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Hot Start dNTPs - A Novel Tool for Controlled Nucleotide Incorporation in PCR
Tony Le, Elena Hidalgo Ashrafi, Sabrina Shore, Victor Timoshchuk, Natasha Paul, Richard Hogrefe, Inna Koukhareva, Alexandre Lebedev

PCR is a widely used scientific tool employed by a variety of applications. Various Hot Start technologies have already been developed using modified PCR components to increase specificity of a reaction. Recently developed CleanAmpTM dNTPs are modified nucleoside triphosphates with a thermolabile 3’-tetrahydrofuranyl protecting group that is released at higher temperatures. These modified dNTPs prevent low temperature primer extension, which can often be a significant problem in PCR. At higher t

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Evaluation of microfluidic digital PCR for the detection of cancer biomarkers
Rebecca Sanders, Claire Bushell, Carole Foy, Daniel J. Scott

dPCR is achieved by sample partitioning prior to PCR amplification such that each reaction chamber contains one copy or less of target DNA. This dilution becomes the limiting factor and an accurate target molecule count is achievable. This study evaluates dPCR’s quantitative capabilities and investigates parameters influencing copy number quantification, using the Fluidigm Biomark instrument. Biomark technology combines dPCR theory with a microfluidics platform.

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Inhibition of DNA methylation does not overcome docetaxel resistance in human breast cancer cells
Kastl L, Brown I, Schofield A

DNA mehtylation can lead to chemotherapy resistance in cancer. The aim of this study was to investigate the role of DNA methylation in docetaxel-resistant human breast cancer cells. Whereas the DNA methylation machinery is altered in docetaxel-resistant human breast cancer cells compared to docetaxel-sensitive human breast cancer cells, resistance to docetaxel could not be reversed using the DNA methylation inhibitor decitabine.

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A novel multiplex qPCR for the simultaneous detection of Salmonella spp., E. coli O157:H7 and Listeria monocytogenes from raw Atlantic Salmon fillets
Sonja S. Nitecki, Brian F. Carney, John W. Slater, Wolfram M. Bruck

A triplex qPCR assay was developed to screen for three major food-borne pathogens in fish. The assay is based on simultaneous qPCR amplification of specific target genomes using fluorophore labelled locked nucleic acid (LNA) TaqMan probes. The detection limit of the qPCR is 1 colony forming unit (cfu) per 1ml and the detection limit of the assay with 24h general enrichment is 1 cfu per 25g of fish meat.

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Insulin Decreases Transcription of Three Proteins Associated with Lung Surfactant
Rucka, Z [a], Vanhara, P [b], Tesarova, L [a], Potesilova, M [a], Stejskal, S [a], Dolezel, J [c], Koutna, I [a]

Our project is partially focused on insulin affecting four proteins associated with lung surfactant. Small and hydrophobic SP-B and S-PC help to spread and stabilize the surfactant layer while large and hydrophilic SP-A and SP-D participate in immune responses. A549 and H441 cell lines were used. It was observed using RealTime PCR that higher doses of insulin lead to decreased transcription of SP-B, SP-C, SP-D, and both isoforms of SP-A.

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Tapping into the mechanical links of expansins in leaf morphogenesis
Hoe-Han, Goh

Expansins are conserved family of non-enzymatic proteins involved in cell wall-loosening accounted for widespread role in plant development. My research focuses on functional analysis of expansins during leaf morphogenesis. Atomic force microscopy was applied to characterise cell wall mechanical properties in an attempt for in vivo quantification of expansin activity. It’s showed how that there is a distinct difference in cell wall extensibility between leaf abaxial and adaxial side.

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SYBR® Green I as a biomarker for schizophrenia
Davood Zaeifi, Mehdi Rahmati, Vahab Piranfar

The purpose of this study were to examine if the mRNA of the peripheral dopamine receptor is changed in schizophrenia patients. 50 Naive patients were enrolled. After extracting RNA from white blood cells, cDNA is synthesized. After doing the quantitative Real-time PCR, expression of D3 receptors in healthy persons and patients were compared. Results of this examinations reveals that expression of D3 receptor is increased in compared with controls sample.

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Evaluation of a Novel Approach for the Measurement of RNA Quality
Timothy Wilkes; Alison Devonshire; Carole Foy

The potential medical applications of microarrays have generated much interest, within the biomedical community. Numerous potential sources of variation have raised concerns regarding assay consistency and data quality. Previous studies have highlighted RNA integrity as having a major effect on data quality. We describe here a comparison of the performance characteristics of the Agilent (Bioanalyser) and Lab901 (Screen Tape System) platforms, and their capacity to determine sample RNA integrity.

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Microfluidic PCR device for diagnostic pathogen detection
Johannes R. Peham, Hannes Steiner, Walter Grienauer, Rudolf Heer, Michael J. Vellekoop, Christa Nöhammer, Herbert Wiesinger

In this work a microfluidic cyclic flow PCR device is presented, which is capable of replicating the bacterial genomic DNA sequence of the 16S ribosomal RNA. The standard laboratory processing time of 3 h could be decreased to 60 min with the microfluidic reactor without loosing PCR efficiency. Integrating an optical fluorescence detector for dsDNA measurement would evolve this device into a micro total analysis system.

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Showing Results 41 - 50 of 92
Scientific News
New Genomic Research Amends Earlier Triple Negative Breast Cancer Finding
Previously reported molecular finding unable to be validated.
LifeCodexx Reports Successful Proof-of-Principle of Innovative PCR-based NIPT Assay
Breakthrough potential as affordable NIPT solution on global basis.
Blood Test Trumps Accuracy of Standard Screening in Detecting Down Syndrome in Early Pregnancy
A blood test undertaken between 10 to 14 weeks of pregnancy may be more effective in diagnosing Down syndrome and two other less common chromosomal abnormalities than standard non-invasive screening techniques.
Highly Sensitive Detection Of Malaria Parasites
New assays can detect malaria parasites in human blood at very low levels and might be helpful in the campaign to eradicate malaria, reports a study published in PLOS Medicine.
Selection of Reference Genes for Quantitative RT-PCR Analysis
The expression stability of 12 commonly used reference genes was analysed.
‘Liquid Biopsies’ Could Help Spot Genetic Faults in Lung Cancer
Study analyze blood samples from 97 patients who took part in the EURTAC clinical trial.
Accurate Determination of Copy Number States for Multiallelic Copy Number Variations
Researchers use next-generation sequencing (NGS) and Bio-Rad’s Droplet Digital PCR (ddPCR™) technology to solve the technical challenge.
More DNA & Extra Copies of Disease Gene in Alzheimer’s Brain Cells
Scientists at The Scripps Research Institute (TSRI) have found diverse genomic changes in single neurons from the brains of Alzheimer’s patients, pointing to an unexpected factor that may underpin the most common form of the disease.
Single-Cell, 42-plexed Protein Analysis Achieved with a New Microchip Technology
A novel microdevice capable of detecting 42 unique immune effector proteins has been developed.
Quickly Tracing The Source Of Tainted Food
Technology can trace contaminated food to its source.
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