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Tuesday, July 22, 2014
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Determination of Interferon-Pathway–Related Gene Induction during RNAi
Jie Kang, Peter Hahn, Frank Narz, Cornelia Schmidt, Kirsten Haussuehl, Dong Liang, Subu Yerramilli, Eric Lader and Dirk Löffert,

Experimental approaches using short interfering RNA (siRNA) molecules to specifically silence gene expression have become more widely used in recent years. As for all techniques in molecular and cellular biology, the importance of protocol optimization and the use of appropriate controls cannot be overestimated.

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The BioRobot® Universal System - Multiple Applications on one Instrument
Thomas Weierstall, Anja Schultz, Daniel Langendörfer, Martin Heller and Carola Schade

The BioRobot Universal System enables standardized sample preparation and reaction setup using optimized, ready-to-run protocols. Fully tested protocols provide purification of RNA from blood, cells, and tissue and purification of genomic DNA from blood and buccal swabs, as well as RT-PCR and PCR setup.

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Incorporation of Nanolitre Pipetting Technology into Medium and High Throughput SNP Genotyping Platforms at KBiosciences
Phil Robinson, Aoife Gallagher

This poster will describe the current process employed by KBiosciences. The vital role spot-on™ technology, incorporated into the Equator™ benchtop robotic liquid handling instrument, has played in improving performance and reducing costs are highlighted.

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Quantitative Measurement of microRNAs Reveals Distinct Correlation with Acute Myeloid Leukemia (AML) Signature
Silvana Debernardi, Spyros Skoulakis, Gael Molloy, Bryan D. Young

Using a novel quantitative real-time PCR assay, specific to the mature product, the expression level of a selected group of miRNAs was measured across a set of 30 primary adult acute myeloid leukemia (AML) with a normal karyotype.

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Showing Results 81 - 84 of 84
Scientific News
Determination of Arabidopsis Thaliana Telomere Length by PCR
Researchers have designed two telomeric degenerated primers that amplify Arabidopsis telomeres by MMQPCR.
Methods for Multiplex Template Sampling in Digital PCR Assays
This article presents Multiplex Template Sampling, an alternative strategy to effectively increase template concentrations in dPCR analysis.
Detection of Large Expansions in Myotonic Dystrophy Type 1
The aims of this study were to verify the validity of triplet primed-PCR for the diagnosis of patients presenting with DM1 clinical findings and to simplify the testing procedure.
The 2014 Annual MO BIO Microbiome Awards, Offering More Than $10,000 of Prizes
The Microbiome Awards aim to provide young, extraordinary scientists with funding and recognition to carry out scientific work in the field of microbiome research.
Genetic Discovery Could Aid Diagnosis of Childhood TB
A distinctive genetic 'signature' found in the blood of children with TB offers new hope for improved diagnosis of the disease.
Genotoxicity Evaluation of Acephate and Profenofos by the PCR-RFLP Assay
The rampant use of organophosphorus insecticides has created a chemical environment which is proving harmful to the living system.
Strong Link Between Obesity and 'Carb Breakdown' Gene
Findings suggest that dietary advice may need to be tailored to individual's digestive system.
An Alternative Suite of Universal Primers for Genotyping in Multiplex PCR
The new set of universal primers for multiplex genotyping were proven to be efficient and reliable in varied species.
DNA Polymerase Preference Determines PCR Priming Efficiency
Researchers use high throughput sequencing to systematically demonstrate and characterize DNA polymerase priming bias.
Droplet Digital PCR Enables Measurement of Potential Cancer Survival Biomarker
Study paves the way for further research into the role of TIL quantification in immunotherapy and as a cancer survival predictor.

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