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Wednesday, July 30, 2014
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DNA Methylation Analysis – Reliable Cell Characterization in Regenerative Medicine
Uli Hoffmueller, Stephen Rapko, Udo Baron, Georg Wieczorek, Alexander Hellwag, Cornelia Krüger, Stefan Kärst, Leslie Wolfe and Sven Olek

We demonstrate that DNA methylation patterns can serve as characteristic markers to distinguish different cell types. We have identified panels of methylation markers that are specific to mesenchymal stem cells or various differentiated cell types in the mesenchymal lineage. This method of cell type identification has a number of advantages over conventional markers in that it is robust, is both qualitative and quantitative.

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EM Algorithm for Gene Copy Number Estimation Using TaqMan® Assays
Catalin Barbacioru, Kelly Li, Caifu Chen and Raymond Samaha

Recently, TaqMan® assays have been developed for detection of genetic variation at gene level using primers and probes designed for genomic DNA sequences. The R package TaqGCN contains classes and methods that can be used for data reading and plotting, and for predicting gene copy number.

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EasyBeacons™ - new Probes Ideal for Realtime PCR Detection of Methylation Status of Single CpG Duplets and SNPs
K. Skadhauge, C. Nielsen & U.B. Christensen

The EasyBeacons™ presented here are based on the novel technology Intercalating Nucleic Acid, INA®, linked to a fluorophore and a quencher. INA® is composed of normal DNA nucleotides and Intercalating Pseudo Nucleotides (IPNs). The fact that the EasyBeacons™ are mostly composed of normal DNA nucleotides means that in many respects EasyBeacons™ behave like DNA based probes, allowing use of standard buffers, primers and enzymes and hence reduces the optimisation efforts.

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Real-time PCR Gene Expression Profiling
Mikael Kubista, José Manuel Andrade, Björn Sjögreen and Amin Forootan

Correct interpretation of real-time PCR data requires appropriate experimental design, accurate data pre-processing and analysis of the data using proper statistical and multivariate methods. For this process we have developed the GenEx software.

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Sensitive and Specific Detection of microRNAs
Martin Kreutz, James Qin, Holger Engel, Po-Jen Shih, Peter Hahn, Martin Schlumpberger, Subrahmanyam Yerramilli and Eric Lader

MicroRNAs are endogenous, 21–22 nt, noncoding RNAs that mediate post transcriptional gene regulation. miRNAs are involved in regulation of gene expression during development, differentiation, cell proliferation, and apoptosis. Misregulation of miRNA expression is associated with several cancers and other diseases. We have developed the miScript System for real-time PCR analysis of hundreds of miRNAs, sno RNAs, piRNAs, other small noncoding RNAs.

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Real-time PCR in Diagnostics of Plant Viruses
Mehle N., Boben J. and Ravnikar M.

Sensitive and specific method real-time PCR was developed for diagnostics of certain plant viruses. Real-time PCR can be used for determination of different viruses as well as for differentiation between related strains of viruses. The method can also be used for detection of low concentrations of plant viruses in various samples such as environmental waters, growth substrates and seeds.

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Real-Time Multiplex Rt-PCR on Circulating Tumor Cells
Anieta M. Sieuwerts, Stefan Sleijfer, Jaco Kraan, Joan Bolt-de Vries, Jan-Willem Gratama, John WM Martens and John A. Foekens

Using the CTC kit (CellSearch™), cells that attached to anti-EpCAM Moab were immunomagnetically separated and used for analysis of a selected pilot set of 32 genes by real time RT-PCR. This study shows the feasibility of multiple gene expression analysis on RNA isolated from only one tumor cell. Most importantly, expression analysis of several tumor-specific genes in blood samples containing only 2 tumor cells is already possible.

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Novel Fluidics Microbead Trap/Flow Cell Enhances Speed/Sensitivity of Bead-Based Bioassays Up to 5-Fold
RM Ozanich, CJ Bruckner-Lea, JW Grate, MG Warner, BP Dockendorff, KC Antolick, HC Edberg, LH Johnson, AN Easterday

Pacific Northwest National Laboratory (PNNL) has developed a micro/nano particle trap that allows surface-functionalized magnetic or non-magnetic particles to be trapped with subsequent perfusion of sample, reagents and wash solutions, yielding significant (up to 5-fold) improvements in assay speed and sensitivity, while significantly reducing sample matrix effects.

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Utilizing High Speed Photography to Optimize Low Volume Dispensing Conditions
Mary Cornett, Mitch Gordon and Anca Rothe

In this study we use high-speed photography as a feedback mechanism for adjusting the Nanodrop instrument dispense settings to improve the positional dispense accuracy of low volume (nanoliter) drops. These same parameters can be investigated, with various fluid classes, to reduce deleterious effects on dispensing performance such as deflected streams, satellite formation, secondary pulses and drop deformation.

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Showing Results 71 - 80 of 84
Scientific News
Determination of Arabidopsis Thaliana Telomere Length by PCR
Researchers have designed two telomeric degenerated primers that amplify Arabidopsis telomeres by MMQPCR.
Methods for Multiplex Template Sampling in Digital PCR Assays
This article presents Multiplex Template Sampling, an alternative strategy to effectively increase template concentrations in dPCR analysis.
Detection of Large Expansions in Myotonic Dystrophy Type 1
The aims of this study were to verify the validity of triplet primed-PCR for the diagnosis of patients presenting with DM1 clinical findings and to simplify the testing procedure.
The 2014 Annual MO BIO Microbiome Awards, Offering More Than $10,000 of Prizes
The Microbiome Awards aim to provide young, extraordinary scientists with funding and recognition to carry out scientific work in the field of microbiome research.
Genetic Discovery Could Aid Diagnosis of Childhood TB
A distinctive genetic 'signature' found in the blood of children with TB offers new hope for improved diagnosis of the disease.
Genotoxicity Evaluation of Acephate and Profenofos by the PCR-RFLP Assay
The rampant use of organophosphorus insecticides has created a chemical environment which is proving harmful to the living system.
Strong Link Between Obesity and 'Carb Breakdown' Gene
Findings suggest that dietary advice may need to be tailored to individual's digestive system.
An Alternative Suite of Universal Primers for Genotyping in Multiplex PCR
The new set of universal primers for multiplex genotyping were proven to be efficient and reliable in varied species.
DNA Polymerase Preference Determines PCR Priming Efficiency
Researchers use high throughput sequencing to systematically demonstrate and characterize DNA polymerase priming bias.
Droplet Digital PCR Enables Measurement of Potential Cancer Survival Biomarker
Study paves the way for further research into the role of TIL quantification in immunotherapy and as a cancer survival predictor.

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