|Real-Time Multiplex Rt-PCR on Circulating Tumor Cells|
Anieta M. Sieuwerts, Stefan Sleijfer, Jaco Kraan, Joan Bolt-de Vries, Jan-Willem Gratama, John WM Martens and John A. Foekens
Using the CTC kit (CellSearch™), cells that attached to anti-EpCAM Moab were immunomagnetically separated and used for analysis of a selected pilot set of 32 genes by real time RT-PCR. This study shows the feasibility of multiple gene expression analysis on RNA isolated from only one tumor cell. Most importantly, expression analysis of several tumor-specific genes in blood samples containing only 2 tumor cells is already possible.
|Novel Fluidics Microbead Trap/Flow Cell Enhances Speed/Sensitivity of Bead-Based Bioassays Up to 5-Fold|
RM Ozanich, CJ Bruckner-Lea, JW Grate, MG Warner, BP Dockendorff, KC Antolick, HC Edberg, LH Johnson, AN Easterday
Pacific Northwest National Laboratory (PNNL) has developed a micro/nano particle trap that allows surface-functionalized magnetic or non-magnetic particles to be trapped with subsequent perfusion of sample, reagents and wash solutions, yielding significant (up to 5-fold) improvements in assay speed and sensitivity, while significantly reducing sample matrix effects.
|Utilizing High Speed Photography to Optimize Low Volume Dispensing Conditions |
Mary Cornett, Mitch Gordon and Anca Rothe
In this study we use high-speed photography as a feedback mechanism for adjusting the Nanodrop instrument dispense settings to improve the positional dispense accuracy of low volume (nanoliter) drops. These same parameters can be investigated, with various fluid classes, to reduce deleterious effects on dispensing performance such as deflected streams, satellite formation, secondary pulses and drop deformation.
|MicroRNA Profiling of Breast Cancer using miRCURY™ LNA Arrays|
Thomas Litman, Mikkel Nørholm, Christian Glue, Nina Stahlberg, Nana Jacobsen, Jens Eriksen, Inge M Svane, Henrik Flyger, Eva Balslev, Carsten Alsb and Søren Møller
MicroRNAs (miRNAs) comprise a recently identified class of small, non-protein-coding regulatory molecules that play important roles in many physiologic and pathologic processes, including differentiation, viral infection, and oncogenesis. In cancer, abnormal miRNA expression suggests that these molecules may serve as valuable diagnostic and prognostic molecular signatures.
|Determination of Interferon-Pathway–Related Gene Induction during RNAi |
Jie Kang, Peter Hahn, Frank Narz, Cornelia Schmidt, Kirsten Haussuehl, Dong Liang, Subu Yerramilli, Eric Lader and Dirk Löffert,
Experimental approaches using short interfering RNA (siRNA) molecules to specifically silence gene expression have become more widely used in recent years. As for all techniques in molecular and cellular biology, the importance of protocol optimization and the use of appropriate controls cannot be overestimated.
|The BioRobot® Universal System - Multiple Applications on one Instrument|
Thomas Weierstall, Anja Schultz, Daniel Langendörfer, Martin Heller and Carola Schade
The BioRobot Universal System enables standardized sample preparation and reaction setup using optimized, ready-to-run protocols. Fully tested protocols provide purification of RNA from blood, cells, and tissue and purification of genomic DNA from blood and buccal swabs, as well as RT-PCR and PCR setup.
|Incorporation of Nanolitre Pipetting Technology into Medium and High Throughput SNP Genotyping Platforms at KBiosciences|
Phil Robinson, Aoife Gallagher
This poster will describe the current process employed by KBiosciences. The vital role spot-on™ technology, incorporated into the Equator™ benchtop robotic liquid handling instrument, has played in improving performance and reducing costs are highlighted.
|Quantitative Measurement of microRNAs Reveals Distinct Correlation with Acute Myeloid Leukemia (AML) Signature|
Silvana Debernardi, Spyros Skoulakis, Gael Molloy, Bryan D. Young
Using a novel quantitative real-time PCR assay, specific to the mature product, the expression level of a selected group of miRNAs was measured across a set of 30 primary adult acute myeloid leukemia (AML) with a normal karyotype.
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