|A Web Interface for Automatic Microarray Analysis|
Enrico Glaab, Jonathan M. Garibaldi, Natalio Krasnogor
DNA-Microarray technology provides a diagnostic tool for studying cancer and genetic diseases, allowing the experimenter to identify disease related genes and predict the tumour type for new cell samples. However, the statistical analysis of microarray data is a time-consuming and error-prone process. To address this problem we have developed a web interface for automatic DNA-microarray analysis, ArrayMining.net, which guides the user through the analysis process and performs automatic parameter
|A modular and stochastic approach to the study of gene circuits using P systems|
Romero-Campero FJ, Blakes J, Cao H, Camara M, Krasnogor N.
presented at Genomes to Systems Manchester, UK March 17-19 2008
|Formal informatics and machine learning for more principled systems and synthetic biology|
Romero-Campero FJ, Blakes J, Camara M, Willams P, Perez-Jimenez MJ, Krasnogor N
presented at European Conference on Synthetic Biology (ESF-UB Conference in Biomedicine), Sant Feliu de Guixols, Spain, 24-29 November 2007.
|A systems analysis of the AHL Quorum Sensing system in Pseudomonas aeruginosa|
Romero-Campero FJ, Blakes J, Camara M, Krasnogor N.
presented at Systems Biology (ESF-UB Conference in Biomedicine), Sant Feliu de Guixols, Spain, 12-17 April 2008
|An Integrated Development Environment for Synthetic Biology Models|
Blakes J, Romero-Campero FJ, Twycross J, Cao H, Krasnogor N
presented at European Conference on Synthetic Biology (ECSB) II: Design, Programming and Optimisation of Biological Systems (ESF-UB Conference in Biomedicine), Sant Feliu de Guixols, Spain, 29 March - 03 April 2009
|Potential Use of RNAi on Human Skin|
Christine Collin-Djangoné, Florent Sahuc, Béatrice Bertino, Anne de Brugerolle, Jean-Eric Baudouin, Peggy Sextius, Séverine Teluob, Rachid Boulgana, Dmitry Samarsky, Peggy Tailor, Peter Welch and Yann Mahé
We designed highly efficient StealthTM RNAi targeting human tyrosinase with optimum IC50 in the 10-100 pM range. By silencing tyrosinase in melanocytes, we achieved the reconstruction of an artificial epidermis showing a long term lasting (>30 days) inhibition of melanogenesis. Since skin pigmentation can phenotypically be monitored, the use of StealthTM RNAi against tyrosinase on reconstructed pigmented epidermis is a precious tool for the evaluation of delivery vehicles.
|A Novel Multiplexed Digital Gene Expression Technology|
Gary K. Geiss1,#, Roger Bumgarner2, Brian Birditt1, Timothy Dahl1, Naeem Dowidar1, Dwayne L. Dunaway1, H. Perry Fell1, Sean Ferree1, Renee D. George1, Tammy Grogan1, Jeffrey J. James1, Malini Maysuria1, Jeffrey D. Mitton1, Paola Oliveri4, Jennifer L. Osborn3, Tao Peng2, Amber L. Ratcliffe1, Philippa J. Webster1, Eric H. Davidson4, and Leroy Hood5
We describe a novel platform, the nCounter Analysis System, for sensitive, highly multiplexed, digital gene expression analysis based on NanoString’s novel molecular barcoding technology. Detection of individual mRNA molecules using an assigned sequence of six different fluorescent spots per probe are detected, and then the number of times that code sequence appears in a sample are counted.
|Biosynthesis of Very Long Chain Polyunsaturated Fatty Acids in Chicory|
Hattem Mekky1, Michael R. Davey1, J. Brian Power1 , Maged E. Mohamed2 and Colin M. Lazarus2
Very long chain polyunsaturated fatty acids (VLCPUFAs) are declining in reserves, since they are produced by oily fish. VLCPUFAs are important in human nutrition because they are responsible for the production of inflammatory mediators such as prostaglandins, the regulation of cholesterol synthesis and its transport, and the maintenance of cellular membranes. This research aims to produce VLCPUFAs in edible plants.
|Oligonucleotides with LNA and targeting of biologically important RNAs|
Peter Guterstam and Ülo Langel
Activity of RNA-targeting antisense oligonucleotides increases when introducing LNA monomers, implying that an 18 nucleotides long LNA/2OMe mixmer splice-switching oligonucleotide can be shortened to 12mer and have similar activity and specificity as an 18mer 2OMe oligonucleoyide. Positioning of LNA monomers has to be carefully considered when utilizing the potent LNA monomers in RNA-targeting antisense oligonucleotides.