|MicroRNA Profiling of Breast Cancer using miRCURY™ LNA Arrays|
Thomas Litman, Mikkel Nørholm, Christian Glue, Nina Stahlberg, Nana Jacobsen, Jens Eriksen, Inge M Svane, Henrik Flyger, Eva Balslev, Carsten Alsb and Søren Møller
MicroRNAs (miRNAs) comprise a recently identified class of small, non-protein-coding regulatory molecules that play important roles in many physiologic and pathologic processes, including differentiation, viral infection, and oncogenesis. In cancer, abnormal miRNA expression suggests that these molecules may serve as valuable diagnostic and prognostic molecular signatures.
|Determination of Interferon-Pathway–Related Gene Induction during RNAi |
Jie Kang, Peter Hahn, Frank Narz, Cornelia Schmidt, Kirsten Haussuehl, Dong Liang, Subu Yerramilli, Eric Lader and Dirk Löffert,
Experimental approaches using short interfering RNA (siRNA) molecules to specifically silence gene expression have become more widely used in recent years. As for all techniques in molecular and cellular biology, the importance of protocol optimization and the use of appropriate controls cannot be overestimated.
|The BioRobot® Universal System - Multiple Applications on one Instrument|
Thomas Weierstall, Anja Schultz, Daniel Langendörfer, Martin Heller and Carola Schade
The BioRobot Universal System enables standardized sample preparation and reaction setup using optimized, ready-to-run protocols. Fully tested protocols provide purification of RNA from blood, cells, and tissue and purification of genomic DNA from blood and buccal swabs, as well as RT-PCR and PCR setup.
|Incorporation of Nanolitre Pipetting Technology into Medium and High Throughput SNP Genotyping Platforms at KBiosciences|
Phil Robinson, Aoife Gallagher
This poster will describe the current process employed by KBiosciences. The vital role spot-on™ technology, incorporated into the Equator™ benchtop robotic liquid handling instrument, has played in improving performance and reducing costs are highlighted.
|Quantitative Measurement of microRNAs Reveals Distinct Correlation with Acute Myeloid Leukemia (AML) Signature|
Silvana Debernardi, Spyros Skoulakis, Gael Molloy, Bryan D. Young
Using a novel quantitative real-time PCR assay, specific to the mature product, the expression level of a selected group of miRNAs was measured across a set of 30 primary adult acute myeloid leukemia (AML) with a normal karyotype.
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