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Quantification of siRNA by a Novel Competitive-qPCR Method
Wei-li, Liu., Mark Stevenson and Len Seymour

We have developed a competitive qPCR method in which siRNA competes with a homologous forward primer to bind template DNA, giving siRNA concentration dependent inhibition. The addition of E6-siRNA to cqPCR led to inhibition of amplification in a linear concentration-dependent manner, with as little as 200pg of siRNA capable of being detected. Irrelevant siRNA had no effect on amplification confirming specificity

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The Silencing of Multidrug Resistance-Associated Protein 5 by siRNA Complexes
M.Malinen, E.Mannermaa, T.Ryhänen, E.Kuusela, M.Häkli, M.Yliperttula and A.Urtti

The purpose is to study the role of multidrug resistance-associated protein 5, MRP5 in drug metabolism in human retinal pigment epithelium (RPE). RPE forms the outer part of blood-retinal barrier (BRB) which restricts movements of solutes from systemic bloodstream to the neural retina. The efflux protein, MPR5 is expressed in RPE but its functions are mainly unknown. SiRNA will be tested as a tool to clarify the role of MRP5.

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Caveolin-1 Expression as a Possible Biomarker in Pancreatic Cancer Diagnosis
C. Tanase, E. Raducan, L. Albulescu, E. Codorean, M.I. Nicolescu, D.I. Popescu, M.L. Cruceru and A.C. Popa

Caveolin1 (Cav-1) function either as a tumor supressor or as a promoter of metastasis. Overexpresion of cav-1 was correlated with: tumoral grading, proliferration markers (Ki67, p53), serum tumor markers (CEA, CA19.9) and angiogenic markers (VEGF, bFGF).

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New Transcripts Identified for S. cerevisiae, S. pombe and Drosophila Using Novel cDNA Cloning
Les Hoffman, Janina Gornemann, Erica Rodriguez and David Brow

Unannotated transcripts, including antisense, noncoding, intergenic, and potential regulatory RNAs, were identified in three different eukaryotic model organisms by cDNA cloning and sequencing. A rapid double-stranded cDNA generation system will be described.

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Preliminary Report: The Geriatric Propamed Study: Prospective pharmacopgenetics in geriatrics
Dr LS Griffith, Dr L Chialda and Dr A Pahl

In a worldwide first proscpective phamacogenetic study preliminary results show reduction of adverse drug reactions and hospitilisation stays for geriatric patients after pharmacogenetic testing and medication interaction analysis to fit the medicine to the patient.

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Expression Profiling of Receptor-like Cytoplasmic Protein Kinase (class VI) of Arabidopsis
Manuela Elena Jurca and Attila Feher

Gene expression pattern of 14 members of RLCK class VI in Arabidopsis is described. qRT-PCR was used to determine the transcript levels in the various organs of plant as well as under a series of abiotic stress/hormone treatments in seedlings.

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Changes in the Gene Expression of mRNA Transcripts for Insulin like Growth Factor, their receptor and Facilitative Glucose Transporter in IVM Oocytes
S.C. Gupta, Neelam Gupta and Alok Pandey

The cDNA libraries from single oocytes and pre-implantation buffalo embryos from 2 cell stage to blastocyst were established. Relative expression studied with RT-PCR of IGF-I showed an increase at 12h and decline at 24h of IVM oocytes. IGF-IR was expressed at cleavage stages to blastocyts. Glut-I was expressed in IVM oocytes and SCNT embryos at all stages. Gene expression of IGF-I, IGF-IR and Glut-I plays an important role in SCNT embryo production.

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DNA Methylation Analysis – Reliable Cell Characterization in Regenerative Medicine
Uli Hoffmueller, Stephen Rapko, Udo Baron, Georg Wieczorek, Alexander Hellwag, Cornelia Krüger, Stefan Kärst, Leslie Wolfe and Sven Olek

We demonstrate that DNA methylation patterns can serve as characteristic markers to distinguish different cell types. We have identified panels of methylation markers that are specific to mesenchymal stem cells or various differentiated cell types in the mesenchymal lineage. This method of cell type identification has a number of advantages over conventional markers in that it is robust, is both qualitative and quantitative.

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EM Algorithm for Gene Copy Number Estimation Using TaqMan® Assays
Catalin Barbacioru, Kelly Li, Caifu Chen and Raymond Samaha

Recently, TaqMan® assays have been developed for detection of genetic variation at gene level using primers and probes designed for genomic DNA sequences. The R package TaqGCN contains classes and methods that can be used for data reading and plotting, and for predicting gene copy number.

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Scientific News
More DNA & Extra Copies of Disease Gene in Alzheimer’s Brain Cells
Scientists at The Scripps Research Institute (TSRI) have found diverse genomic changes in single neurons from the brains of Alzheimer’s patients, pointing to an unexpected factor that may underpin the most common form of the disease.
Single-Cell, 42-plexed Protein Analysis Achieved with a New Microchip Technology
A novel microdevice capable of detecting 42 unique immune effector proteins has been developed.
Quickly Tracing The Source Of Tainted Food
Technology can trace contaminated food to its source.
Did You Hear The One About The Two Businessmen On A Plane?
EquipNet’s vision ‘to revolutionize the way companies manage their surplus assets’ was first conceived at 30,000 feet somewhere between Chicago and Boston in 1999. Here we learn more about the company's first 15 years.
Developing a Noninvasive Test for Endometriosis
UCSF researchers identify patterns of genetic activity that could help in early detection of disorder.
CDC Develops a New, Faster Lab Test for Enterovirus D68
Confirmed cases will appear to rise as agency accelerates specimen testing.
NanoString, Brigham and Women’s Hospital Collaborate
Research collaboration to accelerate translation of genomic discoveries into clinical diagnostics in oncology.
Unlocking The Non-Coding Half of Human Genome
Texas A&M biologists unlock non-coding half of human genome with novel DNA sequencing technique.
Improving Trout Resistance to a Deadly Disease
A highly sensitive real-time PCR test has been developed to measure F. psychrophilum in fish tissue.
Blood Test To Diagnose Depression
Test identifies nine blood markers tied to depression; predicts who will benefit from therapy.
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