Corporate Banner
Satellite Banner
qPCR
Scientific Community
 
Become a Member | Sign in
Home>Resources>Posters

  Posters

A novel multiplex qPCR for the simultaneous detection of Salmonella spp., E. coli O157:H7 and Listeria monocytogenes from raw Atlantic Salmon fillets
Sonja S. Nitecki, Brian F. Carney, John W. Slater, Wolfram M. Bruck

A triplex qPCR assay was developed to screen for three major food-borne pathogens in fish. The assay is based on simultaneous qPCR amplification of specific target genomes using fluorophore labelled locked nucleic acid (LNA) TaqMan probes. The detection limit of the qPCR is 1 colony forming unit (cfu) per 1ml and the detection limit of the assay with 24h general enrichment is 1 cfu per 25g of fish meat.

More information
Insulin Decreases Transcription of Three Proteins Associated with Lung Surfactant
Rucka, Z [a], Vanhara, P [b], Tesarova, L [a], Potesilova, M [a], Stejskal, S [a], Dolezel, J [c], Koutna, I [a]

Our project is partially focused on insulin affecting four proteins associated with lung surfactant. Small and hydrophobic SP-B and S-PC help to spread and stabilize the surfactant layer while large and hydrophilic SP-A and SP-D participate in immune responses. A549 and H441 cell lines were used. It was observed using RealTime PCR that higher doses of insulin lead to decreased transcription of SP-B, SP-C, SP-D, and both isoforms of SP-A.

More information
Tapping into the mechanical links of expansins in leaf morphogenesis
Hoe-Han, Goh

Expansins are conserved family of non-enzymatic proteins involved in cell wall-loosening accounted for widespread role in plant development. My research focuses on functional analysis of expansins during leaf morphogenesis. Atomic force microscopy was applied to characterise cell wall mechanical properties in an attempt for in vivo quantification of expansin activity. It’s showed how that there is a distinct difference in cell wall extensibility between leaf abaxial and adaxial side.

More information
SYBR® Green I as a biomarker for schizophrenia
Davood Zaeifi, Mehdi Rahmati, Vahab Piranfar

The purpose of this study were to examine if the mRNA of the peripheral dopamine receptor is changed in schizophrenia patients. 50 Naive patients were enrolled. After extracting RNA from white blood cells, cDNA is synthesized. After doing the quantitative Real-time PCR, expression of D3 receptors in healthy persons and patients were compared. Results of this examinations reveals that expression of D3 receptor is increased in compared with controls sample.

More information
Evaluation of a Novel Approach for the Measurement of RNA Quality
Timothy Wilkes; Alison Devonshire; Carole Foy

The potential medical applications of microarrays have generated much interest, within the biomedical community. Numerous potential sources of variation have raised concerns regarding assay consistency and data quality. Previous studies have highlighted RNA integrity as having a major effect on data quality. We describe here a comparison of the performance characteristics of the Agilent (Bioanalyser) and Lab901 (Screen Tape System) platforms, and their capacity to determine sample RNA integrity.

More information
Microfluidic PCR device for diagnostic pathogen detection
Johannes R. Peham, Hannes Steiner, Walter Grienauer, Rudolf Heer, Michael J. Vellekoop, Christa Nöhammer, Herbert Wiesinger

In this work a microfluidic cyclic flow PCR device is presented, which is capable of replicating the bacterial genomic DNA sequence of the 16S ribosomal RNA. The standard laboratory processing time of 3 h could be decreased to 60 min with the microfluidic reactor without loosing PCR efficiency. Integrating an optical fluorescence detector for dsDNA measurement would evolve this device into a micro total analysis system.

More information
Differential metabolic response of tomato hybrids to herbivory
Raghava T, Puja Ravikumar, Rajendra Hegde, Anil Kush

In this study we have documented the HIPVs produced by different plant organs in three agronomically important Lycopersicon esculentem (tomato) cultivars in the event of herbivory. The nocturnal and diurnal feeding of third instar Spodoptera littura larvae induces characteristic VOCs mainly terpenes, in all the three hybrids viz., All Rounder, Shaktiman and Lakshmi.

More information
Design and Fabrication of a Micro PCR Module for POC Applications
E. Morganti, C. Collini, C. Ress, A. Adami, L. Lorenzelli

The design and fabrication process of a micro PCR module is presented. The final system will be integrated in an innovative Lab on a Chip (LOC) to provide a technological platform able to detect autoimmune genetic diseases.

More information
A Web Interface for Automatic Microarray Analysis
Enrico Glaab, Jonathan M. Garibaldi, Natalio Krasnogor

DNA-Microarray technology provides a diagnostic tool for studying cancer and genetic diseases, allowing the experimenter to identify disease related genes and predict the tumour type for new cell samples. However, the statistical analysis of microarray data is a time-consuming and error-prone process. To address this problem we have developed a web interface for automatic DNA-microarray analysis, ArrayMining.net, which guides the user through the analysis process and performs automatic parameter

More information
<< 4 5 6 7 8 9 10 >>
Showing Results 61 - 70 of 109
Scientific News
Assessing the Effectiveness of Genome-Editing Technologies
Researchers have developed a cost-effective and rapid method for assessing edits generated by CRISPR-Cas9 and other genome-editing technologies.
Measuring the Abundance of Extremely Rare Mutations
Researchers from Rutgers University demonstrate the use of multiplex real-time PCR assays to measure the abundance of extremely rare mutations associated with cancer.
Harnessing Helpful Microbes
Seeking to further harness microbes’ many uses, the federal government has launched the National Microbiome Initiative (NMI) to “foster the integrated study of microbiomes across different ecosystems.”
What Makes a Good Scientist?
It’s the journey, not just the destination that counts as a scientist when conducting research.
New Tool Could Change How Infectious Diseases Are Diagnosed
Scientists at the University of Utah School of Medicine, ARUP Laboratories, and IDbyDNA, Inc., have developed ultra-fast, meta-genomics analysis software called Taxonomer that dramatically improves the accuracy and speed of pathogen detection.
Benchtop Automation Trends
Gain a better understanding of current interest in and future deployment of benchtop automated systems.
Finding Zika One Paper Disc At A Time
A novel, inexpensive method for detecting the Zika virus could help slow spread of outbreak, and potentially other future pandemic diseases.
Rapid Diagnosis of Bacterial Infections
Mass. General-developed compact system could shorten diagnostic time from days to hours, bring testing to point of care.
Monovar Drills Down Into Cancer Genome
Rice, MD Anderson develop program to ID mutations in single cancer cells.
Biomarkers for Profiling Prostate Cancer Patients
Exiqon A/S has announced the publication of validation of prognostic microRNA biomarkers for the aggressiveness of prostate cancer in independent cohorts.

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!