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A Novel Multiplexed Digital Gene Expression Technology
Gary K. Geiss1,#, Roger Bumgarner2, Brian Birditt1, Timothy Dahl1, Naeem Dowidar1, Dwayne L. Dunaway1, H. Perry Fell1, Sean Ferree1, Renee D. George1, Tammy Grogan1, Jeffrey J. James1, Malini Maysuria1, Jeffrey D. Mitton1, Paola Oliveri4, Jennifer L. Osborn3, Tao Peng2, Amber L. Ratcliffe1, Philippa J. Webster1, Eric H. Davidson4, and Leroy Hood5

We describe a novel platform, the nCounter Analysis System, for sensitive, highly multiplexed, digital gene expression analysis based on NanoString’s novel molecular barcoding technology. Detection of individual mRNA molecules using an assigned sequence of six different fluorescent spots per probe are detected, and then the number of times that code sequence appears in a sample are counted.

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Biosynthesis of Very Long Chain Polyunsaturated Fatty Acids in Chicory
Hattem Mekky1, Michael R. Davey1, J. Brian Power1 , Maged E. Mohamed2 and Colin M. Lazarus2

Very long chain polyunsaturated fatty acids (VLCPUFAs) are declining in reserves, since they are produced by oily fish. VLCPUFAs are important in human nutrition because they are responsible for the production of inflammatory mediators such as prostaglandins, the regulation of cholesterol synthesis and its transport, and the maintenance of cellular membranes. This research aims to produce VLCPUFAs in edible plants.

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Oligonucleotides with LNA and targeting of biologically important RNAs
Peter Guterstam and Ülo Langel

Activity of RNA-targeting antisense oligonucleotides increases when introducing LNA monomers, implying that an 18 nucleotides long LNA/2OMe mixmer splice-switching oligonucleotide can be shortened to 12mer and have similar activity and specificity as an 18mer 2OMe oligonucleoyide. Positioning of LNA monomers has to be carefully considered when utilizing the potent LNA monomers in RNA-targeting antisense oligonucleotides.

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miRNAs in Treating Cardiomyopathy
Veenu Aishwarya

The study aims to design antigomirs against miRNAs involved in Cardiomyopathy. Potential miRNAs involved in the down regulation of certain important genes during this disorder have been identified. All reported miRNAs were scanned using an algorithm against these genes. At three step protocol was followed to take care of false positives and false negatives. Further, HL-1 cells (cardiomyocytes) are been transfected by anti-miRNAs for confirmation.

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Comparison of two different real time PCR assays and chemistry for detection of norovirus genogroup II. Method of choice?
Toplak Nataša, Zimšek Mijovski Janet, Kovac Minka, Poljšak-Prijatelj Mateja

Noroviruses (Caliciviridae) are one of the main cause of acute non-bacterial gastroenteritis in humans. Noroviruses can not be propagated in cell cultures. Thus Real Time PCR molecular methods for caliciviral detection have been recently introduced. The aim of the study was to compare two different Real Time PCR assays for detection of norovirus strains (genogroup II) and the comparison between different RT-PCR reagents and Real Time PCR instruments was made.

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Comparison of two different real time PCR assays and chemistry for detection of norovirus genogroup II. Method of choice?
Toplak Nataša, Zimšek Mijovski Janet, Kovac Minka, Poljšak-Prijatelj Mateja

Noroviruses (Caliciviridae) are one of the main cause of acute non-bacterial gastroenteritis in humans. Noroviruses can not be propagated in cell cultures. Thus Real Time PCR molecular methods for caliciviral detection have been recently introduced. The aim of the study was to compare two different Real Time PCR assays for detection of norovirus strains (genogroup II) and the comparison between different RT-PCR reagents and Real Time PCR instruments was made.

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qPCRas a primary screen in drug discovery
GauravJaggi, Frank Boeckler, Andreas Joerger and Alan Fersht

We report the use of qPCR technique to follow the thermal unfolding of proteins by the binding of the dye SYPRO Orange, and exploit its potential as a robust and high-throughput primary screen for small molecule drug discovery.

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Targeting Inflammatory Cytokines Using Adenoviruses: gene delivery of biological therapies in ovarian cancer
Michael A. Salako, Hagen Kulbe, Iain A. McNeish, Frances R. Balkwill

Constitutive TNF-alpha expression is characteristic of the malignant ovarian surface epithelium. Adenoviral mutants hold great promise as gene therapy vectors but their efficacy is hindered by an inflammatory cascade orchestrated by TNF-alpha. We found that delivering TNF-alpha shRNA to ovarian cancer cells using oncolytic adenoviruses could reduce the inflammatory cascade generated by adenoviruses and also had direct anti-tumour activity on the cancer cells.

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Efficient downregulation of the lung liquid clearing gammaENaC subunit by RNAi
Nihal Yueksekdag, Marei Drechsel, Christa Schmidt, and Josef Rosenecker

CF is caused by mutations in the gene encoding for CFTR. CFTR functions as chloride channel on the apical membrane of epithelia thereby regulating the transport of chloride and also sodium ions indirectly. It seems that the regulation of ENaC fails due to the mutated CFTR protein. And it is assumed that ENaC plays a role in the pathogenesis of chronic lung disease in CF-patients.

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Scientific News
The Genetic Roots of Adolescent Scoliosis
Scientists at the RIKEN Center for Integrative Medical Sciences in collaboration with Keio University in Japan have discovered a gene that is linked to susceptibility of Scoliosis.
Diagnostic Test Developed for Enterovirus D68
researchers at Washington University School of Medicine in St. Louis have developed a diagnostic test to quickly detect enterovirus D68 (EV-D68), a respiratory virus that caused unusually severe illness in children last year.
Simple Technology Makes CRISPR Gene Editing Cheaper
University of California, Berkeley, researchers have discovered a much cheaper and easier way to target a hot new gene editing tool, CRISPR-Cas9, to cut or label DNA.
RNAi Screening Trends
Understand current trends and learn which application areas are expected to gain in popularity over the next few years.
HPV Genomes Show Greater Diversity Than Expected in Cancer Patients
The findings could have implications for eventually understanding why some cervical lesions become malignant.
Rapidly Detecting Drug-Resistant HepC
A nested PCR-based assay has been shown to rapidly and accurately detect drug-resistant strains of the hepatitis C virus.
Researchers Seek Water Test for Invasive Species Detection
Detecting invasive lake and river species using just a water sample would be a dream come true for wildlife managers and regulators in the state and University of Maine researchers may soon make this an inexpensive reality.
New Cell Structure Finding Might Lead to Novel Cancer Therapies
University of Warwick scientists in the U.K. say they have discovered a cell structure which could help researchers understand why some cancers develop.
Ebola Assays Compared in Head-to-Head Analysis
A newly published study has attempted to rigorously evaluate a few of the assays recently granted Emergency Use Authorization by the US Food and Drug Administration to test for Ebola Zaire virus.
Profiling DNA Viruses in Arctic Lakes
The Arctic's freshwater lakes contain viral communities composed of DNA viruses from lineages that are largely distinct from those described elsewhere, a new study suggests.

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