PCR with integrated SPAD optical fluorescence detection for nucleic acid analysis
Cristina Potrich, Elisa Morganti, Nicola Massari, Lucio Pancher, C. Kostoulas, Laura Pasquardini, Cristian Collini, Andrea Adami, Lorenzo Lunelli, F. Kalatzis, David Stoppa, Cecilia Pederzolli, Leandro Lorenzelli

A PDMS lab-on-a-chip for one step DNA isolation and real time-polymerase chain reaction (RT-PCR) has been designed, fabricated, and characterized for point-of-care clinical diagnostics. In addition, a module for on-chip optical detection based on SPAD - Single-Photon Avalanche Diode - detector has also been developed and used to monitor the presence of specific DNA polymorphisms possibly related to genetic diseases.

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Chemical Variant of 7-deaza-dGTP for Improved CG-rich PCR Amplification
E. H. Ashrafi, S. Shore, T. Le, V. Timoshchuk, N. Paul, R. Hogrefe, G. Zon, I. Koukhareva, A. Lebedev

PCR amplification of nucleic acids is a fundamental technique used in many molecular biology laboratories. Despite its wide use, certain GC-rich regions of DNA, such as mycobacterial disease targets, still remain a challenge for amplification. Sequences high in GC content are associated with the formation of secondary structure, which prevents adequate strand separation and DNA polymerase amplification. As a consequence, mispriming is prominent, complicating specific product formation.

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Showing Results 11 - 20 of 84
Scientific News
Detecting and Identifying Candida Species in Blood Samples of Critically Ill Paediatric Patients
The study aimed to develop a multiplex nested PCR method to detect and identify seven Candida species in peripheral blood samples of critically ill paediatric patients.
IDT Shares Comprehensive qPCR Resources
Special qPCR compendium issue of DECODED now freely available.
Determination of Arabidopsis Thaliana Telomere Length by PCR
Researchers have designed two telomeric degenerated primers that amplify Arabidopsis telomeres by MMQPCR.
Methods for Multiplex Template Sampling in Digital PCR Assays
This article presents Multiplex Template Sampling, an alternative strategy to effectively increase template concentrations in dPCR analysis.
Detection of Large Expansions in Myotonic Dystrophy Type 1
The aims of this study were to verify the validity of triplet primed-PCR for the diagnosis of patients presenting with DM1 clinical findings and to simplify the testing procedure.
The 2014 Annual MO BIO Microbiome Awards, Offering More Than $10,000 of Prizes
The Microbiome Awards aim to provide young, extraordinary scientists with funding and recognition to carry out scientific work in the field of microbiome research.
Genetic Discovery Could Aid Diagnosis of Childhood TB
A distinctive genetic 'signature' found in the blood of children with TB offers new hope for improved diagnosis of the disease.
Genotoxicity Evaluation of Acephate and Profenofos by the PCR-RFLP Assay
The rampant use of organophosphorus insecticides has created a chemical environment which is proving harmful to the living system.
Strong Link Between Obesity and 'Carb Breakdown' Gene
Findings suggest that dietary advice may need to be tailored to individual's digestive system.
An Alternative Suite of Universal Primers for Genotyping in Multiplex PCR
The new set of universal primers for multiplex genotyping were proven to be efficient and reliable in varied species.
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